Abstract:
:The present study was undertaken to characterize the extracellular thermostable serine alkaline proteases from newly actinomycete strain Micromonospora chaiyaphumensis S103 and to describe their evaluation in commercial detergents and shrimp waste deproteinization. This proteolytic crude extract was active and stable in alkaline solution. It was extremely stable in the pH range of 5.0-12.0. The optimum pH and temperature were 8.0 and 70°C, respectively, using casein as a substrate. The thermoactivity and thermostability of proteases were enhanced by the addition of 5mM Ca2+. Proteases from S103 were also used for shrimp wastes deproteinization in the process of chitin preparation. The percent of protein removal after 3h hydrolysis at 45°C with an enzyme/substrate ratio of 20U/mg had reached 93%. Furthermore, S103 crude enzyme was stable towards several organic solvents and retained 100% of its original activity after 90days of incubation in the presence of methanol, hexane, acetone, and DMSO. These properties make S103 proteases an ideal choice for application in detergent formulations, chitin production, and enzymatic peptide synthesis.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Mhamdi S,Ktari N,Hajji S,Nasri M,Sellami Kamoun Adoi
10.1016/j.ijbiomac.2016.10.036subject
Has Abstractpub_date
2017-01-01 00:00:00pages
415-422issue
Pt Aeissn
0141-8130issn
1879-0003pii
S0141-8130(16)30790-5journal_volume
94pub_type
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