Expression and biochemical characterization of recombinant α-l-rhamnosidase r-Rha1 from Aspergillus niger JMU-TS528.


:A putative cDNA of α-l-rhamnosidase was PCR-cloned from Aspergillus niger JMU-TS528 and further extracellular over-expressed in Pichia pastoris GS115. The activity of the recombinant α-l-rhamnosidase r-Rha1 was 711.9U/mL, eightfold higher than the native α-l-rhamnosidase from A. niger JMU-TS528. r-Rha1 is a N-glycosylated protein of 90kDa and possesses broad substrate specificities by hydrolyzing α-1,2, α-1,3 α-1,4, and α-1,6 linkages to β-d-glucosides. This is the first report presenting that α-l-rhamnosidase showed activity on four kinds of glucosidic linkages. Compared with other previously characterized α-l-rhamnosidases, r-Rha1 showed a good thermostability and wide range of pH-stability with the optimum pH of 5.0 and temperature of 60°C. r-Rha1 activity was not greatly affected by representative metal ions and other detected effectors and showed excellent tolerance abilities against glucose and ethanol. These beneficial characteristics of r-Rha1 suggest that r-Rha1 should be considered a potential new biocatalyst for food and drug industrial applications.


Int J Biol Macromol


Li L,Yu Y,Zhang X,Jiang Z,Zhu Y,Xiao A,Ni H,Chen F




Has Abstract


2016-04-01 00:00:00












  • Microbial-based synthesis of highly elastomeric biodegradable poly(3-hydroxybutyrate-co-4-hydroxybutyrate) thermoplastic.

    abstract::This study reports the production of P(3HB-co-4HB) [Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)] in possession of high molecular weight and elastomeric properties by Cupriavidus sp. USMAA1020 in single-stage mixed-substrate cultivation system. 1,4-butanediol and 1,6-hexanediol are found to be efficient substrate mixt...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Huong KH,Teh CH,Amirul AA

    更新日期:2017-08-01 00:00:00

  • Mathematical modeling of gallic acid release from chitosan films with grape seed extract and carvacrol.

    abstract::Controlled release of antimicrobial and antioxidant compounds from packaging films is of utmost importance for extending the shelf-life of perishable foods. This study focused on the mathematical modeling of gallic acid release into an aqueous medium from three chitosan films, formulated with grape seed extract (GSE) ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Rubilar JF,Cruz RMS,Zuñiga RN,Khmelinskii I,Vieira MC

    更新日期:2017-11-01 00:00:00

  • Temperature effects on alpha-crystallin structure probed by 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone, an environmentally sensitive two-wavelength fluorescent dye covalently attached to the single Cys residue.

    abstract::The single Cys residue in the C-terminal domain of bovine eye lens alpha-crystallin was covalently labelled with 6-bromomethyl-2-(2-furanyl)-3-hydroxychromone. This novel SH-reactive two-band ratiometric fluorescent dye is characterized by excited state intramolecular proton transfer reaction yielding two highly emiss...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Avilov SV,Bode C,Tolgyesi FG,Klymchenko AS,Fidy J,Demchenko AP

    更新日期:2005-09-28 00:00:00

  • Furan-modified natural rubber: A substrate for its reversible crosslinking and for clicking it onto nanocellulose.

    abstract::The conventional vulcanization process applied to elastomers is irreversible and hinders therefore their useful recycling. We demonstrate here that natural rubber can be reversibly crosslinked via the Diels-Alder coupling of furan and maleimide moieties. The furan-modified natural rubber used in this strategy was also...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Trovatti E,Cunha AG,Carvalho AJ,Gandini A

    更新日期:2017-02-01 00:00:00

  • Alginate bionanocomposite films containing sepiolite modified with polyphenols from myrtle berries extract.

    abstract::Alginate nanocomposite films incorporating sepiolite (Sep) modified with myrtle berries extract (MBE) rich in polyphenols were prepared by solution casting method. The effects of different extract concentrations on the film properties were determined by measuring physicochemical, mechanical and antioxidant properties ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Cheikh D,Martín-Sampedro R,Majdoub H,Darder M

    更新日期:2020-12-15 00:00:00

  • Hydroxypropylation of cross-linked sesbania gum, characterization and properties.

    abstract::Sesbania gum (SG) was successfully modified by combining cross-linking with hydroxypropylation in solvent of ethanol. The hydroxypropylation technology parameters were optimized by the orthogonal test. The results indicated that both cross-linking and hydroxypropylation could improve the freeze-thaw stability, acid an...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Tang H,Liu Y,Li Y,Li Q,Liu X

    更新日期:2020-06-01 00:00:00

  • Inhibitory kinetics and bioactivities of Nuciferine and Methyl Ganoderate on Mucor miehei lipase and 3T3-L1 preadipocytes.

    abstract::In this study, inhibitory kinetics of Nuciferine and Methyl Ganoderate extrated from Lotus Leaves and Ganoderma lucidum on Mucor miehei Lipase were studied first. The molecular structure of Nuciferine and Methyl Ganoderate were determined. The inhibitory effects of two extracts on lipase were reversible, with the IC50...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Liu XT,Liu TT,Xu HL,Chen QX,Wang Q

    更新日期:2020-11-15 00:00:00

  • Exploration of the polar microenvironment around the reactive cysteine in rabbit muscle creatine kinase.

    abstract::The polar microenvironment around the reactive Cys283 of rabbit muscle creatine kinase was explored using kinetic analysis of substrates reaction in the presence of modifiers. In the present study, three specific sulphydryl reagents, 5,5'-dithiobis(2-nitrobenzoic acid), 6,6'-dithiodinicotinic acid and 2,2'-dithiodipyr...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: He HW,Li J,Zhao TJ,Ma Y,Shi F,Zhou HM

    更新日期:2007-10-01 00:00:00

  • Demonstration of the adhesive properties of the medium-chain-length polyhydroxyalkanoate produced by Pseudomonas chlororaphis subsp. aurantiaca from glycerol.

    abstract::Pseudomonas chlororaphis subsp. aurantiaca (DSM 19603) was grown on crude glycerol from biodiesel production to produce a medium-chain-length polyhydroxyalkanoate (mcl-PHA), composed of 3-hydroxydodecanoate (43 ± 1.8 mol%), 3-hydroxydecanoate (29 ± 3.1 mol%), 3-hydroxytetradecanoate (12 ± 0.4 mol%), 3-hydroxyoctanoate...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Pereira JR,Araújo D,Marques AC,Neves LA,Grandfils C,Sevrin C,Alves VD,Fortunato E,Reis MAM,Freitas F

    更新日期:2019-02-01 00:00:00

  • Nano photo scouring and nano photo bleaching of raw cellulosic fabric using nano TiO2.

    abstract::Photo catalytic action of nano TiO(2) for decomposing of some organic compounds is a well known phenomenon. This can be extended to the application on nano TiO(2) on the desized cotton fabric to decompose the hydrophobic impurities and coloring matters of the fabric. This can be nominating as a replacement for the con...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Montazer M,Morshedi S

    更新日期:2012-05-01 00:00:00

  • Physicochemical and in vitro digestion of millet starch: Effect of moisture content in microwave.

    abstract::In this study, the physicochemical and in vitro digestion of millet starch with different moisture content after microwave treatment were investigated. The structure and property changes of native (NA) and microwave treated (MT) millet starches were characterized using Brabender viscometer, Fourier transforms infrared...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Li Y,Hu A,Wang X,Zheng J

    更新日期:2019-08-01 00:00:00

  • Exploring structural aspects of the human Golgi matrix protein GRASP55 in solution.

    abstract::In mammals, the Golgi apparatus is the central hub for intracellular trafficking, sorting and post-translational modifications of proteins and lipids. Golgi reassembly and stacking proteins (GRASPs) are somehow involved in Golgi stacking, which is relevant for its proper function, and also in unconventional protein se...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Reddy ST,Mendes LFS,Fontana NA,Costa-Filho AJ

    更新日期:2019-08-15 00:00:00

  • Surface decoration of neohesperidin-loaded nanoliposome using chitosan and pectin for improving stability and controlled release.

    abstract::The aim of this study was to improve the physicochemical stability of neohesperidin (NH) using nanoliposomal encapsulation in association with surface decoration strategy employing chitosan (CH) and pectin (P). Different nanoliposomal systems, i.e. NH-loaded nanoliposome (NH-NL), CH-coated NH-NL (CH-NH-NL), and P-coat...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Karim N,Shishir MRI,Chen W

    更新日期:2020-12-01 00:00:00

  • Substrate-imprinted docking of Agrobacterium tumefaciens uronate dehydrogenase for increased substrate selectivity.

    abstract::Agrobacterium tumefaciens uronate dehydrogenase (AtuUdh) belongs to the short-chain dehydrogenase superfamily, specifically those acting on the CH-OH group of donor with NAD+ or NADP+ as acceptor. It is apparently required for the production of D-glucaric acid. AtuUdh-catalyzed reaction is reversible with dual substra...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Murugan A,Prathiviraj R,Mothay D,Chellapandi P

    更新日期:2019-11-01 00:00:00

  • Molecular recognition between pancreatic lipase and natural and synthetic inhibitors.

    abstract::Pancreatic lipase (PL) is a primary lipase critical for triacylglyceride digestion in humans and is considered as a promising target for the treatment of obesity. Although the current synthetic drugs available for treating obesity have been demonstrated to be effective in inhibiting PL, their prolonged usage results i...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Bello M,Basilio-Antonio L,Fragoso-Vázquez J,Avalos-Soriano A,Correa-Basurto J

    更新日期:2017-05-01 00:00:00

  • Improved thermal-stability and mechanical properties of type I collagen by crosslinking with casein, keratin and soy protein isolate using transglutaminase.

    abstract::The inferior thermal- stability of collagen hinders its extensive application in food industry, including edible packaging. To improve the thermal- stability and mechanical properties of collagen, we attempted to crosslink collagen with some proteins possessing excellent thermal stability (i. e., casein, keratin and s...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Wu X,Liu Y,Liu A,Wang W

    更新日期:2017-05-01 00:00:00

  • Improving special hydrolysis characterization into Talaromyces thermophilus F1208 xylanase by engineering of N-terminal extension and site-directed mutagenesis in C-terminal.

    abstract::The purpose of this study was to gain insights into the hydrolysis characteristics of xylanase in producing xylo-oligosaccharides and to improve these by protein engineering. In this study, a hybrid enzyme (T-XynFM) was created from Talaromyces thermophilus F1208 xylanase (T-Xyn) by replacement of the N-terminal exten...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Li Q,Sun B,Xiong K,Teng C,Xu Y,Li L,Li X

    更新日期:2017-03-01 00:00:00

  • Some physicochemical and rheological properties of starch isolated from avocado seeds.

    abstract::Seeds from avocado (Persea americana Miller) fruit are a waste byproduct of fruit processing. Starch from avocado seed is a potential alternative starch source. Two different extraction solvents were used to isolate starch from avocado seeds, functional and rheological characteristics measured for these starches, and ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Chel-Guerrero L,Barbosa-Martín E,Martínez-Antonio A,González-Mondragón E,Betancur-Ancona D

    更新日期:2016-05-01 00:00:00

  • Enzymatic collapse of artificial polymer composite material containing double-stranded DNA.

    abstract::Large amounts of DNA-enriched biomaterials, such as salmon milts and shellfish gonads, are discarded as industrial waste around the world. Therefore, the utilizations of DNA with the specific function are important for the biomaterial science and the curce technology. We could convert the discarded DNA to an enzymatic...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Yamada M,Amoo M

    更新日期:2008-06-01 00:00:00

  • Three-dimensional models and structure analysis of corynemycolyltransferases in Corynebacterium glutamicum and Corynebacterium efficiens.

    abstract::The corynemycolyltransferase proteins were identified from Corynebacterium glutamicum and Corynebacterium efficiens genomes using computational tools available in the public domain. Three-dimensional models were constructed for corynemycolyltransferases based on the crystal structures of related mycolyltransferases in...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Adindla S,Guruprasad K,Guruprasad L

    更新日期:2004-06-01 00:00:00

  • Interaction of three new tetradentates Schiff bases containing N2O2 donor atoms with calf thymus DNA.

    abstract::Interaction of 1,3-bis(2-hydroxy-benzylidene)-urea (H2L1), 1,3-bis(2-hydroxy-3-methoxy-benzylidene)-urea (H2L2) and 1,3-bis(2-hydroxy-3-methoxy-benzylidene)-urea nickel(II) (NiL2) with calf-thymus DNA were investigated by UV-vis absorption, fluorescence emission and circular dichroism (CD) spectroscopy as well as cycl...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ajloo D,Shabanpanah S,Shafaatian B,Ghadamgahi M,Alipour Y,Lashgarbolouki T,Saboury AA

    更新日期:2015-01-01 00:00:00

  • Structural characteristics and prebiotic effects of Semen coicis resistant starches (type 3) prepared by different methods.

    abstract::In order to investigate the effects of different preparation methods on Semen coicis resistant starch, the structural properties and prebiotic effects of autoclaving treatment-purified S. coicis resistant starch (GP-SRS3), ultrasonic-autoclaving treatment-purified S. coicis resistant starch (UP-SRS3), enzyme-autoclavi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Bao C,Zeng H,Zhang Y,Zhang L,Lu X,Guo Z,Miao S,Zheng B

    更新日期:2017-12-01 00:00:00

  • High selective purification of IgY from quail egg: Process design and quantification of deep eutectic solvent based ultrasound assisted liquid phase microextraction coupled with preparative chromatography.

    abstract::The present research investigates on task-specific deep eutectic solvents (TDES) based aqueous two-phase extraction and purification of immunoglobulins by chromatography from quail egg. The synthesis of TDES was accomplished with quaternary ammonium salt as hydrogen bond acceptor (HBA) and glycerol as hydrogen bond do...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Balaraman HB,Rathnasamy SK

    更新日期:2020-03-01 00:00:00

  • Viscosity of alpha-crystallin solutions.

    abstract::Accommodation in the mammalian lens requires flexure of lens fibres and some redistribution of their contents involving limited viscous flow. Shear-dependent viscosity of bovine alpha-crystallin solutions was determined with the Contraves Low-Shear Rheometer between 4.4 and 347 mg ml(-1), and at 15.5, 25, 30, and 37 d...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Tiffany JM,Koretz JF

    更新日期:2002-06-18 00:00:00

  • Dacarbazine as a minor groove binder of DNA: Spectroscopic, biophysical and molecular docking studies.

    abstract::A detailed investigation on the mode of action and binding mechanism of a potent anticancer drug, 5-(3,3-dimethyl-1-triazeno)-imidazole-4-carboxamide (DCR) with calf thymus DNA (ctDNA) was carried out. UV-vis and fluorescence spectrophotometry suggested the formation of complex between DCR and ctDNA. The binding const...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Ahmad I,Ahmad M

    更新日期:2015-08-01 00:00:00

  • Heme degradation and iron release of hemoglobin and oxidative stress of lymphocyte cells in the presence of silica nanoparticles.

    abstract::Silica nanoparticles (SiO2 NPs) have been widely used in the medical and food sciences. However, their toxic effects against bio-macromolecules and cells are not well understood. The present study was aimed to investigate the adverse effects of fabricated SiO2 NPs on the human hemoglobin (Hb) by FTIR, CD, fluorescence...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Azimipour S,Ghaedi S,Mehrabi Z,Ghasemzadeh SA,Heshmati M,Barikrow N,Attar F,Falahati M

    更新日期:2018-10-15 00:00:00

  • Evaluation of multifunctional properties of gallic acid crosslinked Poly (vinyl alcohol)/Tragacanth Gum blend films for food packaging applications.

    abstract::The natural polymer Tragacanth Gum is less explored as a supporting matrix, there are very less studies conducted using this polymer in literature. So the present study aims to explore the consequences of different weight percent (wt.%) of gallic acid (GA) on physicochemical properties of Poly (vinyl alcohol)/Tragacan...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Goudar N,Vanjeri VN,Dixit S,Hiremani V,Sataraddi S,Gasti T,Vootla SK,Masti SP,Chougale RB

    更新日期:2020-04-28 00:00:00

  • A new water-soluble polysaccharide from Echinops pungens Trautv roots. Part I. Isolation, purification, characterization and antioxidant activity.

    abstract::EPPS, a new heteropolysaccharide from Echinops pungens roots, was extracted by warm water (70 °C) and fortuitously purified using column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Total carbohydrate content, specific optical rotation and molecular mass of EPPS were 98.3%, -88.2 and 65.7 kDa, respective...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Hoseiniyan Benvidi SM,Jahanbin K

    更新日期:2020-10-15 00:00:00

  • Amorphous aggregate adducts of linker histone H1 turn highly immunologic in the cancers of oesophagus, stomach, gall bladder and ovary.

    abstract::Hyperglycaemic influence on carcinogenesis and tumour progression is emerging as a link between diabetes and cancer. This work establishes the disturbed structural integrity of nucleosomal linker histone H1 by methyglyoxal (MG) and then correlates the role of modified H1 in the auto-immunopathogenesis of multiple canc...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Mir AR,Moinuddin,Habib S

    更新日期:2017-03-01 00:00:00

  • Extraction and characterization of pectin from Premna microphylla Turcz leaves.

    abstract::In this work, response surface methodology (RSM) and microwave pretreatment were used to extract pectin from Premna microphylla Turcz leaves (PMTL). The process variables were optimized by the isovariant central composite design to improve the pectin extraction yield. The optimum conditions obtained were as follows, e...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章


    authors: Lu J,Li J,Jin R,Li S,Yi J,Huang J

    更新日期:2019-06-15 00:00:00