Abstract：
:Recently, protein purification methods have a very wide area of research. Many of these methods are both expensive and multi-stage methods, that are needed in specific equipment. In this study, biopolymer coated magnetic nanoparticles, carboxymethyl cellulose (CMC) and chitosan (CH) coated Fe3O4 (magnetite) nanocomposites, are used in a new purification process. The structure of the synthesized magnetic nanocomposites were characterized by Fourier transform infrared (FTIR) spectrometry, X-ray diffraction (XRD), scanning electron microscope (SEM), transmission electron microscope (TEM), thermogravimetric (TGA) analysis and zeta potential for surface charge of magnetic nanocomposites. Molecular weight and purification degree of peroxidase were estimated with SDS-PAGE. Peroxidase enzyme was purified a yield of 82.55% with carboxymethyl cellulose and 76.72% with chitosan using this method.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zengin Kurt B,Uckaya F,Durmus Zdoi
10.1016/j.ijbiomac.2016.12.042subject
Has Abstractpub_date
2017-03-01 00:00:00pages
149-160eissn
0141-8130issn
1879-0003pii
S0141-8130(16)31907-9journal_volume
96pub_type
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