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ESI-MS measurements for the equilibrium constants of copper(II)-insulin complexes.

Abstract：

:Trace elements regulate many biological reactions in the body. Copper(II) is known as one of trace elements and capable of binding to proteins. Insulin is a blood glucose-lowering peptide hormone and it is secreted by the pancreatic β-cells. In this study, Cu(II)-insulin complexes were investigated by using ESI-MS method. Insulin molecule gives ESI-MS peaks at +4, +5, +6 and +7 charged states. Cu(II)-insulin complexes can be monitored and quantified on the ESI-MS spectra as the shifted peaks according to insulin peaks. The solutions of Cu(II)-insulin complexes at different pHs and mole ratios of Cu(II) ions to insulin molecule were measured on the ESI-MS. The highest complex formation ratio for Cu(II)-insulin were found at pH 7. The multiple bindings of Cu(II) ions to insulin molecule was observed. The formation equilibrium constants of Cu(II)-insulin complexes were calculated as Kf1: 3.34 × 104, Kf2: 2.99 × 104, Kf3: 7.00 × 103 and Kf4:2.86 × 103. The specific binding property of Cu(II) ions was controlled by using different spray ion sources including electrospray and nano-electrospray. The binding property of Cu(II) also investigated by MS/MS fragmentation. It was concluded from the ESI-MS measurements that Cu(II) ion has a high affinity to insulin molecules to form stable complexes.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Gülfen M,Özdemir A,Lin JL,Chen CH

doi

10.1016/j.ijbiomac.2018.01.150

subject

Has Abstract

pub_date

2018-06-01 00:00:00

pages

188-196

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)34349-0

journal_volume

112

pub_type

杂志文章

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