Abstract:
:In this study, a novel and efficient aptasensor based on immobilization of thiol terminated prostate specific antigen (PSA) binding DNA aptamer onto Au nanoparticles/fullerene C60-chitosan-ionic liquid/multiwalled carbon nanotubes/screen printed carbon electrode has been fabricated for ultrasensitive aptasensing of biomarker PSA. Formation of PSA-aptamer complex caused a variation in electrochemical impedance spectroscopic (EIS) and differential pulse voltammetric (DPV) responses of the aptasensor which enabled us to aptasensing of the PSA by EIS and DPV methods. Morphology and electrochemical properties of the fabricated aptasensor were examined by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS. The aptasensor was successfully applied to the determination of PSA by EIS and DPV in the range of 1-200 pg mL-1 with a limit of detection (LOD) of 0.5 pg mL-1 and 2.5-90 ng mL-1 with a LOD of 1.5 ng mL-1, respectively. This aptasensor exhibited outstanding anti-interference ability towards co-existing molecules with good stability, sensitivity, repeatability and reproducibility. Practical application of the aptasensor was examined with analysis of the PSA levels in serum samples obtained from patients with prostate cancer using both the aptasensor and a reference method. The results revealed the proposed system to be a promising candidate for clinical analysis of PSA.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Jalalvand ARdoi
10.1016/j.ijbiomac.2019.01.012subject
Has Abstractpub_date
2019-04-01 00:00:00pages
1065-1073eissn
0141-8130issn
1879-0003pii
S0141-8130(18)36802-8journal_volume
126pub_type
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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