Abstract:
:The interactions of fish gelatine (FG) with hyaluronic acid (HA) are studied in an aqueous environment at 25°C by turbidimetric titration, confocal scanning laser microscopy, dynamic light scattering, zeta potentiometry, spectrophotometry with methylene blue, and construction of state diagrams. FG forms soluble complexes with HA above a boundary pH (pHφ1), where both biopolymers are net-negatively charged, but develop insoluble complexes as liquid-state complex coacervates below pHφ1, where the two biopolymers are oppositely charged. The insoluble complexes are continuously aggregated with further acid titration, followed by immediate visible phase-separation when another boundary pH (pHp) is reached. The complex formation is mainly driven by electrostatic attractions rather than hydrogen bonding or hydrophobic interactions. The complex formation is promoted by increasing FG-to-HA weight ratio or total biopolymer concentration, or at a low ionic strength, but significantly suppressed in the presence of high ionic strength.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Razzak MA,Kim M,Kim HJ,Park YC,Chung Ddoi
10.1016/j.ijbiomac.2017.04.083subject
Has Abstractpub_date
2017-09-01 00:00:00pages
885-892eissn
0141-8130issn
1879-0003pii
S0141-8130(16)33054-9journal_volume
102pub_type
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