Abstract：
:The macromolecular crowding progressively has been gaining prominence in recent years as it acts as a sword with double-edge on protein stability and folding, i.e., showing assorted results of having both stabilizing and destabilizing effects. We studied the effects of different concentrations of polyethylene glycol (PEG-10) on structure and stability of myoglobin. The tertiary structure of myoglobin was found to be perturbed in the presence of polyethylene glycol, however there was insignificant change in the secondary structure. It was observed that polyethylene glycol induces molten globule state in myoglobin, where the intermediate state holds hydrophobic patches and larger hydrodynamic volume as compared to the native protein. In addition, isothermal titration calorimetry (ITC) showed strong binding between myoglobin and polyethylene glycol, at the physiological pH. We hypothesize that polyethylene glycol induces molten globule conformation in myoglobin by interacting with heme group of myoglobin. We caution that the binding of protein with crowder and other soft interactions need to be gravely well thought-out when studying macromolecular crowding. In our case, destabilizing protein-crowder interactions could compete and overcome the stabilizing exclusion volume effect.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Parray ZA,Shahid S,Ahmad F,Hassan MI,Islam Adoi
10.1016/j.ijbiomac.2017.02.084subject
Has Abstractpub_date
2017-06-01 00:00:00pages
241-248eissn
0141-8130issn
1879-0003pii
S0141-8130(16)33036-7journal_volume
99pub_type
杂志文章abstract：:Thin films of chitosan (CS) coated on glass substrates were used as support for the immobilization of nanostructures TiO2/ZnS via a mild chemical process followed by a hydrothermal treatment at 90 °C. The preparation route involved the coating of the CS film on the glass substrate followed by the deposition of a thin ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.05.141
更新日期：2018-09-01 00:00:00
abstract：:A gene, encoding a new alginate lyase AlgNJU-03, was cloned from marine bacteria Vibrio sp. NJU-03. The recombinant alginate lyase was characterized followed by being purified by NTA-Ni Sepharose. It exhibited the highest activity (6468.99U/mg) at pH 7.0 and 30°C. Interestingly, AlgNJU-03 possessed broader substrate s...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2018-03-01 00:00:00
abstract：:Lectins are proteins that have as one of their main characteristics recognizing and reversibly binding to carbohydrates. In this work, it was possible to purify and characterize a lectin from Parkia panurensis (Leguminosae family; Mimosoideae subfamily) seeds by a combination of the techniques: protein precipitation, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-02-15 00:00:00
abstract：:The fruiting body of Hericium erinaceus has been used to treat digestive system disorder-related diseases for over 2000 years in China. A novel polysaccharide, HEFP-2b, was obtained from H. erinaceus fruiting bodies. Physical and chemical analysis showed that HEFP-2b consisted of fucose, galactose, glucose, and mannos...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:In the present study, high-methoxyl pectin (HMP) was extracted from Hylocereus polyrhizus peel's using physico-chemical process. In addition, the hypolipidemic activity of HMP was investigated at different concentration and time corresponding to its adsorption ability. FTIR and contact angle analysis were used to dete...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.143
更新日期：2019-08-01 00:00:00
abstract：:The purpose of this study was to develop a new therapeutic approach for atorvastatin (ATV) adopting nanostructured polymeric micelles for its controlled delivery to the cancer cells. Amphiphilic block copolymers of stearyl chitosan (SC) and sulfated stearyl chitosan (S-SC) that could self assemble to form polymeric mi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2012-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.079
更新日期：2020-12-01 00:00:00
abstract：:The contribution of chitosan/nanocellulose (CS-NC) to the enzymatic activity of Candida rugosa lipase covalently bound on the surface of CS-NC (CRL/CS-NC) was investigated. Cellulosic material from oil palm frond leaves (OPFL) were bleached, alkaline treated and acid hydrolyzed to obtain the purified NC and used as na...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.095
更新日期：2018-07-15 00:00:00
abstract：:Co-aggregation plays an important role in processing protein-rich food materials under heterogeneous conditions. The main cause of co-aggregation is an electrostatic attraction between oppositely charged molecules. This study investigated thermal aggregation of β-lactoglobulin (BLG) (pI=5.1) and lysozyme (LYZ) (pI=10....
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2018-02-01 00:00:00
abstract：:Existing therapies yield low drug encapsulation or accumulation in the lungs, hence the site-specific drug delivery remains the challenge for tuberculosis. Lately, dry powder inhalers (DPIs) are showing promising drug deposition in the deeper lung tissues. Biocompatible polymers with the ability to naturally recognize...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.192
更新日期：2020-12-15 00:00:00
abstract：:In this study functional film enrichment with antioxidant of bamboo leaves (AOB) was prepared based on carboxymethyl cellulose-gelatin (CMC-Gel) mixture using casting method. Microstructures of the films were performed on atomic force microscope, Fourier transform infrared spectrometer, and X-ray diffraction (XRD). Th...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.286
更新日期：2020-12-01 00:00:00
abstract：BACKGROUND:Advanced glycation end products (AGEs) have shown to possess antigenicity. This study analyzes the detrimental effect of non-enzymatic glycation on human serum albumin (HSA) leading to the production of antibodies. METHODS:HSA (20 μM) incubated with d-glucose formed AGEs confirmed by scanning electron micro...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.07.033
更新日期：2018-10-15 00:00:00
abstract：:An acidic homogeneous polysaccharide (HD-PS-1) was purified from Hedyotis diffusa (Willd.) Roxb. HD-PS-1 possessed a backbone chain of →[4)-β-Glcp-3-OAc-(1]6→[6)-β-Manp-(1]2→6)-α-Galp-(1→[4)-α-Galp-(1]2→, with three branches of β-Manp-(1→3)-β-GlcpA, α-Rhap-(1→3)-α-Rhap and α-Galp attached to the backbone chain at O-4 ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.11.132
更新日期：2020-07-15 00:00:00
abstract：:Development of bio-nanoreinforcement is gaining momentum worldwide nowadays since it contributes much to the green materials era. This paper presents a proposal for reusing the agro waste to produce cellulose nanofibres in an ecofriendly way. The present work aims to isolate cellulose nanofibres (CNF) from pineapple l...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.134
更新日期：2019-06-15 00:00:00
abstract：:Sodium alginate, a naturally occurring macromolecule, in its granular form and without any post-modification was found to be an efficient and recoverable bifunctional heterogeneous organocatalyst for the domino synthesis of various 2-amino-3-cyano-4H-pyran annulated derivatives through three-component condensation of ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.01.080
更新日期：2016-06-01 00:00:00
abstract：:Removals of tetracycline and photocatalytic degradation of phenol by Fe3O4/coffee residue (MCC) were investigated. Brunauer-Emmett-Teller (BET), vibrating sample magnetometer (VSM) and Boehm titration were employed to characterize MCC. Artificial neural network (ANN) model was developed to predict the tetracycline (TC...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.08.054
更新日期：2016-09-01 00:00:00
abstract：:A thermostable superoxide dismutase from thermophilic bacterium Anoxybacillus gonensis KA 55 MTCC 12684 which was isolated from Manikaran hotspring of Himachal Pradesh was purified to apparent homogeneity by fractional ammonium sulphate precipitation and anion exchange chromatography. A purification factor of 33.1-fol...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.06.031
更新日期：2018-10-01 00:00:00
abstract：:The formation of heteroprotein coacervates obtained by the interaction of ovalbumin (Ova) and lysozyme (Lys) was investigated using turbidimetric analysis and the zeta potential at different protein ratios, pH values and concentrations of NaCl. The complexes were formed over a wide pH range with a 1:1 (Ova:Lys) ratio ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.08.132
更新日期：2018-01-01 00:00:00
abstract：:Three anionic chitosan surfactant with different hydrophobic tails labeled Chitosan-R8, Chitosan-R12 and Chitosan-R16 were prepared and their surface behavior in aqueous solution was determined by surface tension measurements at three different temperatures 20, 40 and 60 °C. The affinity of the synthesized anionic chi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.04.184
更新日期：2020-08-15 00:00:00
abstract：:IP3 is a ubiquitous second messenger in eukaryotic cells that triggers Ca2+-release from intracellular stores. IP3 binds to intracellular IP3-receptor (IP3R) and induces conformational change within the ligand-binding domain which regulates Ca2+-release; hence, both IP3 and IP3R are key components of the signal transd...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.117
更新日期：2019-03-01 00:00:00
abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published in Int. J. Biol. Macromol, doi:10.1016/j.ijbiomac.2008.07.012. The duplicate article has therefore been withdrawn. ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2008.08.002
更新日期：2008-08-19 00:00:00
abstract：:Advanced local delivery systems are needed as adjunctive treatments for severe injuries with high infection rates, such as open fractures. Chitosan systems have been investigated as antimicrobial local delivery systems for orthopaedic infection but possess mismatches between elution and degradation properties. Derivat...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.075
更新日期：2019-07-15 00:00:00
abstract：:Sphallerocarpus gracilis is a medicinal and edible plant in China with high nutritional value. We examined the structure and bioactivity of a new polysaccharide fraction purified from S. gracilis named SGP-1. This polysaccharide possessed an average molecular weight of 4.916 × 105 and was primarily composed of arabino...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.208
更新日期：2019-05-15 00:00:00
abstract：:There are different clotting factors present in blood, carries the clotting cascade and excessive bleeding may cause a deficiency in the clotting Diagnosis of this deficiency in clotting drastically reduces the potential fatality. For enabling a sensor to detect the clotting factors, suitable probes such as antibody a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2018.05.084
更新日期：2018-09-01 00:00:00
abstract：:Degradation of high molecular weight dextran for obtaining low molecular weight dextran is based on the hydrolysis using chemical and enzymatic methods. Current research study focused on production, purification and characterization of dextranase from a newly isolated strain of Bacillus licheniformis KIBGE-IB25. Dextr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.04.007
更新日期：2015-07-01 00:00:00
abstract：:Laccase is one of a few enzymes that can directly reduce oxygen into water under ambient conditions, while oxidizing a variety of aromatic compounds. Its conjugation with chitosan generates a pH-sensitive functional biomaterial that changes its solubility in response to pH variation. The molecular conjugation between ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.01.003
更新日期：2005-03-01 00:00:00
abstract：:Nanocomposite films containing cellulose nanocrystals (CNC), as the reinforcing phase, and poly(vinyl alcohol) (PVA)/starch (S) blend, as the matrix, were prepared by solution casting technique. For the preparation, 5, 10, 15 wt% CNC suspension were incorporated in blends containing 90 wt% PVA/10 wt% S, 75 wt% PVA/25 ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.05.036
更新日期：2018-09-01 00:00:00
abstract：:Here we report "green" synthesis of gold nanoparticles in solutions of heterocyclic chitosan derivatives (N-(4-imidazolyl)methylchitosan (IMC), N-2-(2-pyridyl)ethylchitosan (2-PEC), and N-2-(4-pyridyl)ethylchitosan (4-PEC)) and show how efficiency of Au(III) binding to polymer influences the Au(III) reduction rate and...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.05.102
更新日期：2016-10-01 00:00:00
abstract：:Administration of nanomaterials based medicinal and drug carrier systems into systemic circulation brings about interaction of blood components e.g. albumin and globulin proteins with these nanosystems. These blood or serum proteins either get loosely attached over these nanocarriers and form soft protein corona or ar...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2021.01.152
更新日期：2021-01-25 00:00:00
abstract：:An L-fucose lectin, ANL from the corneal smears of a mycotic keratitis patient was reported earlier. Interaction of ANL with immortalized Human Corneal Epithelial Cells (HCECs) was studied in order to assign the role of ANL in pathogenesis. ANL showed strong binding to HCECs which could be blocked by L-fucose and muci...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.020
更新日期：2020-12-15 00:00:00
