Interaction of two fragments of cibacron blue F3GA having anthraquinone chromophores with basic polypeptides.

Abstract:

:The interaction of two anthraquinone dyes, bromaminic acid 1-amino-4-bromo anthraquinone-2-sulphonic acid (BA) and 1-amino-4-(4'-aminophenylamino)-anthraquinone-2,3'-disulphonic acid (ASSO), with poly-L-lysine (PLL), poly-L-ornithine (PLO) and poly-L-arginine (PLA) has been studied. The flexibility of the side chain (chain length) and the geometry of the charge centre (tetrahedral/planar) of the polypeptide were found to affect the interaction. Further, both dyes induce ordered conformation (alpha or beta) for the three polypeptides, but the final conformation was found to depend on the polypeptide as well as the number of anionic sites on the ligand. While the bidentate ASSO-polypeptide complexes acquire beta-conformation, the monodentate BA distinguished between the three substrates and induces beta-conformation only for PLO.

journal_name

Int J Biol Macromol

authors

Ratna MA,Atreyi M,Rao MV

doi

10.1016/s0141-8130(05)80028-5

keywords:

subject

Has Abstract

pub_date

1992-08-01 00:00:00

pages

201-9

issue

4

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(05)80028-5

journal_volume

14

pub_type

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