Abstract：
:Electrospinning of chitin/silk fibroin (SF) blend solutions in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) was investigated to fabricate a biomimetic nanostructured scaffolds for tissue engineering. The morphology of the electrospun chitin/SF blend nanofibers was investigated with a field emission scanning electron microscope (FE-SEM). The average diameters of chitin/SF blend fibers decreased from 920 to 340nm, with the increase of chitin content in blend compositions. The miscibility of chitin/SF blend fibers was examined by solution viscosity measurement. The chitin and SF were immiscible in the as-spun nanofibrous structure. The dimensional stability of chitin/SF blend nanofibers, with or without water vapor after-treatment, was conducted by immersing in water. As-spun SF-rich blend nanofibrous matrices were lost their fibrous structure after the water immersion for 24h, and then changed into membrane-like structure. On the contrary, nanofibrous structures of water vapor-treated SF-rich blends were almost maintained. To assay the cytocompatibility and cell behavior on the chitin/SF blend nanofibrous scaffolds, cell attachment and spreading of normal human epidermal keratinocyte and fibroblasts seeded on the scaffolds were studied. Our results indicate that chitin/SF blend nanofibrous matrix, particularly the one that contained 75% chitin and 25% SF, could be a potential candidate for tissue engineering scaffolds because it has both biomimetic three-dimensional structure and an excellent cell attachment and spreading for NHEK and NHEF.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Park KE,Jung SY,Lee SJ,Min BM,Park WHdoi
10.1016/j.ijbiomac.2006.03.003keywords:
subject
Has Abstractpub_date
2006-05-30 00:00:00pages
165-73issue
3-5eissn
0141-8130issn
1879-0003pii
S0141-8130(06)00089-4journal_volume
38pub_type
杂志文章abstract：:Our laboratory has developed a technique to reconstruct in vitro tissue from human cells using the self-assembly tissue-engineering method, which utilizes the ability of fibroblasts to deposit the matrix they secrete. The time necessary for tissue construction, several weeks, is a drawback for many clinical uses. We h...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:Brachyura is one of the most species rich and highly derived groups among extant crustaceans, with over 7250 known species. However, brachyuran phylogeny remains controversial and requires further study. Here, we combined 103 brachyuran mitogenomes from GenBank with 10 new mitogenomes to describe gene rearrangement pa...
journal_title：International journal of biological macromolecules
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abstract：:Porous scaffolds composed of alginate (AG) and chitosan (CS) were fabricated by combining the formation of polyelectrolyte complex (PEC) with freeze-drying. The AG scaffold was used as a framework with uniformly distributed and interconnected pore structure. The chitosan or chitosan/hydroxyapatite (CS/HA) composite so...
journal_title：International journal of biological macromolecules
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更新日期：2010-03-01 00:00:00
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journal_title：International journal of biological macromolecules
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abstract：:The cDNA of the β-1,4-endoxylanase of Aspergillus niger US368 was cloned and expressed in Pichia pastoris under the constitutive GAP promoter. The maximum activity obtained was 41 U mL(-1), which was about 3-fold higher than that obtained with the native species. The purified enzyme showed a specific activity of 910 U...
journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
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abstract：:Waxy maize starch was irradiated under different doses of radiation (2-30 kGy), and starch physicochemical properties were analysed. Films were subsequently produced from native and irradiated waxy maize starches and their properties were tested. The starch molecular weight markedly decreased with increasing irradiati...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:A novel composite hydrogel was prepared as a dual drug delivery carrier. Poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) microparticles were prepared to encapsulate simultaneously ketoprofen and mupirocin, as hydrophobic drug models. These microparticles were embedded in a physically crosslinked hydrogel of κ-carragee...
journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites. However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application. Herein, a facile method was proposed to fabricate polydopa...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2015-09-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.07.013
更新日期：2015-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.11.102
更新日期：2017-02-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.021
更新日期：2019-06-01 00:00:00
abstract：:Bacteriocins are bioactive antimicrobial peptides synthesized in the ribosome of numerous bacteria and released extracellularly. Bacteriocins have the ability to kill or inhibit the growth of prokaryotes and could potentially be useful against pathogens and antibiotic-resistant strains of bacteria. The antimicrobial m...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2018.05.233
更新日期：2018-10-01 00:00:00
abstract：:Stabilization of type I rat tail tendon (RTT) collagen by various aldehydes, viz. formaldehyde, gluteraldehyde, glyoxal and crotanaldehyde was studied to understand the effect of each on the thermal, enzymatic and conformational stability of collagen. The aldehydes have been found to increase the heat stability of rat...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2004.05.004
更新日期：2004-08-01 00:00:00
abstract：:Biodegradable flocculants of Gum ghatti (Gg) with acrylamide (AAm) were prepared through graft co-polymerization technique using potassium persulphate (KPS)-ascorbic acid (ABC) redox pair as initiator and N,N'-methylene-bis-acrylamide (MBA) as a crosslinker. Gg-cl-poly(AAm) was found to exhibit pH and temperature resp...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.03.045
更新日期：2013-07-01 00:00:00
abstract：:The purpose of this study was to investigate the immune and antioxidant activities of Glycyrrhiza glabra polysaccharides (GGP) in rats fed high-fat diet. The experiment was performed on four groups of growing Kunming mice. The results of the experiment showed a statistically significant decrease in serum antioxidant e...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2009.04.001
更新日期：2009-07-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2018-07-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(00)00169-0
更新日期：2001-03-14 00:00:00
abstract：:Chitin/alginate (CA) blend membranes were successfully prepared from 8 wt% NaOH/4 wt% urea aqueous solution by coagulating with 0.5M CaCl(2) aqueous solution containing 5 wt% HCl. Morphology, mechanical properties, thermal stability, and antimicrobial effects of the membranes were investigated by scanning electron mic...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2012-12-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2005-12-15 00:00:00
abstract：:The aim of the present study was to evaluate the anti-inflammatory ability of novel indigenous probiotic Lactobacillus fermentum MCC 2759, L. fermentum MCC 2760 and Lactobacillus delbrueckii MCC 2775 in a carrageenan induced acute inflammatory paw edema model. Probiotic cultures were administered to male Wistar rats v...
journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
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更新日期：2018-06-01 00:00:00
abstract：:Gene therapy is a promising alternative that ensures effective treatment and cure for cancer. Here, we report graphene-reinforced chitosan (CS) construct based non-viral vector for tumor-targeted gene therapy. The therapeutic gene, pDNA-TNF-α, was loaded on to chitosan-carboxylated graphene oxide (CS-CGO) construct vi...
journal_title：International journal of biological macromolecules
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更新日期：2019-04-15 00:00:00
abstract：:Identification of biomarker proteins in the retina and retinal pigment epithelium (RPE) under oxidative stress may imply new insights into signaling mechanisms of retinal degeneration at the molecular level. Proteomic data from an in vivo mice model in constant light and an in vitro oxidative stress model are compared...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2010-12-01 00:00:00
abstract：:Three 4-(1H-imidazo[4,5-f]-1,10-phenanthrolin-2-yl)phenol derivatives-based platinum (II) complexes have been synthesized, and their G-quadruplex DNAs-binding interactions, telomerase inhibition, antiproliferative activity, and cell cycle arrest were studied. Three complexes show the preference for stabilizing h-telo,...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2013-04-01 00:00:00
abstract：:To understand the mechanism through which cardaba banana starch is hydrolysed, the starch digestion kinetics of native and modified cardaba banana starch samples of Nigeria origin were investigated using an in vitro procedure. The digestion kinetics of the starch samples revealed the samples exhibited a biphasic diges...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-07-01 00:00:00
abstract：:Chitosan (300 kDa) was degraded by cellulase to chitosans with molecular weights (MWs) of 156, 72, 7.1, and 3.3 kDa and a chitooligosaccharide mixture (COS). Effects of these on NO secretion, cytokine production, and mitogen-activated protein kinase pathways in lipopolysaccharide (LPS)-induced murine RAW 264.7 macroph...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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