Abstract：
:A gene, encoding a new alginate lyase Algb, was identified and cloned from marine bacterium Vibrio sp. W13. The recombinant alginate lyase was characterized followed by being purified on Ni-NTA Sepharose. It exhibited the highest activity (457 U/mg) at pH 8.0 and 30 °C. Interestingly, Algb possessed broader substrate specificity. It showed activities toward both polyM (poly β-D-mannuronate) and polyG (poly α-L-guluronate). Furthermore, K(m) values of Algb toward alginate (0.67 mg/ml) and polyMG (0.50 mg/ml) are lower than those toward polyG (1.04 mg/ml) and polyM (6.90 mg/ml). The TLC and ESI-MS analysis suggested that Algb mainly released oligosaccharides with DP of 2-5 from the four kinds of substrates in an endolytic manner. Therefore, it may be a potent tool to produce alginate oligosaccharides with low DP.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhu B,Tan H,Qin Y,Xu Q,Du Y,Yin Hdoi
10.1016/j.ijbiomac.2015.01.053subject
Has Abstractpub_date
2015-04-01 00:00:00pages
330-7eissn
0141-8130issn
1879-0003pii
S0141-8130(15)00064-1journal_volume
75pub_type
杂志文章abstract：:Cotton is among the most important fiber crops for the textile-based industry, thanks to its cellulose-rich mature fibers. The fiber initiation and elongation are one of the best models for deciphering mechanisms of single-cell differentiation and growth, that also target of fiber development programs. During the last...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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abstract：:Extracellular alkaline protease producing salt tolerant alkaliphilic actinobacteria, Nocardiopsis xinjiangensis strain OM-6 was isolated from Okha Madi (OM) site of coastal Gujarat, India. The purified protease was stable even at 70°C in 100mM Ca2+ with Kd=20×10-3 and t1/2=34min. The activation energies (E), enthalpy ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:FT-IR and X-ray analyses were employed to determine the relative ratio of cellulose Ialpha and Ibeta crystalline phases present in each developmental stage of coniferous tracheid cell wall formation. The IR spectra showed that initially the Ialpha phase occupies 50% of the crystalline regions in the primary cell wall ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(98)00065-8
更新日期：1999-01-01 00:00:00
abstract：:Effect of Zn(2+) ions on DNA transition from B-form to a metallized form (m-DNA) in Tris and tetraborate buffers at pH 8.5 has been studied by visible and differential UV-spectroscopy and by thermal denaturation. The results have been compared to those obtained at pH 6.5 in cacodylate buffer. It was found that in alka...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2011.11.011
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abstract：:The aim of this study was to develop a new bioactive gelatin-oxidized starch nanofibers containing Lawsonia Inermis (henna) for treating second-degree burn wounds. Continuous, smooth, and bead-free fibers were obtained when the gelatin-starch ratio was 70/30.Additionally, the fiber dimeter was reduced with increasing ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.061
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abstract：:Protein misfolding, followed by aggregation and amyloid formation is an underlying pathological hallmark in a number of prevalent diseases, including Parkinson's (PD), Alzheimer's (AD) and Type 2 diabetes (T2D). In the case of PD, the aggregation of α-synuclein protein (α-syn) has been shown to be highly cytotoxic and...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2016-02-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.148
更新日期：2018-12-01 00:00:00
abstract：:In this study, two sulfated polysaccharides (S-CP1-4 and S-CP1-8) from Cyclocarya paliurus were produced by chlorosulfonic acid-pyridine method. Hydrogen peroxide (H2O2) was used to develop an oxidative stress model in the mouse macrophage cell line RAW264.7. Effects of the two sulfated polysaccharides on H2O2-induced...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.06.031
更新日期：2015-09-01 00:00:00
abstract：:The present work reports the successful preparation, thermal and mechanical characterization of high performance films of Na(+) montmorillonite (MMT)/graphene oxide (GO)/chitosan (CS) composite using simple solution mixing evaporation method. The formations of films were verified by Fourier transform infrared (FT-IR) ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.05.055
更新日期：2015-08-01 00:00:00
abstract：:In this study, a novel and efficient aptasensor based on immobilization of thiol terminated prostate specific antigen (PSA) binding DNA aptamer onto Au nanoparticles/fullerene C60-chitosan-ionic liquid/multiwalled carbon nanotubes/screen printed carbon electrode has been fabricated for ultrasensitive aptasensing of bi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.01.012
更新日期：2019-04-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.04.047
更新日期：2020-08-01 00:00:00
abstract：:Considering alarming projections in the prevalence of periodontitis, following study was undertaken to develop chitosan-vanillin crosslinked microspheres loaded in-situ gel (MLIG) implants containing ornidazole and doxycycline hyclate for the treatment of pocket infections. Firstly, microspheres were formulated and op...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
abstract：:A water-soluble polysaccharide was isolated from marine green algae Ulva pertusa and then chelated with iron to prepare the polysaccharide‑iron (III) complex. The immunomodulatory activities of sulfated polysaccharide and polysaccharide‑iron (III) complex were investigated through a mice immune-deficiency model. Cyclo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.101
更新日期：2019-07-15 00:00:00
abstract：:Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a ubiquitous enzyme involved in glycolysis. It is also referred to as a moonlighting protein as it has many diverse functions like regulation of apoptosis, iron homeostasis, cell-matrix interactions, adherence to human colon etc. apart from its principal role in glyc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.04.041
更新日期：2016-10-01 00:00:00
abstract：:Modified starches play a crucial role in the pharmaceutical industries in controlling the drug release at a pre-determined rate. The effect of acetylation on the physicochemical and drug release characteristics of the starches from five different Indian L. siceraria cultivars was investigated. Starches isolated from t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.10.007
更新日期：2015-01-01 00:00:00
abstract：:Fibrinogen (Fg) also known as coagulation factor I represents about 4% of the total human plasma proteins. The main function of Fg is its involvement in last phase of blood coagulation cascade, when thrombin-induced conversion of dissolved plasma fibrinogen into an insoluble fibrin clot occurs. The reaction of fibrino...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.01.019
更新日期：2013-04-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.04.001
更新日期：2009-07-01 00:00:00
abstract：:In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method. The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of P...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.07.074
更新日期：2018-10-15 00:00:00
abstract：:Bio-nanocomposites, composed of biopolymers and inorganic solids, show dimensions in the nanometer range (1-100nm) which can be widely used in variety of areas owing to multidimensional properties like biocompatibility, antimicrobial activity and biodegradability. Considering these versatile properties of the bio-nano...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2016.07.102
更新日期：2016-11-01 00:00:00
abstract：:The objective of current study was to examine the electrostatic interactions between gelatin and carboxymethyl cellulose (CMC) as a function of pH and mixing ratio (MR) and to observe how the physical and mechanical properties of gelatin-CMC composite films are affected by these interactions. The interaction between b...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.079
更新日期：2018-07-15 00:00:00
abstract：:3D porous composites based on blends of chitosan, collagen and hyaluronic acid were obtained through the lyophilization process. Mechanical properties, swelling behavior and thermal stability of the blends were studied. Moreover, SEM images were taken and the structure of the blends was studied. Biological properties ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.04.085
更新日期：2016-08-01 00:00:00
abstract：:There have been few reports of complete mitochondrial genomes (mitogenomes) of scale insects, and it has been indicated that complex and novel structures in their mitogenomes may lead to difficulties in sequencing, assembly and annotation. Transfer RNAs (tRNAs) usually possess typical cloverleaf secondary structures, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.04.257
更新日期：2020-05-07 00:00:00
abstract：:Despite the use of layered double hydroxides (LDH) in different electrochemical (bio)sensors, the construction of aptasensors using LDH-based surfaces was not reported to the best of our knowledge. This may be due to the lack of a suitable linker to attach aptamers to the LDH-modified surface. LDH-based aptasensors ar...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
abstract：:Use of biological macromolecules for wastewater remediation process has become the topic of intense research mostly driven by growing concerns about the depletion of petroleum oil reserves and environmental problems. So in view of technological significance of cellulosic biopolymers in various fields, the present stud...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.03.046
更新日期：2014-06-01 00:00:00
abstract：:Iron oxide/cellulose high-performance nanocomposite film is successfully prepared by impregnation of iron oxide nanoparticles into a regenerated cellulose film. The structure, thermal stability and mechanical properties of the nanocomposite films are investigated by the wide-angle X-ray diffraction, Fourier transform ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.11.042
更新日期：2015-03-01 00:00:00
abstract：:The single Cys residue in the C-terminal domain of bovine eye lens alpha-crystallin was covalently labelled with 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone. This novel SH-reactive two-band ratiometric fluorescent dye is characterized by excited state intramolecular proton transfer reaction yielding two highly emiss...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.07.007
更新日期：2005-09-28 00:00:00
abstract：:Klebsiella pneumoniae strain KK207-2 was isolated in 2010 from a bloodstream infection of an inpatient at an Italian hospital. It was previously found to produce the KPC-2 carbapenemase and to belong to clade 1 of sequence type 258. Genotyping of the conserved wzi and wzc genes from strain KK207-2 yielded contrasting ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.02.128
更新日期：2019-06-01 00:00:00
abstract：:Temporomandibular disorder is a clinical painful condition in the temporomandibular joint (TMJ) region. The purified sulfated polysaccharide from the green marine algae Caulerpa racemosa (Cr) has provided anti-inflammatory and antinociceptive activity. This study evaluated these effects on a TMJ hypernociception model...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.01.272
更新日期：2020-05-01 00:00:00
abstract：:In this work, 1,3,5-triazine-2,4,6-tribenzaldehyde was synthesized and chosen as the cross-linking agent for preparation of novel thermo- and pH-responsive hydrogels based on chitosan. The cross-linking proceeds through formation of imine bond by reaction of amino groups of chitosan with aldehyde groups of the cross-l...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.07.128
更新日期：2017-12-01 00:00:00
abstract：:Ultraviolet radiation is a risk factor for cataractogenesis. It is believed that enhanced rates of lens opacification and cataract formation are the results of gradual loss of chaperone-like efficiency of α-crystallin upon exposure to UV light. To characterize chaperone-like activity of α-crystallin damaged by UV irra...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.10.060
更新日期：2015-02-01 00:00:00
