Abstract:
:Aureobasidium melanogenum TN3-1 isolated from a natural honey was a highly genome-duplicated yeast-like fungal strain and a very high pullulan producer. In this study, simultaneous removal of both duplicated AMY1 genes encoding α-amylase and duplicated PKS1 genes responsible for melanin biosynthesis in A. melanogenum TN3-1 rendered a mutant AMY-PKS-11 to transform 140.0 g/L of glucose to produce 103.50 g/L of pigment-free pullulan with molecular weight (Mw) of 3.2 × 105 g/mol. α-Amylase activity produced by the mutant AMY-PKS-11 and expression of the AMY1 genes and PKS genes in it was reduced, but expression of various genes responsible for pullulan biosynthesis in the mutant AMY-PKS-11 was up-regulated. The produced pullulan was used to make the capsule shells successfully and the prepared pullulan capsule shells had various advantages such as high strength, good oxygen barrier properties, raw materials availability, tightness, lightness and high water resistance and may be suitable for all the consumers. Therefore, the prepared capsule shells had highly potential applications in food and pharmaceutical industries.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Chen L,Chi Z,Liu GL,Xue SJ,Wang ZP,Hu Z,Chi ZMdoi
10.1016/j.ijbiomac.2019.08.264subject
Has Abstractpub_date
2019-12-01 00:00:00pages
268-277eissn
0141-8130issn
1879-0003pii
S0141-8130(19)35666-1journal_volume
141pub_type
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