Abstract:
:The modification of gum Arabic with ferulic acid oxidation products was performed in aqueous medium, at 30 °C and pH 7.5, in the presence of Myceliophthora thermophila laccase as biocatalyst. First, this study aimed to investigate the structures of the oxidation products of ferulic acid that could possibly be covalently grafted onto gum Arabic. HPLC analyses revealed that this reaction produced several oxidation products, whose structures were investigated using LC-MS/MS analyses (liquid chromatography-mass spectrometry with mass fragmentation analyses) and NMR experiments. The chemical structure of one intermediate reaction product was fully elucidated as the 2-(4-hydroxy-3-methoxyphenyl)-4-[(4-hydroxy-3-methoxyphenyl) methylidene] cyclobutane-1, 3-dione, called by the authors cyclobutadiferulone. Secondly, this study aimed to locate the grafting of the oxidation products onto gum Arabic by performing several NMR experiments. This study did not determine how much and specifically which oxidation products were grafted but some of them were undeniably present onto modified gum Arabic, close to the glucuronic acid C5 carbon or close to the galactose C6 carbon.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Vuillemin ME,Muniglia L,Linder M,Bouguet-Bonnet S,Poinsignon S,Dos Santos Morais R,Simard B,Paris C,Michaux F,Jasniewski Jdoi
10.1016/j.ijbiomac.2020.12.113subject
Has Abstractpub_date
2021-02-01 00:00:00pages
480-491eissn
0141-8130issn
1879-0003pii
S0141-8130(20)35284-3journal_volume
169pub_type
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