当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > A non-naturally-occurring P(3HB-co-3HAMCL) is produced by recombinant Pseudomonas sp. from an unrelated carbon source.

A non-naturally-occurring P(3HB-co-3HAMCL) is produced by recombinant Pseudomonas sp. from an unrelated carbon source.

Abstract：

:Pseudomonas sp. PHA- was used as host for PHA biosynthesis genes from Aeromonas sp. to produce 3HB-co-3HAMCL from glucose with no supply of co-substrates. A non-naturally-occurring PHA composed mainly of 3HB, 3HHx and 3HD (3HO, 3HDdΔ5 and 3HDd monomers were detected in smaller amounts) was obtained. The polymer was extracted using two different solvents (acetone and chloroform) and subject to the following characterization tests: FTIR, DSC, TGA and GPC. The latter suggests a block copolymer since a single and narrow elution peak was observed for each sample. The DSC results ruled out the possibility of a random copolymer and agrees with a single copolymer composed of two blocks: one with the typical composition of PHAMCL produced by Pseudomonas and another containing 3HB and 3HHx with a high 3HHx molar fraction. Thus, this study increases the perspectives of P(3HB-co-3HAMCL) production from carbohydrates as the sole carbon source.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Cespedes LG,Nahat RATPS,Mendonça TT,Tavares RR,Oliveira-Filho ER,Silva LF,Taciro MK,Sánchez RJ,Gomez JGC

doi

10.1016/j.ijbiomac.2018.03.051

subject

Has Abstract

pub_date

2018-07-15 00:00:00

pages

512-519

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)34152-1

journal_volume

114

pub_type

杂志文章

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