Abstract：
:A specific HPLC-MS/MS (High-Performance Liquid Chromatography with tandem Mass Spectrometry) method was developed and validated for simultaneous determination of several monosaccharides in three kinds Osmanthus fragrans Lour. After extraction, separation, protein removal, pigment removal and hydrolysis, monosaccharides was finally obtained from Osmanthus fragrans Lour. Positive ion mode detection and Multiple Reaction Monitoring (MRM) mode were used for quantitative analysis by PMP pre-column derivatization and Electrospray Ionization (ESI). Analysis and content determination of 6 monosaccharide components in 3 kinds of Osmanthus fragrans Lour. The HPLC separation was achieved on a Shim-pack VP-ODS6022748 (150 L × 2.0) with gradient elution at a flow rate of 0.2 ml/min in a run time of 40 min, and the mobile phase was acetonitrile-5 mmol/L ammonium acetate. PMP derivatization in HPLC-MS/MS can accurately measure Osmanthus fragrans Lour. mannose (Man), ribose (Rib), rhamnose (Rha), galacturonic acid (Gal UA), glucose (Glu), galactose (Gal), xylose (Xyl), fucose (Fuc). The results showed that HPLC-MS/MS pre-column derivatization method was simple and rapid, with small measurement error, but high sensitivity and good repeatability. The analysis of monosaccharide components in polysaccharide components has important practical significance.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Fan B,Li T,Song X,Wu C,Qian Cdoi
10.1016/j.ijbiomac.2018.12.033subject
Has Abstractpub_date
2019-03-15 00:00:00pages
221-231eissn
0141-8130issn
1879-0003pii
S0141-8130(18)35983-Xjournal_volume
125pub_type
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