Abstract:
:The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ-MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at different pHs, leading to the formation of LSZ-MAS microparticles. The higher preparation pH permitted greater adsorption affinity but a lower adsorption capacity of LSZ onto MAS. LSZ could interact with MAS via hydrogen bonds and electrostatic forces, resulting in the formation of intercalated nanocomposites. The particle size, %LSZ adsorbed, and LSZ release rate of LSZ-MAS microparticles increased when the LSZ-MAS ratio was increased. The secondary structure of LSZ bound onto the MAS layers in microparticles prepared at various pHs was altered compared with that of native LSZ. Moreover, the LSZ extracted from microparticles prepared at pH 4 showed an obvious change in the tertiary structure, leading to a decrease in the biological activity of the LSZ released. These findings suggested that LSZ can strongly interact with MAS to form microparticles that may potentially be used as delivery systems for sustained protein release.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kanjanakawinkul W,Medlicott NJ,Rades T,Puttipipatkhachorn S,Pongjanyakul Tdoi
10.1016/j.ijbiomac.2015.07.033subject
Has Abstractpub_date
2015-09-01 00:00:00pages
651-8eissn
0141-8130issn
1879-0003pii
S0141-8130(15)00504-8journal_volume
80pub_type
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