Abstract:
:The immuno-modulating activities and chemical characteristics of exo-biopolymer (EX-GF) produced by a submerged mycelial culture of Grifola frondosa were studied. The EX-GF was fractionated into EX-GF-Fr.I, II, and III by Sephadex G-100 gel chromatography. Anti-complementary activity of EX-GF-Fr.III was highest (71.1%) among them, and its activation system occurred through both classical and alternative pathways, where the classical pathway found to be major one. Lysosomal enzyme activity and nitric oxide production ability of macrophage were also found to be mediated by EX-GF-Fr.III. The molecular weight of the EX-GF-Fr.I, II, and III was estimated to be about 163, 40, and 2.8 kDa, respectively. Total sugar and protein contents of the three fractions were 80.3, 61.9 and 89.3%, and 17.3, 35.2, and 10.7%, respectively. The sugar and amino acid compositions of the EX-GF-Fr.I, II, and III were also analyzed in detail.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Yang BK,Gu YA,Jeong YT,Jeong H,Song CHdoi
10.1016/j.ijbiomac.2007.02.012subject
Has Abstractpub_date
2007-08-01 00:00:00pages
227-33issue
3eissn
0141-8130issn
1879-0003pii
S0141-8130(07)00075-Xjournal_volume
41pub_type
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