Abstract:
:Polysaccharides are an abundant resource in coffee beans and have proved to show numerous bioactivities. Despite their abundance, their activities are not always satisfactory mostly due to their structure and large molecular size. Molecular modifications of native polysaccharides can overcome this problem. In this study, we used a novel and green method to modify native coffee polysaccharides using subcritical water (SCW) treatment. The SCW treatment was used at the temperature of 180°C-220°C and pressure of 30-60bar. The molecular and structural modification of the polysaccharides was confirmed using several techniques such as FT-IR, UV spectroscopy, XRD, and TGA. The antioxidant activity of the modified polysaccharides was evaluated using several chemical and Saccharomyces cerevisiae-based high throughput assays. The modified polysaccharides showed high antioxidant activities in all tested assays. Moreover, the polysaccharides showed high DNA protection activities. Therefore, SCW could be employed as a green solvent for molecular modification of polysaccharides.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Getachew AT,Chun BSdoi
10.1016/j.ijbiomac.2017.03.034subject
Has Abstractpub_date
2017-06-01 00:00:00pages
555-562eissn
0141-8130issn
1879-0003pii
S0141-8130(17)30107-1journal_volume
99pub_type
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