Abstract:
:The cDNA of the β-1,4-endoxylanase of Aspergillus niger US368 was cloned and expressed in Pichia pastoris under the constitutive GAP promoter. The maximum activity obtained was 41 U mL(-1), which was about 3-fold higher than that obtained with the native species. The purified enzyme showed a specific activity of 910 U mg(-1) and a molecular mass of 24 kDa. It had an optimal activity at pH 4 and 50 °C, stable in a wide range of pH and in the presence of some detergents and organic solvents. r-XAn11-His6 (recombinant xylanase) was used as an additive in breadmaking. A decrease in water absorption, an increase in dough rising and improvements in volume and specific volume of the bread were recorded. The r-XAn11-His6 was also used in in vitro digestion of barley and wheat bran leading to a decrease of the viscosities and an increase of the reducing sugars and total sugars contents.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Elgharbi F,Hmida-Sayari A,Zaafouri Y,Bejar Sdoi
10.1016/j.ijbiomac.2015.04.053subject
Has Abstractpub_date
2015-08-01 00:00:00pages
103-9eissn
0141-8130issn
1879-0003pii
S0141-8130(15)00291-3journal_volume
79pub_type
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