Abstract：
:Co-aggregation plays an important role in processing protein-rich food materials under heterogeneous conditions. The main cause of co-aggregation is an electrostatic attraction between oppositely charged molecules. This study investigated thermal aggregation of β-lactoglobulin (BLG) (pI=5.1) and lysozyme (LYZ) (pI=10.7) as a model for the heterogeneous conditions of a protein solution. BLG and LYZ were more aggregated in the mixture than in the single solutions. Co-aggregation of the BLG-LYZ mixture was not observed below 60°C at which temperature BLG and LYZ retained their native structures. Adding sugars, salts, or amino acids to the BLG-LYZ mixture during the heat treatment revealed the co-aggregation process as follows. (i) All additives tested suppressed both the nucleation and growth of aggregates. (ii) Salts affected nucleation stage to the same degree, except arginine hydrochloride (Arg). (iii) Arg specifically suppressed both nucleation and growth of aggregates. These results indicate that co-aggregation in a protein mixture is more sensitive to the partial unfolding of proteins than that in a single protein solution, due to the presence of electrostatic attraction between different molecules. These results provide new insight into protein aggregation as well as the molecular mechanism of additives under heterogeneous conditions.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Oki S,Iwashita K,Kimura M,Kano H,Shiraki Kdoi
10.1016/j.ijbiomac.2017.10.004subject
Has Abstractpub_date
2018-02-01 00:00:00pages
1428-1437issue
Pt Beissn
0141-8130issn
1879-0003pii
S0141-8130(17)33564-Xjournal_volume
107pub_type
杂志文章abstract：:Cytochrome P450 monooxygenases (CYP450s) and their auxiliary cytochrome P450 reductases (CPRs) are important and commonly involved in the biosynthesis of camptothecin (CPT). To better understand the possible functions of CPRs in planta, we first isolated two CaCPRs genes from Camptotheca acuminata. Sequence analysis r...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.07.141
更新日期：2019-10-01 00:00:00
abstract：:Lectins are proteins capable of specific and reversible binding to mono- and/or oligosaccharides, and within this group, Legume lectins are the most studied. However, most of these studies focus on the Papilionoideae subfamily, with Caesalpinioideae and Mimosoideae lectins being significantly less explored in the lite...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.03.113
更新日期：2020-07-01 00:00:00
abstract：:The solvent accessible surface area (ASA) of the polysaccharides, namely (i) carrageenan (1CAR); (ii) agarose (1AGA); (iii) guaran (GUR); (iv) capsular polysaccharide (1CAP); and (v) hyaluronan (1HUA), have been computed using the solvent accessibility technique of Lee and Richards. The results show that the average v...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(00)00158-6
更新日期：2001-01-10 00:00:00
abstract：:This study was designed to study the chemical composition of Cyclocarya paliurus polysaccharide and inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage. A new elution (0.3% NaCl aqueous solution) of Cyclocarya paliurus polysaccharide (CPP-3) was characterized by different methods such as fo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.055
更新日期：2018-02-01 00:00:00
abstract：:In an attempt to gain insights into the possible relationship between the irradiation-mediated molecular weight reduction and structural modification and the growth-promotion activity, characterization of the polysaccharide before and after irradiation was carried out through Fourier Transform Infrared (FT-IR), Ultrav...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.022
更新日期：2019-03-01 00:00:00
abstract：:Three phase partitioning is a process in which mixing t-butanol with ammonium sulphate with a protein solution leads to the formation of three phases. Generally, the interfacial protein precipitate (formed between upper t-butanol rich and lower aqueous phase) can be easily dissolved back in aqueous buffers. In case of...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.06.008
更新日期：2013-09-01 00:00:00
abstract：:A polyether-urethane based on polytetrahydrofuran containing hydroxypropyl cellulose for biomedical applications was tested for its biocompatibility. Ketoprofen was incorporated (3% and 6%) in the polyurethane matrix as an anti-inflammatory drug. Dynamic vapour sorption method was employed for testing the water sorpti...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.09.026
更新日期：2013-01-01 00:00:00
abstract：:Chitosan, possessing excellent properties, has been drawing broad attention. For the further utilization of chitosan, chemical modification is performed in improving its water solubility and the bioactivities. In the current study, four N,N,N-trimethyl chitosan salts, including N,N,N-trimethyl chitosan citrate (TMCSCi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.06.018
更新日期：2018-10-15 00:00:00
abstract：:Solid keratin-rich waste management is one of essential research area in nowadays. Conventional chemical and high thermal keratin waste decomposition methods are fully explored and not enough effective for future biotechnology perspectives. However, traditional keratin-rich waste decomposition methods could be replace...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.046
更新日期：2018-07-01 00:00:00
abstract：:In the present study, efficient enzymatic methods were developed using a recombinant metagenomic lipase (LipR1) for the synthesis of corresponding esters by the transesterification of five different pharmaceutically important secondary alcohols. The recombinant lipase (specific activity=87m6U/mg) showed maximum conver...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.10.088
更新日期：2017-02-01 00:00:00
abstract：:Selenium-enriched polysaccharides have become a major research focus owing to their high anti-oxidant activities. Selenium-enriched Pleurotus ostreatus polysaccharide (Se-POP) and the native polysaccharide (POP) were obtained by hot water extraction from fresh fruiting bodies of P. ostreatus with or without selenium. ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.12.152
更新日期：2018-05-01 00:00:00
abstract：:Although the anti-diabetic properties of Konjac glucomannan (KGM) have been reported previously; however, the molecular pathways of its anti-diabetic properties are not clear. The present study hypothesized that KGM could mitigate oxidative stress and inflammation. Three doses of KGM (40, 80, 120 mg/kg b.w.) decreased...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.105
更新日期：2020-09-15 00:00:00
abstract：:The emergence of the global pandemic caused by the novel SARS-CoV-2 virus has motivated scientists to find a definitive treatment or a vaccine against it in the shortest possible time. Current efforts towards this goal remain fruitless without a full understanding of the behavior of the virus and its adaptor proteins....
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.09.204
更新日期：2020-12-15 00:00:00
abstract：:In this study, the ε-polylysine (PL)/chitosan (CS) composite films with the PL concentration of 0%, 1%, 3%, 5% and 7% were prepared via casting method. The Fourier transform infrared (FTIR), scanning electron microscope (SEM), mechanical and antibacterial properties of PL/CS composite films, as well as the effect of P...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.07.074
更新日期：2018-10-15 00:00:00
abstract：:Indian cobra (Naja naja) envenomation is frequently reported across Indian subcontinent. Geographical differences in the venom composition of a particular species of snake often leads to inconsistencies in the antivenom neutralization. Consequently, determining the venom proteome from every locale is necessary for the...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.106
更新日期：2020-10-01 00:00:00
abstract：:Fascioliasis, a serious helminth disease of the livestock population, results from infection with the parasite Fasciola. Despite the alarming increase in drug resistance, a safe and fully effective vaccine for fascioliasis is still not available. In the present study, we employed high-throughput immunoinformatics appr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.07.024
更新日期：2019-10-01 00:00:00
abstract：:The neurotoxicity of HIV-1 Tat protein contributes significantly to the pathogenesis of HAND, and hence the attractive therapeutic strategies focusing on Tat-induced neurotoxicity are warranted. Salvia miltiorrhiza have been known to antioxidant property and neuroprotective effects. The Danshen granule is the pharmace...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.146
更新日期：2019-03-01 00:00:00
abstract：:A crosslinked chitosan-glyoxal/TiO2 nanocomposite (CCG/TNC) was synthesized by loading different ratios of TiO2 nanoparticles into polymeric matrix of crosslinked chitosan-glyoxal (CCG) to be a promising biosorbent for methyl orange (MO). Box-Behnken design (BBD) in response surface methodology (RSM) was applied to op...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.02.025
更新日期：2019-05-15 00:00:00
abstract：:Herein, porous sodium alginate/graphite based hybrid hydrogel was fabricated as an effective adsorbent for organic pollutant. Sodium alginate was modified through graft polymerization of acrylic acid and subsequently loaded with graphite powder to enhance its adsorption capability. The synthesized sodium alginate cros...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.01.142
更新日期：2020-04-01 00:00:00
abstract：:Until now, there is a lack of knowledge about the presence of chitin in numerous representatives of corals (Cnidaria). However, investigations concerning the chitin-based skeletal organization in different coral taxa are significant from biochemical, structural, developmental, ecological and evolutionary points of vie...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.04.016
更新日期：2012-07-01 00:00:00
abstract：OBJECTIVE:We aimed to explore the effect of Sappanone A on neurologic damage induced by hypoxia. METHODS:PC-12 cells were pre-treated with Sappanone A and were simulated by hypoxia. miRNA transfection was performed to overexpress or suppress the expression of miR-15a in PC-12 cells. Cell viability, apoptosis, migratio...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.002
更新日期：2019-02-15 00:00:00
abstract：:Coriolus versicolor is an edible medicinal mushroom in China. Two polysaccharides, named as CVPn and CVPa were separated from the dried fruiting bodies of Coriolus versicolor by water extraction and ethanol precipitation. Their chemical structures were well elucidated with overall consideration of monosaccharide compo...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.018
更新日期：2021-01-01 00:00:00
abstract：:The modified collagen with double bonds was electrospun into nanofibers membrane cured with photo-initiated polymerization under UV irradiation. The morphology and chemical structures were characterized by SEM and FTIR. Both the water resistance and mechanical properties were improved after UV-crosslinking. Then 5-Flu...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.029
更新日期：2018-12-01 00:00:00
abstract：:Lectins are proteins that have as one of their main characteristics recognizing and reversibly binding to carbohydrates. In this work, it was possible to purify and characterize a lectin from Parkia panurensis (Leguminosae family; Mimosoideae subfamily) seeds by a combination of the techniques: protein precipitation, ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.102
更新日期：2020-02-15 00:00:00
abstract：:Complex coacervation was achieved by mixing the lysing enzyme from T. harzianum (LYS) with polyallylamine hydrochloride (PAH). We show in this work that the study electrostatic complexes conformation can lead to the formation of dense complexes. We systematically investigated the effects of pH and the mass ratio on th...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.11.266
更新日期：2019-03-01 00:00:00
abstract：:When not incorporated into the casein micelle, isolated κ-casein spontaneously forms amyloid fibrils under physiological conditions, and is a convenient model for researching generic aspects of fibril formation. Ginsenosides have recently attracted much research interest because of the effects on aging diseases, which...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.04.046
更新日期：2015-08-01 00:00:00
abstract：:Sedum dendroideum is popularly known as balsam and used for treatment of inflammatory disorders. Two polysaccharides (RSBAL-H2O and RSBAL-0.5) were isolated from infusion of its dried leaves, using dialysis at 100kDa cut-off membrane and anion exchange chromatography. Methylation and NMR analyses showed that RSBAL-H2O...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.07.122
更新日期：2017-12-01 00:00:00
abstract：:This work studies specific interactions and compatibility between a legumin and a linear carboxylated polysaccharide using gel permeation chromatography, sedimentation analysis, SDS gel electrophoresis, viscometry and phase analysis measurements. It uses the system water/11 S globulin/CMC as a model. Carboxymethylcell...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2005.12.011
更新日期：2006-02-28 00:00:00
abstract：:The phenomenon of polymer adsorption was applied to modify the surface of microcrystalline cellulose (MCC). The active polymer sodium carboxymethyl cellulose (CMCNa), having different molecular weight was used to produce modified MCCs. The average particle size of unmodified MCC was about 14.208 ± 0.064 μm, and it was...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.04.051
更新日期：2019-07-01 00:00:00
abstract：:Nanotechnology-based drug delivery systems have been used to enhance bioavailability and biological activities. Chitosan incorporating curcumin can serve as a biocompatible substitute for metallic nanoparticles in preventing biofilm formation of Streptococcus mutans and plaque on teeth. The interactions between chitos...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.100
更新日期：2018-07-15 00:00:00
