Abstract:
:The present research investigates on task-specific deep eutectic solvents (TDES) based aqueous two-phase extraction and purification of immunoglobulins by chromatography from quail egg. The synthesis of TDES was accomplished with quaternary ammonium salt as hydrogen bond acceptor (HBA) and glycerol as hydrogen bond donor (HBD). Aqueous two-phase (ATPS) formation of TDESs with various salts was established and phase partitioning ability was evaluated using standard bovine immunoglobulin. Ultrasound-assisted liquid-liquid microextraction (UA-LLME) was performed with ATPS of better partitioning ability for quail egg yolk. Optimization of influential variables with response surface methodology was accomplished and maximum yield was achieved for 85% (v/v) of NADES for feed of 18 μg/ml of egg yolk isolate with ultrasound temperature of 35 °C and 12 min contact time respectively. The quantification of recovery was accomplished by gel filtration process with the determination of retention time, volume and resolution of separation using marker protein. Ultrapure immunoglobulin-Y (IgY) is achieved with anion exchange chromatography and yield was calculated. The results revealed that the selective concentration of immunoglobulin-Y from quail egg yolk could be performed using environment-friendly TDES based UA-LLME coupled with chromatography.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Balaraman HB,Rathnasamy SKdoi
10.1016/j.ijbiomac.2019.12.242subject
Has Abstractpub_date
2020-03-01 00:00:00pages
253-262eissn
0141-8130issn
1879-0003pii
S0141-8130(19)38565-4journal_volume
146pub_type
杂志文章abstract::A novel flame retardant (FR), the ammonium salt of citrulline-penta (methylphosphonic acid) (ACPMPA) based on L-citrulline was synthesized, and its structure was characterized by 13C, 1H, and 31P nuclear magnetic resonance (NMR) spectroscopy. The ACPMPA flame retardant molecule contains five ammonium salts of phosphor...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.11.022
更新日期:2021-01-01 00:00:00
abstract::Pullulan (PU) is an exo-polysaccharide used in the food and pharmaceutical industries. However, the use of PU in different industries is limited due to its highly hydrophilic nature and consequently weakness in surface properties which can be remedied by its chemical modification with octenyl succinic anhydride (OSA)....
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.158
更新日期:2020-12-01 00:00:00
abstract::Amylopectin is a highly branched starch component built up of a large number of clustered α-D-glucose chains. A single C-chain possesses the reducing end and carries the rest of the macromolecule. The aim of this study was to investigate the interconnection of clusters and domains (groups of clusters) in barley amylop...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2012.12.032
更新日期:2013-04-01 00:00:00
abstract::In the present study, the influence of different concentrations of three fatty acids (palmitic, stearic, and oleic acids) on improving the physicochemical, barrier, mechanical, thermal and surface properties of Salvia macrosiphon seed gum (SSG) edible films was investigated. The results revealed that the selected fatt...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.034
更新日期:2020-11-01 00:00:00
abstract::A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities were 60%, 58%, 41% and 14%, respectively, very likely due to casein d...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.12.140
更新日期:2020-02-01 00:00:00
abstract::Antioxidant effects of lentinan on LPS induced inflammatory response in intestine of juvenile taimen were evaluated, and its prebiotic-like efficacy on intestinal microbiota was also investigated. The results showed that LPS decreased the activities of antioxidant enzymes and increased the expression levels of inflamm...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.121
更新日期:2019-01-01 00:00:00
abstract::We report the preparation of both control chitosan and magnetic chitosan beads as biosorbents using chitosan as matrix and magnetite (Fe3O4) nanoparticles as reinforcement followed by detailed advanced characterization. The batch trials were performed to study the adsorption kinetics of biosorbents by removing As(III)...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.06.287
更新日期:2020-11-15 00:00:00
abstract::In this research, a comparative proteomics approach was conducted to understand the physiological processes behind the sulforaphane formation in whitetop seedlings in response to exogenous glucose. Initially, 5-day-old whitetop seedlings were elicited by different concentrations (0, 166, 250, 277, 360 mM) of glucose f...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.09.016
更新日期:2018-12-01 00:00:00
abstract::Conjugation of DNA with testosterone and it aliphatic dimer (alip) and aromatic dimer (arom) was investigated in aqueous solution at pH 7.4. Multiple spectroscopic methods, transmission electron microscopy (TEM) and molecular modeling were used to characterize steroid-DNA binding and DNA morphology. Spectroscopic anal...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2016.09.090
更新日期:2017-02-01 00:00:00
abstract::Among the various types of polyhydroxyalkanoate (PHA), poly[(R)-3-hydroxybutyrate-co-(R)-3-hydroxyhexanoate] [P(3HB-co-3HHx)] has a high potential to serve as commercial bioplastic due to its striking resemblance to petroleum-based plastics. In this study, five different genotypes of Cupriavidusnecator transformants h...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.05.064
更新日期:2020-09-15 00:00:00
abstract::The current study involves development of novel tamarind seed gum - alginate complex microspheres for sustained release of dalfampridine by using Central Composite design in combination with response surface methodology. Polymer ratio (A), agitation speed (B) and concentration of CaCl2 (C) were selected as independent...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.11.203
更新日期:2020-02-01 00:00:00
abstract::In order to investigate the effects of different preparation methods on Semen coicis resistant starch, the structural properties and prebiotic effects of autoclaving treatment-purified S. coicis resistant starch (GP-SRS3), ultrasonic-autoclaving treatment-purified S. coicis resistant starch (UP-SRS3), enzyme-autoclavi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.07.091
更新日期:2017-12-01 00:00:00
abstract::Laccase was immobilized using a combinatorial strategy of cross-linking and entrapping in mesoporous silica to prepare entrapped enzyme species including simply adsorbed, entrapped cross-linked enzyme (E-CLE) and entrapped cross-linked enzyme aggregate (E-CLEA) to explore their potential in phenol removal. Parameters ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.10.147
更新日期:2019-02-01 00:00:00
abstract::Dinoflagellates are the dominant source of bioluminescence in coastal waters. The luminescence reaction involves the oxidation of luciferin by a luciferase enzyme, which only takes place at low pH. The pH-dependence has previously been linked to four conserved histidines. It has been suggested that their protonation m...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.071
更新日期:2020-12-01 00:00:00
abstract::Acetaminophen (APAP)-induced acute kidney injury (AKI) has turned into a typical reason for clinic obtained renal disappointment. In any case, the advancement of prophylaxis procedures and endorsed treatments for APAP-AKI is restricted. Sika deer antler protein (SDAPR) has a protective effect on drug-induced AKI. The ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.08.164
更新日期:2019-12-01 00:00:00
abstract::An arabinogalactan (ICPA) was isolated from the water extract of Ixeris chinensis (Thunb.) Nakai. ICPA was mainly composed of galactose and arabinose with minor amount of glucose. The molecular weight of ICPA was 58.1 kDa. Structural analysis by methylation and NMR spectroscopy indicated that ICPA contained α-D-Glcp(1...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.09.158
更新日期:2020-01-15 00:00:00
abstract::Polymer microbeads (MBs) for scrubbing additives have generally been prepared from non-biodegradable synthetic polymers. The worldwide pollution of the marine ecosystem by microplastics urgently demands novel environment-friendly MBs. In this study, Ca-alginate MBs were fabricated by electrospraying an aqueous alginat...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.04.058
更新日期:2019-07-15 00:00:00
abstract::The aim of this work is to study the suitability of different soda cereal straw pulps (wheat, barley, corn and oats) to produce lignocellulose nanofibers (LCNF). For the study of the influence of the composition and physical characteristics, the pulps were characterized, observing that the physical characteristics do ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.05.156
更新日期:2017-10-01 00:00:00
abstract::In recent years, the unparalleled and functional properties of essential oils have been extensively reported, but the sensitivity of essential oils to environmental factors and their poor aqueous solubility have limited their applications in industries. Hence, we encapsulated CEO in chitosan nanoparticles by an emulsi...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.08.010
更新日期:2015-11-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is considered as one of the most aggressive cancer worldwide. In Egypt, the prevalence of HCC is increasing during last years. Recently, drug-loaded microparticles were used to improve the efficiency of various medical treatments. This study is designed to evaluate the anticancer potenti...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2014.05.031
更新日期:2014-08-01 00:00:00
abstract::The purpose of this study is to clarify that the amino acid residues (Asp62 and Arg193) are responsible for the activity and stability of arginine kinase (AK). The amino acid residues Asp62 (D62) and Arg193 (R193) are strictly conserved in monomeric AKs and form an ion pair in the transition state analogue complex. In...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2011.05.022
更新日期:2011-10-01 00:00:00
abstract::The aim of this study was to improve the physicochemical stability of neohesperidin (NH) using nanoliposomal encapsulation in association with surface decoration strategy employing chitosan (CH) and pectin (P). Different nanoliposomal systems, i.e. NH-loaded nanoliposome (NH-NL), CH-coated NH-NL (CH-NH-NL), and P-coat...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2020.08.174
更新日期:2020-12-01 00:00:00
abstract::Our previous study suggested that extrusion can enhance the soluble dietary fiber (SDF) and improve the physicochemical properties of fiber-rich orange pomace (OP). The aim of this study was to determine the predictive indices for the hypoglycemic, hypocholesterolemic, and fermentation capacities of extruded orange po...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.11.249
更新日期:2019-03-01 00:00:00
abstract::In vitro fibrillation of hen egg white lysozyme (HEWL) causes complete reduction of Cu(II) to Cu(I) at pH 7. Here in the present article, we have shown the presence of both Cu(II) and Cu(I) at pH 11 during fibrillation of HEWL using electron paramagnetic resonance and Raman spectroscopy. Our results suggest the existe...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.02.014
更新日期:2015-05-01 00:00:00
abstract::A novel protein (J2-C4) with antioxidant and antibacterial activities was purified from the edible portion of Arca inflata. The purity of J2-C4 was measured to be 97.62% by RP-HPLC analysis, and the molecular weight was 20,537.0 Da by ESI-MS/MS. The isoelectric point of J2-C4 was determined to be 5.18 by IEF-PAGE. Sec...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.05.008
更新日期:2018-09-01 00:00:00
abstract::From a supramolecular structure viewpoint, we concern how concurrent microwave and heat moisture treatment (M/HMT) tailors the pasting and digestion features of indica rice starch (IRS) and waxy rice starch (WRS), using combined analytical methods such as scanning electron microscopy and small-angle X-ray scattering. ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.05.189
更新日期:2019-08-15 00:00:00
abstract::The corynemycolyltransferase proteins were identified from Corynebacterium glutamicum and Corynebacterium efficiens genomes using computational tools available in the public domain. Three-dimensional models were constructed for corynemycolyltransferases based on the crystal structures of related mycolyltransferases in...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2004.03.008
更新日期:2004-06-01 00:00:00
abstract::Corn starch was melt-blended with glycerol (70/30) to prepare thermoplastic starch (TPS) at 140 °C for 10 min. The TPS was melt-blended with polyethylene grafted with maleic anhydride (PE-MAH) and carboxymethyl cellulose (CMC) to improve the properties of the TPS. Phase determination analysis and SEM images demonstrat...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.08.076
更新日期:2018-12-01 00:00:00
abstract::The objectives of this study were to investigate the adsorption behavior of lysozyme (LSZ) onto magnesium aluminum silicate (MAS) at various pHs and to characterize the LSZ-MAS microparticles obtained from the molecular interaction between LSZ and MAS. The results showed that LSZ could be bound onto the MAS layers at ...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2015.07.033
更新日期:2015-09-01 00:00:00
abstract::Cytochrome P450 monooxygenases (CYP450s) and their auxiliary cytochrome P450 reductases (CPRs) are important and commonly involved in the biosynthesis of camptothecin (CPT). To better understand the possible functions of CPRs in planta, we first isolated two CaCPRs genes from Camptotheca acuminata. Sequence analysis r...
journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.07.141
更新日期:2019-10-01 00:00:00