Abstract：
:Under favorable growth conditions, Staphylococcus aureus infects and causes illnesses in humans and animals. Staphylococcal enterotoxins are considered the most important factor for food poisoning, with these toxins usually found in milk and its byproducts. This study investigated the presence of a classical enterotoxin of S. aureus (enterotoxin A) in raw cow milk samples obtained from Region 3 of Tehran. Sixty cow milk samples were collected from traditional dairy sales centers in the study site. Electrophoresis, high-performance liquid chromatography, sandwich ELISA, and direct ELISA were evaluated as methods for detecting toxins in the milk. A direct ELISA kit was designed, with the best factors, the most suitable densities, and the most optimal temperatures and temporal incubation conditions incorporated into the design. Among the evaluated methods, direct ELISA exhibited the highest accuracy in toxin detection. The designed kit achieved a correctness coefficient of 0.98 and detected enterotoxin A in 23% of the samples. It also exhibited a relative sensitivity and other features that are comparable to those of other bacterial detection techniques. Finally, the kit achieved good matching and repetition results, and no cross-reactions occurred between enterotoxins during the procedure.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Nouri A,Ahari H,Shahbazzadeh Ddoi
10.1016/j.ijbiomac.2017.10.052subject
Has Abstractpub_date
2018-02-01 00:00:00pages
1732-1737issue
Pt Beissn
0141-8130issn
1879-0003pii
S0141-8130(17)32544-8journal_volume
107pub_type
杂志文章abstract：:Agrobacterium tumefaciens uronate dehydrogenase (AtuUdh) belongs to the short-chain dehydrogenase superfamily, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. It is apparently required for the production of D-glucaric acid. AtuUdh-catalyzed reaction is reversible with dual substra...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.194
更新日期：2019-11-01 00:00:00
abstract：:To fight oxidative damage due to reactive oxygen species (ROS), cells are equipped of different enzymes, among which Peroxiredoxins (Prxs) (EC 1.11.1.15) play a key role. Prxs are thiol-based enzymes containing one (1-Cys Prx) or two (2-Cys Prx) catalytic cysteine residues. In 2-Cys Prxs the cysteine residues form a d...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.052
更新日期：2020-06-15 00:00:00
abstract：:In the present study, the formation of one molten globule-like unfolding intermediate of bovine serum albumin (BSA) at pH 11.2 has been established with the help of circular dichroism (CD) spectra, fluorescence spectroscopy and 'phase diagram' approach. Additionally, we have shown the conformational changes occurring ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.04.021
更新日期：2012-07-01 00:00:00
abstract：:A new green hydrogel has been synthesized by crosslinking lignosulfonate (L), sodium alginate (SA) and konjaku flour (KJ). We have optimized the ratio of the three synthesized polymers using an orthogonal design of experiments and characterized this hydrogel using SEM, BET and FTIR spectra. We have added the hydrogel ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.12.082
更新日期：2020-02-01 00:00:00
abstract：:Coriolus versicolor has been known to be an immune stimulator effects. For further understanding of the phagocytic activity and the intracellular mechanisms of β-glucan from C. versicolor (CVG), we examined the phagocytic activity, phosphorylation of Akt and CK2, nucleus translocation of p65 and Ikaros activity in β-g...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.03.017
更新日期：2013-06-01 00:00:00
abstract：:A polysaccharide was isolated from the sheath of a sheathed bacterium, Sphaerotilus natans. The sheath polysaccharide (SPS) was composed of D-glucose and D-(N-acetyl)galactosamine in molar ratios of 1:4. Methylation linkage analysis revealed the presence of the residues of 4-linked glucose, 4-linked (N-acetyl)galactos...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2003.08.008
更新日期：2003-12-01 00:00:00
abstract：:The purpose of this study was to gain insights into the hydrolysis characteristics of xylanase in producing xylo-oligosaccharides and to improve these by protein engineering. In this study, a hybrid enzyme (T-XynFM) was created from Talaromyces thermophilus F1208 xylanase (T-Xyn) by replacement of the N-terminal exten...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.12.050
更新日期：2017-03-01 00:00:00
abstract：:Codonopsis pilosula polysaccharide (CPPS) and selenizing CPPS (sCPPS) were prepared and identified by a combination of chemical and instrumental analysis. Their immune modulation activities were compared by lymphocyte proliferation and flowcytometry tests in vitro or serum antibody responses and cytokines with immuniz...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.149
更新日期：2020-10-01 00:00:00
abstract：:A simple and valid ultraviolet (UV) spectrophotometric method for the determination of poly(γ-glutamic acid) is developed. The method is based on the UV absorption spectrum of γ-PGA in aqueous solution, which exhibits a maximum absorption wavelength at 216 nm. The results obtained were comparable to those obtained wit...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2012.04.005
更新日期：2012-07-01 00:00:00
abstract：:Possible interaction between immunosuppressive drug, azathioprine, and calf thymus DNA was explored by cyclic voltammetry, spectrophotometry, competitive spectrofluorimetry, circular dichroism spectroscopy (CD), and viscosity measurements. Cyclic voltammetry showed negative shift in the reduction peak of azathioprine ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.08.025
更新日期：2015-11-01 00:00:00
abstract：:The most critical issues faced by the world nowadays is to provide the sustainability of consumption for energy and natural resources. Lignin is said to be one of the alternative new discoveries best-suited lignocellulosic biomass due to its low cost, sufficient availability and environmentally safe. The valuable prop...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.06.168
更新日期：2020-11-01 00:00:00
abstract：:In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.01.033
更新日期：2014-04-01 00:00:00
abstract：:Monoclonal antibodies (mAbs) and their derivatives have achieved remarkable success as medicine, targeting both diagnostic and therapeutic applications associated with communicable and non-communicable diseases. In the last 3 to 4 decades, tremendous success has been manifested in the field of cancer therapy, autoimmu...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2019.06.006
更新日期：2019-08-15 00:00:00
abstract：:One popular and relevant proposed function for cyanobacterial hemoglobin (Synechocystis Hb) is anaerobic nitrite reductase in vivo. During such reduction reactions, the hexacoordinated heme iron atom of SynHb is oxidized from the ferrous (Fe+2) to ferric (Fe+3) state and prevent damage by limiting the concentration of...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.06.214
更新日期：2020-11-01 00:00:00
abstract：:The interaction of novel oligo(L-lysine)-shelled dendrimers (G3-PLL) with DNA was studied by means of circular dichroism spectroscopy, dynamic light scattering, and melting behavior of double-stranded DNA. G3-PLLs having various oligo(L-lysine) (PLL) segment (n = 5-40) were successfully synthesized by graft-polymeriza...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.01.017
更新日期：2006-03-30 00:00:00
abstract：:The ability of Delftia acidovorans to incorporate a broad range of 3-hydroxyvalerate (3HV) monomers into polyhydroxyalkanoate (PHA) copolymers was evaluated in this study. Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] containing 0-90 mol% of 3HV was obtained when a mixture of sodium 3-hydroxybutyrate an...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2006.11.003
更新日期：2007-04-10 00:00:00
abstract：:Lyophilized wafers comprising sodium alginate (SA) and gelatin (GE) (0/100, 75/25, 50/50, 25/75, 0/100 SA/GE, respectively) with silver sulfadiazine (SSD, 0.1% w/w) have been developed for potential application on infected chronic wounds. Polymer-drug interactions and physical form were characterized by Fourier transf...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.04.048
更新日期：2015-08-01 00:00:00
abstract：:Glycol chitosan/fucoidan nanogels loaded with anti-inflammatory peptide KAFAK (GC/Fu@KAFAK NGs) were fabricated based on the electrostatic interaction and genipin cross-linking methods. The prepared NGs had an average size of 286.3 ± 5.0 nm and positive surface charge of 14.0 ± 0.2 mV. The anti-inflammatory and chondr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.12.158
更新日期：2021-02-15 00:00:00
abstract：:The aim of this work was to prepare pectin-poly (vinyl pyrrolidone) [PVP] based curcumin particulates to enhance the anticancer potential of curcumin, solubility and allow its localized controlled release. Pectin-PVP based curcumin particulates (PECTIN-PVP CUR) were prepared by spray drying technique in different rati...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.05.170
更新日期：2017-11-01 00:00:00
abstract：:Thiothrix fructosivorans forms a microtube (sheath) that encloses a line of cells. This sheath is an assemblage of [→4)-GlcN-(1→4)-Glc-(1→]n with side chains of Rha4N-(1→3)-Fuc(1→ at position 3 of Glc. The sheath-forming polysaccharide (SFP) may have some substitutions but this is not yet confirmed. To investigate the...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.10.028
更新日期：2016-01-01 00:00:00
abstract：:We report cloning and expressing of recombinant human VEGF-A165, fused at the N-terminal with Hydrophobin II (HFBII) from Trichoderma reseei, in yeast Pichia pastoris. We validated the construct using SDS-PAGE and ELISA against VEGF-A165 and efficiently performed protein purification and enrichment based on HFBII coun...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.08.080
更新日期：2019-10-15 00:00:00
abstract：:Two types of the biological macromolecules poly(R-3-hydroxyalkanoates) have been identified: the high-molecular-weight microbial storage material (sPHA) and a short-chain variety, consisting of butyrate and valerate residues, complexed with other biomacromolecules such as calcium polyphosphate or proteins (cPHB/PHV). ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/s0141-8130(99)00037-9
更新日期：1999-06-01 00:00:00
abstract：:The main objective of this work was to study the possibility of using polysaccharides and lignin as a coating material for water-soluble triple superphosphate (TSP) granular fertilizers. In this study, composites based on three polysaccharides (sodium alginate (alginic acid sodium salt): AL, kappa-carrageenan: CR and ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.12.005
更新日期：2020-01-15 00:00:00
abstract：:The modified collagen with double bonds was electrospun into nanofibers membrane cured with photo-initiated polymerization under UV irradiation. The morphology and chemical structures were characterized by SEM and FTIR. Both the water resistance and mechanical properties were improved after UV-crosslinking. Then 5-Flu...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.09.029
更新日期：2018-12-01 00:00:00
abstract：:Staphylopine is a newly identified broad-spectrum metallophore for metal acquisition, and it plays important roles in the survival and virulence of Staphylococcus aureus and other pathogens in the metal-scarce environment in hosts. CntK catalyzes the first step of staphylopine synthesis by converting L-histidine to D-...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.169
更新日期：2019-08-15 00:00:00
abstract：:An L-fucose lectin, ANL from the corneal smears of a mycotic keratitis patient was reported earlier. Interaction of ANL with immortalized Human Corneal Epithelial Cells (HCECs) was studied in order to assign the role of ANL in pathogenesis. ANL showed strong binding to HCECs which could be blocked by L-fucose and muci...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.020
更新日期：2020-12-15 00:00:00
abstract：:Beta-lactoglobulin has been found to be a member of a super family of protein that bind specific ligands and which share common features in their amino acid sequences. Here we show that these features are grouped spatially on the surface of the proteins and suggest that they may be concerned with binding to cell-surfa...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/0141-8130(89)90041-x
更新日期：1989-02-01 00:00:00
abstract：:The carboxymethylated (1 → 6)-α-dextran (CM-dex) was synthesized by introducing carboxymethyl groups at different degrees of substitution (DS). The resulting dex1-1, dex2-1, dex3-1, and dex4-1 products had degrees of substitution of 0.57, 0.78, 1.13, and 1.25, respectively. The dex3-1 showed the highest glass transiti...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.11.114
更新日期：2021-01-01 00:00:00
abstract：:In the present study, a potential Guar gum/activated carbon nanocomposite as an adsorbent was synthesized and utilized for the sequestration of toxic congo red dye from synthetic wastewater. The nanocomposite was characterized using different techniques such as SEM, EDX, TEM, FTIR and XRD. Various physico-chemical par...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.05.025
更新日期：2020-05-07 00:00:00
abstract：:In the present work, N,N,N-trimethyl chitosan (TMC) fibers were synthesized successfully and the resulting quaternized materials were characterized by FTIR. The designed TMC fibers with different degree of quaternization achieved high water absorption capability. In antibacterial activity study, TMC fibers showed high...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.02.036
更新日期：2016-08-01 00:00:00
