Abstract:
:A new hoop shaped three dimensional chitin was obtained successfully from the body segment of a diplopod species (Ommatoiulus sabulosus) by following the procedure decolorization, demineralization and deproteinization. Purity of the hoop shaped three-dimensional chitin was proved by FT-IR analysis and chitinase digestive test. The important bands for α-chitin such as 1654.2, 1619.7 and 1552cm-1 were found after FT-IR analysis. And the chitinase digestive test revealed the purity of chitin (with digestion rate of 94.7%). SEM analysis showed that the chitin surface consisted of highly porous structure with nanofibers. Thermal stability of the hoop shaped chitin was recorded quite high (DTGmax=383°C). The nitrogen, carbon and hydrogen contents of the hoop shaped chitin were determined as 6.81%, 46.23% and 6.43% respectively. And also degree of acetylation (DA) of the chitin indicated the purity with 95.85%. Chitin hoops-BSA interaction was conducted at different pH, protein concentration and contact time. This new type of three-dimensional chitin obtained from the diplopod body segments can find more effective applications in further studies comparing to the conventional dust forms.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Šatkauskienė I,Jarusevičiūtė S,Baublys V,Maheta M,Tubelytė Vdoi
10.1016/j.ijbiomac.2016.09.103subject
Has Abstractpub_date
2017-01-01 00:00:00pages
813-818issue
Pt Beissn
0141-8130issn
1879-0003pii
S0141-8130(16)31831-1journal_volume
94pub_type
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