Abstract：
:CD117/c-kit, a tyrosine kinase receptor, plays a critical role in hematopoiesis, pigmentation, and fertility. The overexpression and activation of c-kit are thought to promote tumor growth and have been reported in various cancers, including leukemia, glioblastoma and mastocytosis. To disrupt the SCF/c-kit signaling axis in cancer, we generated a c-kit antagonist human antibody (NN2101) that binds to domain 2/3 of c-kit. This completely blocked the SCF-mediated phosphorylation of c-kit and inhibited TF-1 cell proliferation, erythroleukemia. In addition, the examination of binding affinity using surface plasmon resonance (SPR) assay showed that NN2101 can bind to c-kit of monkeys (KD = 2.92 × 10-10 M), rats (KD = 1.68 × 10-6 M), mice (KD = 11.5 × 10-9 M), and humans (KD = 2.83 × 10-12 M). We showed that NN2101 does not cause antibody-dependent cell-mediated cytotoxicity and complement-dependent cytotoxicity. The immunogenicity of NN2101 was similar to that of bevacizumab. Furthermore, the crystal structure of NN2101 Fab was determined and the structure of NN2101 Fab:c-kit complex was modeled. Structural information, as well as mutagenesis results, revealed that NN2101 can bind to the SCF-binding regions of c-kit. Collectively, we generated a c-kit neutralizing human antibody (NN2101) for the treatment of erythroleukemia and characterized its biophysical properties. NN2101 can potentially be used as a therapeutic antibody to treat different cancers.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Kim JO,Kim HN,Kim KH,Baek EJ,Park JY,Ha K,Heo DR,Seo MD,Park SGdoi
10.1016/j.ijbiomac.2020.05.045subject
Has Abstractpub_date
2020-09-15 00:00:00pages
66-78eissn
0141-8130issn
1879-0003pii
S0141-8130(20)33190-1journal_volume
159pub_type
杂志文章abstract：:Ordered porous sodium alginate/graphene oxide (SAGO) aerogel was fabricated by in situ crosslinking and freeze-drying method. GO, as reinforcing filler, can be easily incorporated with SA matrix by self-assembly via hydrogen bonding interaction. Compared with pure SA aerogel, the as-prepared SAGO exhibited excellent m...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2015.11.061
更新日期：2016-02-01 00:00:00
abstract：:Non-starch polysaccharides are considered the main bioactive ingredients in kiwifruits. In order to well understand the chemical structures and antioxidant activities of non-starch polysaccharides from different varieties of kiwifruits (KPSs), the physicochemical characteristics and antioxidant activities of KPSs extr...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.06.142
更新日期：2019-09-01 00:00:00
abstract：:Electrospinning of cellulose acetate with appropriated solvent system is the most straightforward method for fabricating micro- and nanofibers. To simultaneously and effectively remove both cationic and anionic dyes, a novel cost-effective multifunctional cellulose acetate (CA) fibers membrane was prepared by electros...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.04.249
更新日期：2020-05-03 00:00:00
abstract：:Thermococcus gammatolerans is anaerobic euryarchaeon which grows optimally at 88 °C and its genome encodes a family B DNA polymerase (Tga PolB). Herein, we cloned the gene of Tga PolB, expressed and purified the gene product, and characterized the enzyme biochemically. The recombinant Tga PolB can efficiently synthesi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.204
更新日期：2020-08-01 00:00:00
abstract：:Wheat straw was cooked under different pulping processes: Soda (100°C, 7% NaOH, 150min), Kraft (170°C, 16% alkalinity, 25% sulfidity, 40min) and Organosolv (210°C, 60% ethanol, 60min). Once the pulps were obtained, lignocellulose nanofibers (LCNF) were isolated by mechanical process and TEMPO-mediated oxidation follow...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.08.019
更新日期：2016-11-01 00:00:00
abstract：:During the last few decades, there has been evident growth in the development and fabrication of nano structured materials due to their wide application for different fields of material science and engineering. Among all these materials, natural polymer-based hydrogels such as polysaccharides, proteins, lipids etc. ar...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2020.09.012
更新日期：2020-12-01 00:00:00
abstract：:In the present work, chitosan physicochemical transformations that occur during high energy ball milling are investigated and correlated with adsorption capacity of organic pollutants (using azo-dye reactive red 2 as molecular probe). Experimental results reveal that chitosan ball milled for 1 h shows a 70% increase o...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.01.171
更新日期：2020-04-01 00:00:00
abstract：:Previous investigations on the role of the polymerase in the synthesis of poly-3-hydroxybutyrate (PHB) are reviewed, and the results from earlier in vitro studies on the activity and selectivity of the polymerase of Alcaligenes eutrophus are discussed. In the present study the effect of glycerol on stabilizing the pol...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(99)00015-x
更新日期：1999-06-01 00:00:00
abstract：:Development of bio-nanoreinforcement is gaining momentum worldwide nowadays since it contributes much to the green materials era. This paper presents a proposal for reusing the agro waste to produce cellulose nanofibres in an ecofriendly way. The present work aims to isolate cellulose nanofibres (CNF) from pineapple l...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.03.134
更新日期：2019-06-15 00:00:00
abstract：:Rheological, thermal and microstructural properties of wheat bran sourdough (WBS) containing different levels of microbial exopolysaccharide (EPS) extracted from Lactobacillus plantarum were investigated. All sourdough samples showed pseudoplastic behavior and the highest apparent viscosity was achieved at 1.5% EPS. T...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.03.149
更新日期：2020-07-01 00:00:00
abstract：:Cellulose was modified by formic acid to prepare formyl cellulose (FC). The amount of formyl groups in FC was adjusted by controlling the reaction time, reaction temperature, and formic acid concentration. Then, FC was used to reinforce polylactic acid (PLA) films prepared by solution casting. Scanning electron micros...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2021.01.029
更新日期：2021-01-09 00:00:00
abstract：:Microwave synthesis of starch-graft-poly(ethylacrylate) was optimized to obtain efficient Zn(II) adsorbent that has been characterized using IR, XRD and SEM analyses. Using the sample, adsorption of Zn(II) was studied as a function of pH, sorbent dose, initial Zn(II) concentration, % grafting, temperature and ionic st...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2010.05.023
更新日期：2010-10-01 00:00:00
abstract：:In the present study, we have fabricated a novel chitosan based nanocomposite (g-C3N4/Ag3PO4/CS) containing g-C3N4 and Ag3PO4 nanoparticles. The fabricated nanocomposite was characterized successfully and used as a catalyst for photocatalytic degradation of ciprofloxacin (CIP) under visible light. The TEM results reve...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.08.133
更新日期：2020-12-01 00:00:00
abstract：:The interaction of nanoparticles (NPs) with proteins is a topic of high relevance for the medical application of nanomaterials. In this study, a comprehensive investigation was performed to clarify the binding mechanism, adsorption isotherms and kinetics of the interaction between silver nanoparticles (AgNPs) and tryp...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.03.172
更新日期：2018-07-15 00:00:00
abstract：:This article is devoted to synthesis of a new magnetic interphase palladium catalyst that has been immobilized on chitosan-biguanidine coated-magnetic Fe3O4 nanoparticles [Pd(0/II)/CS-bigua@Fe3O4]. Such surface functionalization of magnetic particles is a promising method to bridge the gap between heterogeneous and ho...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2018.02.120
更新日期：2018-07-01 00:00:00
abstract：:The effects of metalation process by inorganic compounds containing Au, Pt and Ru on protein structure and on conformation and flexibility of the residues involved in the metal compound binding have been here investigated by analysing 204 structures of protein/metallodrug adducts and the corresponding metal-free forms...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.07.053
更新日期：2020-11-15 00:00:00
abstract：:A series of 4- substituted sampangine derivatives (4-aminoalkylaminosampangine Ar-NH(CH2)nNR1R2) has been designed, synthesized, and tested for their ability to inhibit acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and β-myloid (Aβ) aggregation. The synthetic compounds exhibited high AChE inhibitory activi...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.10.157
更新日期：2018-02-01 00:00:00
abstract：:Pancreatic lipase (PL) is a primary lipase critical for triacylglyceride digestion in humans and is considered as a promising target for the treatment of obesity. Although the current synthetic drugs available for treating obesity have been demonstrated to be effective in inhibiting PL, their prolonged usage results i...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.01.150
更新日期：2017-05-01 00:00:00
abstract：:Although synthetic antioxidant food additives are widely used in a variety of food products, some of them are suspected of having a noxious effect on human health. As a consequence, much research attention has been focused on developing natural antioxidant compounds from plants. Riang (Parkia timoriana (DC.) Merr.) is...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.079
更新日期：2020-12-01 00:00:00
abstract：:In this study the extraction of hot water soluble polysaccharides (HWSPs) from Cymbopogon citratus using hot water decoction was discussed. Response surface methodology (RSM) based on a three level, three variable central composite rotatable design (CCRD), was employed to obtain best possible combination of extraction...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2014.01.033
更新日期：2014-04-01 00:00:00
abstract：:Serine protease inhibitors, inhibit serine proteases either partially or completely after forming complexes with their respective proteases. Protease actions are significant for many physiological pathways found in living forms and any anomalies may lead to numerous physiological complications. Each cell or organism h...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章,评审
doi：10.1016/j.ijbiomac.2017.09.115
更新日期：2018-02-01 00:00:00
abstract：:This paper introduces a cheap simple MWCNTs@paper biosensor for the detection of CA19-9, which is a biomarker of pancreatic cancer. By adding the CA19-9 antibody to the surface of MWCNTs which are deposited on the microporous filter paper, the correlation between the concentration of CA19-9 and resistance of biosensor...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.09.176
更新日期：2020-02-01 00:00:00
abstract：:Salecan is a novel water-soluble extracellular β-glucan and suitable for the hydrogel preparation due to its excellent physicochemical and biological properties. The present article describes the fabrication and characterization of a pH/magnetic field-driven hydrogel based on salecan-g-poly(vinylacetic acid-co-2-hydro...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.12.018
更新日期：2018-03-01 00:00:00
abstract：:A newly isolated bacterium producing 55.5 U/mL keratinase on feather meal minimal medium was identified as Ochrobactrum intermedium. Optimization of process parameters by one-variable-at-a-time (OVAT) approach (substrate concentration 0.5% w/v, inoculum size 5% w/v, pH 7.0, 200 rpm for 96 h at 40 °C) resulted in 2.1-f...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.10.260
更新日期：2021-01-01 00:00:00
abstract：:Nanoparticles are produced by means of polyelectrolyte complexation (PEC) of oppositely charged polycationic chitosan (CH) with polyanionic polysaccharide extracted from Sterculia striata exudates (rhamnogalacturonoglycan (RG)-type polysaccharide). The nanoparticles formed with low-molar-mass CH are larger than those ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2016.03.070
更新日期：2016-07-01 00:00:00
abstract：:In recent days, the green synthesized nanomagnetic biocomposites have been evolved with tremendous potential as the future catalysts. This has encouraged us to design and synthesis of a novel Au NPs immobilized pectin modified magnetic nanoparticles (Fe3O4/Pectin/Au). It was meticulously characterized using advanced a...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2020.09.102
更新日期：2020-11-15 00:00:00
abstract：:Salecan is a linear microbial polysaccharide obtained from the fermentation of Agrobacterium sp. ZX09. Salecan is suitable for the preparation of hydrogels for biomedical applications, given its prominent physicochemical and biological properties. Herein, we described a novel microwave-assisted polymerized composite h...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2019.05.191
更新日期：2019-08-15 00:00:00
abstract：:Contractile filaments in skeletal muscle are moved by less than 2nm for each ATP used. If just one cross-bridge is attached to each thin filament at any instant then this distance represents the fundamental myosin cross-bridge step size (i.e. the distance one cross-bridge moves a thin filament in one ATP-splitting cyc...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/s0141-8130(03)00047-3
更新日期：2003-09-01 00:00:00
abstract：:The formation of heteroprotein coacervates obtained by the interaction of ovalbumin (Ova) and lysozyme (Lys) was investigated using turbidimetric analysis and the zeta potential at different protein ratios, pH values and concentrations of NaCl. The complexes were formed over a wide pH range with a 1:1 (Ova:Lys) ratio ...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2017.08.132
更新日期：2018-01-01 00:00:00
abstract：:Biodegradable flocculants of Gum ghatti (Gg) with acrylamide (AAm) were prepared through graft co-polymerization technique using potassium persulphate (KPS)-ascorbic acid (ABC) redox pair as initiator and N,N'-methylene-bis-acrylamide (MBA) as a crosslinker. Gg-cl-poly(AAm) was found to exhibit pH and temperature resp...
journal_title：International journal of biological macromolecules
pub_type： 杂志文章
doi：10.1016/j.ijbiomac.2013.03.045
更新日期：2013-07-01 00:00:00
