Abstract：
:The equilibrium isotherms and adsorption kinetics of lysozyme (LZ) on epichlorohydrin (Epi) cross-linked alginate-guar gum (Alg-GG) matrix were studied. Adsorption kinetics followed a pseudo-first-order model while the equilibrium isotherm could be represented by the Freundlich equation. The maximal amount of LZ adsorbed onto this matrix was around 2.4mg per g of hydrated matrix at pH 7.00. The adsorption mechanism was associated to a simple diffusion process with a weak columbic interaction between LZ and the matrix. The presence of NaCl 0.3M induced a total displacement of the LZ from the matrix. Under this condition, the percentage of desorbed protein was 95%. Successive cycles of adsorption-washing-elution were performed and the results showed the reversibility of the process and the usefulness of the method for enzyme purification and separation. A last successful step was carried out for the purification of LZ from egg white as natural source. The model proved to be useful applied as a platform design in the isolation and purification of proteins.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Brassesco ME,Woitovich Valetti N,Picó Gdoi
10.1016/j.ijbiomac.2016.12.029subject
Has Abstractpub_date
2017-03-01 00:00:00pages
111-117eissn
0141-8130issn
1879-0003pii
S0141-8130(16)32864-1journal_volume
96pub_type
杂志文章abstract：:To screen effective anti-duck hepatitis A virus (DHAV) drugs, we applied STMP-STPP method to prepare phosphorylated Codonopsis pilosula polysaccharide (pCPPS), the phosphorylation-modified product of Codonopsis pilosula polysaccharide (CPPS). The IR spectrum and field emission scanning electron microscope (FE-SEM) wer...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2017-01-01 00:00:00
abstract：:Heparin is a member of the glycosaminoglycan (GAG) family composed of glucosamine and uronic acid units containing O-sulfo, N-acetyl and N-sulfo groups, which are alternating in the chain and linked by 1→4 manner. It is a naturally occurring anticoagulant that prevents the formation of clots and their growth within bl...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2017.03.036
更新日期：2017-06-01 00:00:00
abstract：:Alginate nanocomposite films incorporating sepiolite (Sep) modified with myrtle berries extract (MBE) rich in polyphenols were prepared by solution casting method. The effects of different extract concentrations on the film properties were determined by measuring physicochemical, mechanical and antioxidant properties ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-12-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2008.02.007
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abstract：:Noncompetitive carbon sources such as algae are unconventional and promising raw material for sustainable biofuel production. The capability of one marine bacterium, Saccharophagus degradans 2-40 to degrade red seaweed Gelidium amansii for production of polyhydroxyalkanoates (PHA) was evaluated in this study. S. degra...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.11.084
更新日期：2018-04-01 00:00:00
abstract：:Sequential glycopeptides [Thr(beta-D-galactose)-Ala-Ala]n, with n ranging from 2 to 7, as models of natural antifreeze glycoproteins were synthesized by the continuous flow, solid phase procedure. The conformational properties of these materials in solution were investigated by c.d. and 1H-n.m.r. spectroscopy. In aque...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(90)90080-t
更新日期：1990-02-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2008.01.007
更新日期：2008-05-01 00:00:00
abstract：:Chitosan is a biodegradable, non-toxic, and antimicrobial polymer. Chitosan films can be used as a dressing because they promote the healing of cutaneous ulcers. In this study, the mechanical, physical, and microbiological properties films of pure chitosan and films formulated with a glycerol-honey mixture were charac...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2011.04.002
更新日期：2011-07-01 00:00:00
abstract：:Poly(ε-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and m...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.036
更新日期：2019-03-15 00:00:00
abstract：:Oxidative stress is considered to involve cell death in severe pulmonary diseases like idiopathic pulmonary fibrosis (IPF). Polysaccharide FMP-1 from Morchella esculenta can exert significant antioxidant activity. However, its effects on alveolar epithelial cells remain unperceived. Herein, the effects of FMP-1 agains...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.08.148
更新日期：2018-12-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.04.041
更新日期：2016-10-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.218
更新日期：2020-07-15 00:00:00
abstract：:Bacillus licheniformis ATCC 9945A was cultivated in shake flasks using citrate (12 gl-1), glutamate (20 gl-1) and glycerol (80 gl-1) as carbon sources for cell growth and gamma-poly(glutamic acid) (gamma-PGA) production. The effect of the MnSO4 concentration in the medium over a range from 0.0 to 615 microns was studi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(95)98153-p
更新日期：1995-10-01 00:00:00
abstract：:This study was conducted to confirm the effects of salinity stress on bioactive compounds and antioxidant activity of wheat microgreen extract. The microgreens were cultivated for 8 days in organic media with different concentrations of Na [0 (control), 12.5, 25, 50, and 100 mM from sodium chloride] which was containe...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2019-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.12.023
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.12.139
更新日期：2018-05-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.107
更新日期：2018-04-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2019.12.181
更新日期：2020-03-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.266
更新日期：2020-11-15 00:00:00
abstract：:In this study, the adjuvant activity of sulfated glucan from saccharomyces cerevisiae (SGSC) was investigated. sGSC had molecular weight of 12.9kDa and degrees of sulfation of 0.16. The effects of SGSC on the splenic lymphocyte cells of mice were measured in vitro. The results showed that SGSC could significantly prom...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.06.057
更新日期：2016-12-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.08.054
更新日期：2016-12-01 00:00:00
abstract：:The isolation process of alkali lignin (AL) from the valonea of Quercus variabilis Blume was optimized (liquid/solid ratio, 12.21; isolation time, 4.21h; isolation temperature, 42.21°C; and alkali concentration, 0.85mol/L) using the response surface method (RSM), with the highest isolation rate obtained being 22.67%. ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.008
更新日期：2017-04-01 00:00:00
abstract：:Inonotus obliquus polysaccharides (IOPs) are a potential drug for the prevention and treatment of cancer, cardiopathy, diabetes, AIDs, pancreatitis and other diseases. In this study, we found that IOP can act as a broad-spectrum antiviral drug against feline viruses in the in vitro experiment. Using cell models of fel...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.11.054
更新日期：2017-02-01 00:00:00
abstract：:Novel, biocompatible, multifunctional, injectable genipin crosslinked collagen/chitosan/lysine-modified hyaluronic acid based hydrogels (ColChHAmod) were prepared in a facile, one-step procedure. The novelty of the current approach lies in the functionalization of hyaluronic acid (HA) with primary amine groups by lysi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.052
更新日期：2020-07-15 00:00:00
abstract：:Opuntia is a traditional plant from China with medicinal applications. In this experiment, polysaccharides from Opuntia Milpa Alta (MAPs) were analyzed using gas chromatograph-mass spectrometer (GC-MS) method and result showed that MAPs contained mannose (6.37%), rhamnose (14.94%), xylose (1.99%), arabinose (24.07%), ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2011.06.031
更新日期：2011-11-01 00:00:00
