Abstract：
:The increase in industrial demand for peroxidases has necessitated the search for novel peroxidase with excellent industrial versatility. Raoultella ornithinolytica OKOH-1 is a new ligninolytic bacteria with peroxidase production potential. However, there is paucity of information on characterization of peroxidase from Raoultella species and its application potential in bioremediation. In this study, we characterized peroxidase from Raoultella ornithinolytica OKOH-1 (RaoPrx) for the first time using biochemical approach and bioinformatics; and as well investigated the dye-decolourization potential of the enzyme. RaoPrx oxidized various substrates, with pyrogallol giving the optimum activity. It had an optimum activity at pH 6 and was stable over a pH range of 5.0-7.0 with residual activity of above 40% after 120 min of incubation. The enzyme showed an optimum activity at 50 °C and was very stable at higher temperatures (50-70 °C) with residual activity of above 70% after 120 min. The enzyme was remarkably stable at 50 °C as it retained over 90% of its original activity after 120 min. The peroxidase activity was enhanced by Ag+, Cu2+, Zn2+and Fe2+, but was inhibited by Ca2+, Mg2+, Ba2+, Al3+, Co2+, NaN3 and EDTA. Furthermore, molecular characterization suggests RaoPrx as a novel dye-decolourizing peroxidase (DyP-type) family belonging to Class B, with estimated mo1ecular weight of 17.587 kDa and isoelectric point of 4.51, this is further confirmed by its remarkable dye-decolourizing activity on congo red and melanin in this study. This, therefore, indicates its application potential in textile dyes remediation and development of cosmetic agent.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Falade AO,Mabinya LV,Okoh AI,Nwodo UUdoi
10.1016/j.ijbiomac.2018.10.045subject
Has Abstractpub_date
2019-01-01 00:00:00pages
454-462eissn
0141-8130issn
1879-0003pii
S0141-8130(18)33718-8journal_volume
121pub_type
杂志文章abstract：:The mitochondrial genome (mitogenome) can provide information for phylogenetic analyses and evolutionary biology. We sequenced, annotated, and characterized the mitogenome of Euproctis similis. The complete mitogenome is 15,437bp in length, containing 13 protein-coding genes (PCGs), 22 transfer RNA genes, two ribosoma...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.07.033
更新日期：2017-12-01 00:00:00
abstract：:The present study aimed to investigate the biological functions of germinated M. oleifera seed proteins and to identify the identity of milk-clotting proteases. A total of 963 proteins were identified, and those with molecular weights between 10 and 30 kDa were most abundant. The identified proteins were mainly involv...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:From a supramolecular structure viewpoint, we concern how concurrent microwave and heat moisture treatment (M/HMT) tailors the pasting and digestion features of indica rice starch (IRS) and waxy rice starch (WRS), using combined analytical methods such as scanning electron microscopy and small-angle X-ray scattering. ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.189
更新日期：2019-08-15 00:00:00
abstract：:To study the properties and structure of aquatic silk, nest-spinning hydropsychid caddisfly (Stenopsyche marmorata) larva were collected from a Japanese river and the silk glands were removed from the larva by dissecting and dried on the glass plate at room temperature. The silk fibers were obtained by removing fibrou...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.10.003
更新日期：2010-01-01 00:00:00
abstract：:The purpose of this study was to gain insights into the hydrolysis characteristics of xylanase in producing xylo-oligosaccharides and to improve these by protein engineering. In this study, a hybrid enzyme (T-XynFM) was created from Talaromyces thermophilus F1208 xylanase (T-Xyn) by replacement of the N-terminal exten...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.12.050
更新日期：2017-03-01 00:00:00
abstract：:Nitric oxide (NO) is a promising therapeutic agent with antibacterial and wound-healing properties. However, the gaseous state and short half-life of NO necessitate a formulation that can control its storage and release. In this study, we developed NO-releasing films (CS/NO film) composed of chitosan (CS) and S-nitros...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.04.073
更新日期：2015-08-01 00:00:00
abstract：:Two types of the biological macromolecules poly(R-3-hydroxyalkanoates) have been identified: the high-molecular-weight microbial storage material (sPHA) and a short-chain variety, consisting of butyrate and valerate residues, complexed with other biomacromolecules such as calcium polyphosphate or proteins (cPHB/PHV). ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(99)00037-9
更新日期：1999-06-01 00:00:00
abstract：:Herein we represent a new discovery based on amine material called hexamethyltriethylene tetramine (HMTETA). We have observed that when an aqueous solution of Zn(NO3)·6H2O was added to aqueous solution of HMTETA followed by shaking for a time, the colorless solution was converted to milky color under the alkaline medi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.11.003
更新日期：2016-02-01 00:00:00
abstract：:Factor VIII (fVIII), a glycoprotein cofactor, plays a crucial role in the intrinsic blood coagulation pathway. As one of the most essential blood clotting factors known today, fVIII is the largest and most complex commercialized therapeutic protein used in the treatment of hemophilia A, an X-linked recessive disorder....
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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更新日期：2018-11-01 00:00:00
abstract：:Two exopolysaccharides, NPS and APS, were isolated from L. plantarum WLPL09 and purified by ion-exchange chromatography. The structural analyses showed that molecular weight of NPS and APS were 72.60 kDa and 33.22 kDa, respectively. NPS was mainly composed of mannose and glucose, in molar ratio of 85.35:14.65, while A...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.277
更新日期：2020-11-15 00:00:00
abstract：:A comprehensive study was conducted to elucidate physicochemical and structural properties of sago starches. Two sago starch granules were oval in shape with an average diameter of 34.41 μm and had C-type polymorph with a crystallinity of about 28.13%. The amylose and resistant starch (RS) contents of two sago starche...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.310
更新日期：2020-12-01 00:00:00
abstract：:Metallothioneins (MTs) are a family of Cys-rich, low molecular weight, cytoplasmic metal binding proteins. MTs are present in all eukaryotes as well as some prokaryotes. Plant MTs are divided into four types based on Cys distribution pattern in their amino acid sequences. In the present work, the gene encoding OsMTI-2...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.04.021
更新日期：2016-07-01 00:00:00
abstract：:In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced. The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties. The incorporation of sta...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.146
更新日期：2019-07-15 00:00:00
abstract：:Creatine kinase (CK) is a key enzyme for cellular energy metabolism, catalyzing the reversible phosphoryl transfer from phosphocreatine to ADP in vertebrates. CK contains a pair of highly conserved amino acids (H66 and D326) which might play an important role in sustaining the compact structure of CK by linking its N-...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.09.020
更新日期：2018-02-01 00:00:00
abstract：:The graft copolymerization of acrylonitrile (AN) onto methanol-soluble kraft lignin (ML) was achieved through a two-step process in which AN was first polymerized with an α,α'-azobisisobutyronitrile initiator, followed by radical coupling with activated ML. A carbon nanofiber material was obtained by electrospinning a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.10.022
更新日期：2016-01-01 00:00:00
abstract：:This work concerned the effects of amylose/amylopectin ratio on the structure and thermal stability of propionylated starches with high degree of substitution (DS). Four starches with different amylose content were used to obtain propionylated starches. Acylation partly disrupted granule morphology of native starches,...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.033
更新日期：2017-04-01 00:00:00
abstract：:This study aimed to evaluate the in vitro protective effect of topical treatment with a native sulfated polysaccharide of G. caudata (SP-Gc), hydrolyzed (H-SP-Gc), or desulfated (D-SP-Gc) polysaccharide of Gracilaria caudata in esophageal biopsies obtained from GERD patients. Biopsies were obtained from nonerosive ref...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.02.074
更新日期：2020-05-01 00:00:00
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journal_title：International journal of biological macromolecules
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更新日期：2020-02-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.02.098
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journal_title：International journal of biological macromolecules
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更新日期：2015-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-08-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2015-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2018-09-01 00:00:00
abstract：:For the first time, the effect of two novel designed pentapeptides on amyloid growth of human insulin using combined biophysical, microscopic, cell viability and computational approaches. Collective experimental data from ThT, ANS, and TEM demonstrate that in spite of having contrasting features, both peptides can eff...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-01-15 00:00:00
abstract：:Possible roles of two different Arginine (Arg; R) 213 and 337 on kinetic and structural stability of Photinus pyralis luciferase have been investigated using thermal and chemical denaturation studies. This enzyme is highly sensitive to protease digestion and temperature, which limits its fieldability, particularly for...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.09.007
更新日期：2013-01-01 00:00:00
abstract：:Bone tissue engineering holds great promise and clinical efficacy for the regeneration of bone defects. In this study, an amphoteric N,O-carboxymethyl chitosan (NOCC) and fucoidan (FD) were covalently cross-linked via an amidation reaction to synthesize NOCC/FD composite hydrogels. The hydrogels were lyophilized and t...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.179
更新日期：2018-12-01 00:00:00
abstract：:Thiothrix fructosivorans forms a microtube (sheath) that encloses a line of cells. This sheath is an assemblage of [→4)-GlcN-(1→4)-Glc-(1→]n with side chains of Rha4N-(1→3)-Fuc(1→ at position 3 of Glc. The sheath-forming polysaccharide (SFP) may have some substitutions but this is not yet confirmed. To investigate the...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.10.028
更新日期：2016-01-01 00:00:00
abstract：:In this study, the cells-free and cells-loaded chitosan hydrochloride-alginate (CHC-Alg) microcapsules were firstly fabricated with polyelectrolyte complexes via an orifice-polymerization method. Scanning electron microscope images showed that the CHC-Alg microcapsules had a typical shell-core structure and the model ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.09.034
更新日期：2016-12-01 00:00:00
abstract：:The aim of this study was to optimize the culture conditions for production, recovery and characterization of polyhydroxyalkanoate (PHA) from potential Bacillus cereus strain BNPI-92. Cost effective carbon sources such as crude oil (CO), rice bran (RB), sugar cane molasses (SCM) and wheat bran (WB) were evaluated for ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.138
更新日期：2020-08-01 00:00:00
