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Efficient immobilization of aptamers on the layered double hydroxide nanohybrids for the electrochemical proteins detection.

Abstract：

:Despite the use of layered double hydroxides (LDH) in different electrochemical (bio)sensors, the construction of aptasensors using LDH-based surfaces was not reported to the best of our knowledge. This may be due to the lack of a suitable linker to attach aptamers to the LDH-modified surface. LDH-based aptasensors are established here as very sensitive and reliable devices in serum and cerebrospinal fluid (CSF) analysis. 5'-NH2 DNA aptamer probes were immobilized on the LDH-based surfaces in a vertical conformation without any linker materials. Due to the low electron conductivity of the LDH, carbon nanotubes (CNT) with high electronic conductivity and high surface area were combined with LDH. Thrombin was used as a model protein for aptasensing. The sensor shows a linear range of 0.005-12,000 pmol L-1 and a limit of detection of 0.1 fmol L-1. Moreover, the aptasensor was used for the sensing of thrombin in CSF and serum samples acquired from both healthy and patients with different disease.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Konari M,Heydari-Bafrooei E,Dinari M

doi

10.1016/j.ijbiomac.2020.10.063

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

54-60

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)34694-8

journal_volume

166

pub_type

杂志文章

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