Abstract：
:A novel glycosaminoglycan-like sulfated polysaccharide (AAP) from the pleopods of Haliotis discus hannai Ino was purified by DEAE ion exchange chromatography followed with S-300 HR geltrion chromatography. Chemical composition analysis indicated that AAP was composed of galactosamine, glucuronic acid, fucose, galactose with a ratio of 2.14:2.37:2.94:1, the content of sulfate was 15.5%. The average molecular weight (M(w)) was 56.2 kDa in a TSK G4000 column. Further IR, 1D, 2D NMR spectroscopic analysis of the correlation signals of different sugar units gave a proposal repeating structure, as follows: [chemical structure: see the text] In vitro anticoagulant assay indicated AAP prolonged both the activated partial thromboplastin time (APTT) and thrombin time (TT), with a 22.5 U/mg and 72.0 U/mg compared with standard heparin, respectively. The anticoagulant property of AAP was mainly attributed to powerful potentiation thrombin by anti-thrombin III.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Li G,Chen S,Wang Y,Xue Y,Chang Y,Li Z,Wang J,Xue Cdoi
10.1016/j.ijbiomac.2011.09.017subject
Has Abstractpub_date
2011-12-01 00:00:00pages
1160-6issue
5eissn
0141-8130issn
1879-0003pii
S0141-8130(11)00365-5journal_volume
49pub_type
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