Abstract:
:The formation of heteroprotein coacervates obtained by the interaction of ovalbumin (Ova) and lysozyme (Lys) was investigated using turbidimetric analysis and the zeta potential at different protein ratios, pH values and concentrations of NaCl. The complexes were formed over a wide pH range with a 1:1 (Ova:Lys) ratio and the highest turbidity was observed at pH 7.5, which optimal biopolymer interactions occurring. The addition of NaCl disfavored formation, even at low concentrations, and suppressed it at 300mM. The complex coacervate formation occurred in the region between the isoelectric points (pI) of the proteins, predominantly by electrostatic interactions but with participation of hydrogen bonds. The structures formed had an average size of ∼2μm, which was well above the isolated proteins, and microscopic analysis revealed that the complexes had a globular structure. The interaction was exothermic and spontaneous with a favorable entropic and unfavorable entropic contribution during interaction.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Santos MB,Costa ARD,Garcia-Rojas EEdoi
10.1016/j.ijbiomac.2017.08.132subject
Has Abstractpub_date
2018-01-01 00:00:00pages
1323-1329eissn
0141-8130issn
1879-0003pii
S0141-8130(17)31987-6journal_volume
106pub_type
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