Abstract：
:Arginase is one of the key enzymes responsible for maintaining the essential levels of nitrogen among plants, but biochemical and functional characterization of arginase among plants is limited. While screening for stable plant arginase, we found cilantro possessing an abundant and stable arginase. We purified arginase to apparent homogeneity (3300-fold purification) with a specific activity of 81,728 nmoles of urea formed/mg of protein/min and its eight-tryptic fragments had amino acid sequences identical to Arabidopsis thaliana arginase. Cilantro arginase exhibited absolute requirement for Mn2+ (0.5 mM-1 mM). Unlike other known plant arginases, cilantro arginase did not hydrolyse d-arginine and other arginine analogues. While for sulfhydryl reagents the enzyme was sensitive, l-NOHA, an arginase inhibitor showed only moderate inhibition - a property distinct from tomato arginase. We also found arginine derived amino acids and polyamines can regulate cilantro arginase in vitro. In addition, we also noticed an increase in cilantro arginase activity to both biotic and abiotic stress. We conclude that, cilantro may be used as a model plant to study plant arginases and to delineate arginase role, beyond its classical role in nitrogen recycling and polyamine biosynthesis.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Siddappa S,Basrur V,Ravishankar Rai V,Marathe GKdoi
10.1016/j.ijbiomac.2018.06.096subject
Has Abstractpub_date
2018-10-15 00:00:00pages
844-856issue
Pt Aeissn
0141-8130issn
1879-0003pii
S0141-8130(18)30481-1journal_volume
118pub_type
杂志文章abstract：:The aim of this study was to optimize the mechanical and texture properties of edible film improving its antibacterial property after adding rosemary essential oil (REO) using a Doehlert matrix. Films with the highest mechanical properties were acquired using a polymer composition of 65.2% glycerol, 24.3% gelatin, 10....
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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abstract：:Chitosan (CHI)-paramagnetic ion interactions in aqueous solution as affected by pH were studied using time-domain NMR (TD-NMR) relaxometry. Longitudinal (T1) and transverse (T2) relaxation times of CHI solutions with Fe3+, Cu2+, and Mn2+ were measured using a single-shot pulse sequence named CP-CWFPx-x. The results in...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.083
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abstract：:In this study, ecofriendly starch/TiO2 bio-nanocomposites were produced using with different nano-TiO2 (TiO2) content (1, 3, and 5 (wt%)). Physical, mechanical, thermal, water-vapor permeability (WVP) properties and UV transmittance were investigated. Our results showed that the increasing TiO2 content increased the h...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.11.065
更新日期：2017-02-01 00:00:00
abstract：:Poly (lactic acid)-based functional films incorporated with curcumin have been prepared using a solution casting method. The PLA/curcumin composite film was flexible and highly transparent with bright yellow color. Curcumin was uniformly dispersed in the PLA matrix and showed excellent compatibility with PLA, as evide...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-11-01 00:00:00
abstract：:A chitosan-silver nanoparticle solution (CSNS) was applied as a coating material to Hanji (Korean traditional paper), and the properties of the coated paper were investigated as a function of the dilution ratio. The required CSNS was first prepared from AgNO3 (30mmol) by utilizing chitosan as a reducing and stabilizin...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.09.067
更新日期：2016-12-01 00:00:00
abstract：:It is of great clinical significance to design wound dressing materials with combined excellent wound healing properties and superior capability to suppress hypertrophic scar formation. This study aimed to examine if and how the cationicity of chitosan would affect the hypertrophic scar-related outcomes, through prepa...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.161
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abstract：:An emerging paradigm in wound healing techniques is that a tissue-engineered skin substitute offers an alternative approach to create functional skin tissue. Here we developed a fish collagen/alginate (FCA) sponge scaffold that was functionalized by different molecular weights of chitooligosaccharides (COSs) with the ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.08.038
更新日期：2015-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.11.075
更新日期：2016-03-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2019-05-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.225
更新日期：2021-01-07 00:00:00
abstract：:Chitin and chitosan are amino polysaccharides having massive structural propensities to produce bioactive materials with innovative properties, functions and diverse applications particularly in biomedical field. The specific physico-chemical, mechanical, biological and degradation properties offer efficient way to bl...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2016.02.004
更新日期：2016-05-01 00:00:00
abstract：:A gene, encoding a new alginate lyase AlgNJU-03, was cloned from marine bacteria Vibrio sp. NJU-03. The recombinant alginate lyase was characterized followed by being purified by NTA-Ni Sepharose. It exhibited the highest activity (6468.99U/mg) at pH 7.0 and 30°C. Interestingly, AlgNJU-03 possessed broader substrate s...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.164
更新日期：2018-03-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.126
更新日期：2020-12-15 00:00:00
abstract：:Given the great demand for biopolymer and protein-based products from renewable resources, synthesis of a keratin-based hydrogel is presented herein. In this work, a novel hydrogel of poly(γ-glutamic acid) (γ-PGA) and keratin was synthesized through facile EDC·HCl/HOBt chemistry. Since keratin main chain is rich in am...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.10.020
更新日期：2020-01-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.09.009
更新日期：2013-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.12.084
更新日期：2017-01-01 00:00:00
abstract：:As an important direct solvent for cellulose, N-methyl morpholine N-oxide (NMMO) is environmentally friendly, and potentially very economical. Silk fibroin (SF) (Bombyx mori) can also be dissolved directly in NMMO.H2O. However, it is unexpectedly difficult to obtain a silk fibroin solution with a concentration higher ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2004.11.008
更新日期：2005-04-01 00:00:00
abstract：:"Go Green" campaign is gaining light for various industrial applications where water consumption needs to be reduced. To resolve this, industries have adopted usage of green, organic solvents, as an alternative to water. For leather making, tanning industry consumes gallons of water. Therefore, for adopting green solv...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.05.021
更新日期：2014-08-01 00:00:00
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journal_title：International journal of biological macromolecules
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(99)00015-x
更新日期：1999-06-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-09-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2018-05-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2013-04-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2014-04-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2016-11-01 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2021-02-15 00:00:00
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journal_title：International journal of biological macromolecules
pub_type： 杂志文章
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更新日期：2020-12-01 00:00:00
abstract：:Myeloid-derived suppressor cells (MDSCs) accumulate in tumor-bearing hosts and play a major role in tumor-induced immunosuppression. The potent modulatory effects of polysaccharides on the innate and adaptive immune system stimulate antitumor responses. In this study, a polysaccharide with an apparent molecular weight...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.04.153
更新日期：2018-08-01 00:00:00
abstract：:Poly(ε-caprolactone) (PCL) is one of the most commonly used polymers in the production of tissue engineered scaffolds for hard tissue treatments. Incorporation of cells into these scaffolds significantly enhances the healing rate of the tissue. In this study, PCL scaffolds were prepared by wet spinning technique and m...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.12.036
更新日期：2019-03-15 00:00:00
