Abstract:
BACKGROUND:Long non-coding RNAs (lncRNAs) can modulate gene expression through different mechanisms, but the fundamental molecular mechanism behind EPIC1 and osteosarcoma (OS) was poorly understood. METHODS:Bone tumor tissues and the matched normal tissues were obtained from 36 OS patients who received tumor resection from 2014 to 2018. The expression of EPIC1 and MEF2D was determined by quantitative real-Time PCR and western blotting. Cell viability and invasion were evaluated by MTT assay and transwell assay. The animal xenograft model was also established. RESULTS:EPIC1 was down-regulated, but MEF2D was up-regulated in OS tissues and OS cell lines. Overexpression of EPIC1 inhibited cell viability and invasion of OS cells. Targeting relationship between EPIC1 and MEF2D was confirmed by RNA pull-down and RNA immunoprecipitation (RIP). The MEF2D protein binding to ubiquitin was significantly increased in OS cells overexpressing EPIC1. The co-transfection with pcDNA-EPIC1 and pcDNA-MEF2D rescued the inhibition of cell viability and invasion caused by the overexpression of EPIC1. Overexpression of EPIC1 suppressed tumor growth in the OS xenograft model. CONCLUSION:Our findings indicated that overexpression of EPIC1 inhibited cell viability and invasion of OS cells by promoting MEF2D ubiquitylation, which provided innovative lncRNA and protein targets for treating OS.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Zhao W,Zhang D,Qin P,Zhang J,Cui X,Gao J,Wang J,Li Jdoi
10.1016/j.ijbiomac.2019.01.156subject
Has Abstractpub_date
2019-05-01 00:00:00pages
566-573eissn
0141-8130issn
1879-0003pii
S0141-8130(18)36883-1journal_volume
128pub_type
杂志文章abstract::Crosslinked chitosan beads were grafted with N-vinyl-2-pyrrolidone (NVP) using ammonium persulfate (APS) as free radical initiator. Important variables on graft copolymerization such as temperature, reaction time, concentration of initiator and concentration of monomer were optimized. The results revealed optimum cond...
journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2019.04.016
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章
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更新日期:2019-12-01 00:00:00
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章,评审
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2018.07.026
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2006.08.009
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
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journal_title:International journal of biological macromolecules
pub_type: 杂志文章
doi:10.1016/j.ijbiomac.2017.09.020
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abstract::Polysaccharides are essential compounds that contribute to the biological activities of mushrooms. Two new galactomannans (Galactomannan I and II) were isolated from R. luteolus and G. adspersum. Their structures were characterized using FT-IR, 1D, and 2D-NMR techniques. Both isolated galactomannans I and II mainly in...
journal_title:International journal of biological macromolecules
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abstract::In the present study a thiol proteinase inhibitor was isolated from buffalo kidney making use of ammonium sulphate precipitation and gel filtration chromatography on Sephacryl S-100HR column. Purified inhibitor is homogeneous as it displayed a single band in gel electrophoresis both under reducing and non-reducing env...
journal_title:International journal of biological macromolecules
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