In vitro DNA binding studies of therapeutic and prophylactic drug citral.

Abstract:

:The study of drug-DNA interactions is of great importance, as it paves the way towards the design of better therapeutic agents. Here, the interaction of DNA with a therapeutic and prophylactic drug citral has been studied. We have attempted to ascertain the mode of binding of citral with calf thymus DNA (Ct-DNA) through various biophysical techniques. Analysis of the UV-visible absorbance spectra and fluorescence spectra indicated the formation of a complex between citral and Ct-DNA. Competitive binding assays with ethidium bromide (EB), acridine orange (AO) and Hoechst 33258 reflected that citral possibly intercalates within the Ct-DNA. These observations were further confirmed by circular dichroism (CD) spectral analysis, viscosity measurements, DNA melting and molecular docking studies. This study is expected to contribute to a better understanding of molecular mechanisms of citral, and design of new drugs in the future.

journal_name

Int J Biol Macromol

authors

Alam MF,Varshney S,Khan MA,Laskar AA,Younus H

doi

10.1016/j.ijbiomac.2018.02.098

subject

Has Abstract

pub_date

2018-07-01 00:00:00

pages

300-308

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)34952-8

journal_volume

113

pub_type

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