Abstract：
:Thiothrix strains are filamentous sulfur-oxidizing bacteria common in activated sludge. Some of the members, including Thiothrix nivea and T. fructosivorans, are known to form a microtubular sheath that covers a line of cells. The sheaths are assemblages of [→4)-β-d-GlcN-(1→4)-β-d-Glc-(1→]n modified with unusual deoxy sugars. In an attempt to elucidate the sheath-forming mechanism, the patterns of sheath formation and cell proliferation were determined in this study. Prior to analysis, both sheaths were confirmed to be highly de-N-acetylated. Sheaths in viable filaments were N-biotinylated followed by cultivation and then fluorescently immunostained. Epifluorescence microscopy of the filaments revealed ubiquitous elongation of the sheaths. For visualization of the cell proliferation pattern, the cell membrane was fluorescently stained. The epifluorescence images demonstrated that cell proliferation also proceeds ubiquitously, suggesting that sheath elongation proceeds surrounding an elongating cell. In addition, the fine structure of the Thiothrix filaments was analyzed by transmission electron microscopy employing a freeze-substitution technique. The micrographs of freeze-substituted filaments showed that the sheaths were thin and single layered. In contrast, the sheaths in chemically fixed filaments appeared thick and multilayered. Treatment with glutaraldehyde probably caused deformation of the sheaths. Supporting this possibility, the sheaths were found to be deformed or solubilized by N-acetylation.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kawasaki Y,Endo T,Fujiwara A,Kondo K,Katahira M,Nittami T,Sato M,Takeda Mdoi
10.1016/j.ijbiomac.2016.11.025subject
Has Abstractpub_date
2017-02-01 00:00:00pages
1280-1288eissn
0141-8130issn
1879-0003pii
S0141-8130(16)31371-Xjournal_volume
95pub_type
杂志文章abstract：:This study aims to prepare binary edible films made from carboxymethyl cellulose (CMC) and chitosan biguanidine hydrochloride (CBg) activated with frankincense oil (FO). The interactions between CMC, CBg and FO were confirmed by FTIR. XRD showed that the addition of FO led to decreasing the crystallinity of CMC/CBg fi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.104
更新日期：2019-10-15 00:00:00
abstract：:Limited proteolysis of streptokinase (Sk) by trypsin and thermolysin was performed under various incubation conditions and analysed by polyacrylamide gel electrophoresis. Several fragments (Sk1, Tr27, Tr17, Th26, and Th16) were isolated and characterized further. The N-terminal sequences of Tr27, Tr17, Th26, Th16 and ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(92)90007-u
更新日期：1992-04-01 00:00:00
abstract：:Non-enzymatic degradation of wood via a chelator-mediated Fenton (CMF) system is the primary method for initial attack in brown rot fungal decomposition of wood, the most common type of fungal degradation of terrestrial carbon biomass on the planet. In this study, the degradation of thin films of cellulose and chitin ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.262
更新日期：2020-06-15 00:00:00
abstract：:Five water-soluble chitosan derivatives were carried out by quaternizing either iodomethane or N-(3-chloro-2-hydroxypropyl) trimethylammonium chloride (Quat188) as a quaternizing agent under basic condition. The degree of quaternization (DQ) ranged between 28±2% and 90±2%. The antifungal activity was evaluated by usin...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2011.11.004
更新日期：2012-01-01 00:00:00
abstract：:In this study, the sulfated polysaccharide (SPs) was isolated from Spirulina platensis. The isolated SPs contains carbohydrate, sulfate, protein and uronic acid at 38.7 ± 0.30%, 21.3 ± 0.87%, 7.1 ± 0.15% and 7.9 ± 0.4% respectively. The elemental analysis confirmed the presence of carbon (18.01 ± 0.10%), hydrogen (1.8...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.09.024
更新日期：2019-12-01 00:00:00
abstract：:An analysis of the possible modes of motion of water molecules in polysaccharide gels indicates that the interpretation of NMR results does not require the supposition of a class of 'bound water' with highly perturbed motional states. There may, however, be water molecules associated with junction zones in the gels wh...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(97)00045-7
更新日期：1997-08-01 00:00:00
abstract：:The effects of the incorporation of PLA-β-cyclodextrin-inclusion complex (IC) and β-cyclodextrin (β-CD) on biopolyester PLA films were investigated. Thermal stability, surface morphology, barrier, and mechanical properties of the films were measured at varying IC (1, 3, 5, and 7%) and β-CD (1 and 5%) concentrations. T...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.08.036
更新日期：2015-11-01 00:00:00
abstract：:Dilute solution properties of an unknown starch are important to understand its performance and applications in food and non-food industries. In this paper, rheological and molecular properties (intrinsic viscosity, molecular weight, shape factor, voluminosity, conformation and coil overlap parameters) of the starches...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.02.050
更新日期：2016-06-01 00:00:00
abstract：:Chitosan (300 kDa) was degraded by cellulase to chitosans with molecular weights (MWs) of 156, 72, 7.1, and 3.3 kDa and a chitooligosaccharide mixture (COS). Effects of these on NO secretion, cytokine production, and mitogen-activated protein kinase pathways in lipopolysaccharide (LPS)-induced murine RAW 264.7 macroph...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.02.066
更新日期：2019-06-15 00:00:00
abstract：:The ever-increasing applications of enzymes are limited by the relatively poor performance in harsh processing conditions. As a result, there are constant innovations in immobilization protocols for improving biocatalyst activity and stability. Bacterial spores are cheap to generate and highly resistant to environment...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.10.171
更新日期：2021-01-01 00:00:00
abstract：:Bone damage, due to congenital defects, trauma and sports-related injuries, has become a foremost health problem all over the world. The present study involves fabrication of a nanocomposite scaffold of graphene oxide (GO), gelatin and alginate, with an aim of enhancing bone regeneration. The effect of varying concent...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.113
更新日期：2019-07-15 00:00:00
abstract：:In this study, the inhibitory effects of four anthraquinones including chrysophanol, emodin, physcione and rhein on tyrosinase were investigated by enzyme inhibition assay. The results indicated that all of anthraquinones could significantly inhibit the activity of tyrosinase in a competitive manner. To gain insight i...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.193
更新日期：2020-10-01 00:00:00
abstract：:Leaching and re-absorption of leached solids and changes in kernel structure are of prime importance for the quality of cooked rice. This paper describes the contents and structural changes of both starch and protein in leached solids and rice kernels. Under actual cooking conditions, the leached solids increased with...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.080
更新日期：2020-03-15 00:00:00
abstract：:Transdermal drug delivery systems are topically administered medicaments in the form of patches that deliver drugs for systemic effects at a predetermined and controlled rate. It works very simply in which drug is applied inside the patch and it is worn on skin for long period of time. Polymer matrix, drug, permeation...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.05.062
更新日期：2014-08-01 00:00:00
abstract：:Three 4-(1H-imidazo[4,5-f]-1,10-phenanthrolin-2-yl)phenol derivatives-based platinum (II) complexes have been synthesized, and their G-quadruplex DNAs-binding interactions, telomerase inhibition, antiproliferative activity, and cell cycle arrest were studied. Three complexes show the preference for stabilizing h-telo,...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.01.004
更新日期：2013-04-01 00:00:00
abstract：:The current research work focuses on the medical application of the cost-effective cross-linked starch nanoparticles, for the transdermal delivery using Diclofenac sodium (DS) as a model drug. The prepared DS-cross-linked starch nanoparticles were synthesized using nanoprecipitation technique at different concentratio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.09.005
更新日期：2015-11-01 00:00:00
abstract：:Hydrogels based on pectin and cellulose nanocrystals (CNC) were used in our study to nucleation and growth of hydroxyapatite (HAp) by the biomimetic method. In this study, we evaluated the direct impact of the different percentages of CNC on pectin hydrogel and the influence of HAp obtained through two methods. CNC we...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.012
更新日期：2021-01-15 00:00:00
abstract：:Calorimetry was used to measure the rates of irreversible denaturation of collagen in intact mammalian lens capsules, isothermally stored in water at different temperatures, and the kinetic characteristics of the process determined. At 47.4 degrees C, the number of native collagen molecules declined by a factor of 10 ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(93)90025-h
更新日期：1993-10-01 00:00:00
abstract：:Collagen is considered to be one of the most useful biomaterials with different medical applications. However, collagen properties differ from one source to another. The aim of this study was to extract, purify, characterize and perform preliminary biological evaluation of type I collagen from scales of Egyptian Nile ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.05.019
更新日期：2015-08-01 00:00:00
abstract：:The formation of poly(beta-hydroxybutyrate), PHB, by Rhodobacter sphaeroides and Alcaligenes eutrophus was studied using the following carbon sources and solvents: (1), acetate in H2O; (2), D3-acetate in H2O; (3), acetate in 90 to 92% D2O; and (4), D3-acetate in 90 to 92% D2O. The growth of Rb. sphaeroides cultured un...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(05)80017-0
更新日期：1992-02-01 00:00:00
abstract：:DNA vaccine is an important tool to elicit both humoral and cellular immunity. Present study investigates mucosal immune response of Labeo rohita (15 ± 04 g) to plasmid DNA (pDNA) vaccine macromolecule complexed with nanoparticles (NPs). Poly lactic-co-glycolic acid (PLGA), Chitosan (Chit) and PLGA-Chit-NPs were synth...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.04.048
更新日期：2020-08-01 00:00:00
abstract：:Electrospinning technique was used to fabricate chloroamphenicol loaded poly (L-lactic acid)/ZrO2 (CP-PLLA/ZrO2) nanofibrous membranes. The average diameter of drug carrier PLLA/ZrO2 nanofibers increased with ZrO2 concentration increasing and ribbon-shape fibers were present when ZrO2 content reached 3% and above. The...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.10.002
更新日期：2013-11-01 00:00:00
abstract：:The aim of this study is to probe new functions of a polysaccharide from Spirulina platensis (PSP) on constipation and intestinal microbiota in mice. Diphenoxylate-induced constipation in mice was treated with different doses of PSP, followed by examining the defecation patterns, levels of acetyl cholinesterase (AchE)...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.209
更新日期：2019-07-15 00:00:00
abstract：:A C6-carboxylated curdlan (C6-Cc) obtained from 4-acetamido-TEMPO-mediated oxidation of curdlan was used both as a reducing and stabilizing agent for green synthesis of pH-responsive AuNPs, which was carried out by controlling the pH of the C6-Cc solution at a high temperature (100°C). C6-Cc presented a semi-flexible ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.08.029
更新日期：2018-01-01 00:00:00
abstract：:The eggs of black widow spider (L. tredecimguttatus) have been demonstrated to be rich in biologically active components that exhibit great research value and application foreground. In the present study, a protein toxin, named Latroeggtoxin-II, was isolated from the eggs using the combination of gel filtration, ion e...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.01.004
更新日期：2014-04-01 00:00:00
abstract：:Bacterially synthesized poly(hydroxyalkanoate)s (PHAs) suffers from low crystallization rate which is enhanced by using tailor-made oxalamide compounds as nucleators. The influence of nucleator configurations on the crystallization behaviour of the PHAs was investigated using differential scanning calorimetry (DSC), p...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.06.001
更新日期：2017-11-01 00:00:00
abstract：:Accumulating evidence has revealed the imprinting of insulin-like growth factor-2 gene (IGF2) is maintained by binding of CCCTC binding factor (CTCF) to the unmethylated imprinting control region (ICR) between IGF2 and H19 genes. We have previously reported that high-density lipoprotein binding protein (HDLBP/vigilin)...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.11.109
更新日期：2018-03-01 00:00:00
abstract：:The present study investigates the purification, structure and in vitro antioxidant activities of a novel water soluble polysaccharide (LWSP) extracted from Linum usitatissimum L. seeds and evaluates the in vivo wound healing performance on CO2 laser fractional burn in a rat model. LWSP is a heteropolysaccharide that ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.077
更新日期：2020-02-01 00:00:00
abstract：:In this study, highly porous N/S doped magnetic carbon aerogel (N/S-MCA) was successfully fabricated using sugarcane bagasse based cellulose and used for the removal of bisphenol‑A (BPA) from aqueous solution. The fabricated N/S-MCA nanocomposite derived from cellulose was characterized by various analytical technique...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.004
更新日期：2019-07-01 00:00:00
abstract：:Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects. In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels. The hydrogels were lyophilized and t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.179
更新日期：2018-12-01 00:00:00
