当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Structural-functional insights and studies on saccharide binding of Sophora japonica seed lectin.

Structural-functional insights and studies on saccharide binding of Sophora japonica seed lectin.

Abstract：

:Functional and conformational transitions of the Sophora japonica seed lectin (SJL) were studied in detail using bioinformatics and biophysical tools. Homology model of the lectin displayed all the characteristics of the legume lectin monomer and the experimental observations correlated well with the structural information. In silico studies were performed by protein-ligand docking, calculating the respective binding energies and the residues involved in the interactions were derived from LigPlot(+) analysis. Fluorescence titrations showed three times higher affinity of T-antigen disaccharide than N-acetyl galactosamine (GalNAc) towards SJL indicating extended sugar binding site of the lectin. Thermodynamic parameters of T-antigen binding to SJL indicated the process to be endothermic and entropically driven while those of GalNAc showed biphasic process. SDS-PAGE showed post-translationally modified homotetrameric species of the lectin under native conditions. In presence of guanidine hydrochloride (0.5-5.0M), the tetramer first dissociated into dimers followed by unfolding of the protein as indicated by size exclusion chromatography, fluorescence and CD spectroscopy. Different structural rearrangements were observed during thermal denaturation of SJL at physiological pH 7.2, native pH 8.5 and molten globule inducing pH 1.0. Topological information revealed by solute quenching studies at respective pH indicated differential hydrophobic environment and charge density around tryptophan residues.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Yadav P,Shahane G,Ramasamy S,Sengupta D,Gaikwad S

doi

10.1016/j.ijbiomac.2016.05.047

subject

Has Abstract

pub_date

2016-10-01 00:00:00

pages

75-84

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(16)30458-5

journal_volume

91

pub_type

杂志文章

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