当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Rapid self-association of highly amyloidogenic H-fragments of insulin: Experiment and molecular dynamics simulations.

Rapid self-association of highly amyloidogenic H-fragments of insulin: Experiment and molecular dynamics simulations.

Abstract：

:The so-called 'H-fragment' of insulin is an extremely amyloidogenic double chain peptide consisting of the N-terminal parts of A-chain and B-chain linked by a disulfide bond between Cys-7A and Cys-7B. Here, we conduct a detailed investigation of the self-association behavior of H-fragment monomers into amyloid-like fibrils using kinetic assays, infrared spectroscopy, circular dichroism (CD), atomic force microscopy (AFM) and molecular dynamics (MD) simulations. Unlike the intact predominantly α-helical insulin, H-fragment remains in a disordered state in aqueous solutions. Its aggregation accelerates with acidification of the environment leading, at pH 1.9, to the formation of thin and structurally homogenous fibrils with the infrared features typical for parallel β-sheet conformation. According to time-lapse AFM morphological analysis both secondary nucleation and fragmentation are involved in later stages of H-fibrils' self-assembly. Based on the low nucleation order (two) obtained from the global fitting of kinetic data, realistic all-atom MD simulations of pairs of interacting H-fragment monomers were subsequently carried out. The molecular self-association scenario emerging from these simulations implicates the intrinsic conformational instability of H-monomer in its tendency to aggregate and form intermolecular β-sheet structure. Our findings provide the new mechanistic context for studies of insulin misfolding and aggregation.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Dec R,Koliński M,Kouza M,Dzwolak W

doi

10.1016/j.ijbiomac.2020.02.153

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

894-903

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)30852-7

journal_volume

150

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Structures, properties and application of alginic acid: A review.

    abstract：:Alginic acid is a natural polysaccharide, which has been widely concerned and applied due to its excellent water solubility, film formation, biodegradability and biocompatibility. This paper briefly describes the source, properties, structure and application of sodium alginate by summarizing and analyzing the current ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2020.06.180

    authors： Guo X,Wang Y,Qin Y,Shen P,Peng Q

    更新日期：2020-11-01 00:00:00

  • Perspectives on progressive strategies and recent trends in the production of recombinant human factor VIII.

    abstract：:Factor VIII (fVIII), a glycoprotein cofactor, plays a crucial role in the intrinsic blood coagulation pathway. As one of the most essential blood clotting factors known today, fVIII is the largest and most complex commercialized therapeutic protein used in the treatment of hemophilia A, an X-linked recessive disorder....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2018.07.164

    authors： Mannully ST,L N R,Pulicherla KK

    更新日期：2018-11-01 00:00:00

  • Effect of hydroxypropyl-β-cyclodextrin on the bounding of salazosulfapyridine to human serum albumin.

    abstract：:The supramolecular interaction between salazosulfapyridine (SASP) and hydroxypropyl-β-cyclodextrin (HP-β-CD), as well as the influence of HP-β-CD on SASP's binding to human serum albumin (HSA), were investigated. Phase-solubility studies indicate that the HP-β-CD/SASP inclusion complex was formed at a 1:1 host-guest s...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.07.033

    authors： Tang P,Tang B,Wang Q,Xu K,Xiong X,Li H

    更新日期：2016-11-01 00:00:00

  • Biochemical characterization and molecular docking of cloned xylanase gene from Bacillus subtilis RTS expressed in E. coli.

    abstract：:This study employed mesophilic Bacillus subtilis RTS strain isolated from soil with high xylanolytic activity. A 642 bp (xyn) xylanase gene (GenBank accession number MT677937) was extracted from Bacillus subtilis RTS and cloned in Escherichia coli BL21 cells using pET21c expression system. The cloned gene belongs to g...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.12.001

    authors： Saleem A,Waris S,Ahmed T,Tabassum R

    更新日期：2021-01-31 00:00:00

  • Exopolysaccharides production by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12: Optimization of fermentation variables and characterization of structure and bioactivities.

    abstract：:In this study, the most important variables (incubation temperature, fermentation time and yeast extract concentration) responsible for the exopolysaccharides (EPSs) production by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12 were screened. The EPSs synthesize by LA5, BB12, and their co...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.084

    authors： Amiri S,Rezaei Mokarram R,Sowti Khiabani M,Rezazadeh Bari M,Alizadeh Khaledabad M

    更新日期：2019-02-15 00:00:00

  • Effective adsorptive removal of reactive dyes by magnetic chitosan nanoparticles: Kinetic, isothermal studies and response surface methodology.

    abstract：:In the present study, magnetic chitosan (MC) was synthesized, characterized, and used as an efficient adsorbent for the removal of Reactive Red 141 (RR-141) and Reactive Yellow 14 (RY-14) dyes. Synthesized magnetic chitosan nanoparticles were used for characterization with the help of scanning electron microscope (SEM...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.07.042

    authors： Jaafari J,Barzanouni H,Mazloomi S,Amir Abadi Farahani N,Sharafi K,Soleimani P,Haghighat GA

    更新日期：2020-12-01 00:00:00

  • Glucose-sensitive delivery of metronidazole by using a photo-crosslinked chitosan hydrogel film to inhibit Porphyromonas gingivalis proliferation.

    abstract：:A novel glucose-sensitive drug delivery system with controlled release of metronidazole was synthesized by using biocompatible photo-crosslinked chitosan hydrogel film. Specifically, methacrylic anhydride, as photosensitive substance, was firstly grafted on chitosan molecular chains and then UV irradiation was applied...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.202

    authors： Liu J,Xiao Y,Wang X,Huang L,Chen Y,Bao C

    更新日期：2019-02-01 00:00:00

  • Electrospraying of environmentally sustainable alginate microbeads for cosmetic additives.

    abstract：:Polymer microbeads (MBs) for scrubbing additives have generally been prepared from non-biodegradable synthetic polymers. The worldwide pollution of the marine ecosystem by microplastics urgently demands novel environment-friendly MBs. In this study, Ca-alginate MBs were fabricated by electrospraying an aqueous alginat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.058

    authors： Bae SB,Nam HC,Park WH

    更新日期：2019-07-15 00:00:00

  • Renoprotective effect of ramulus mori polysaccharides on renal injury in STZ-diabetic mice.

    abstract：:Recently, increasing evidences have suggested that inflammatory stress is markedly occurred in the impaired tissue. Thus, the streptozotocin (STZ)-induced diabetic mice were used to investigate the potential renoprotective effect of ramulus mori polysaccharides (RMP) and to discuss the underlying mechanism. The result...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.09.022

    authors： Guo C,Liang T,He Q,Wei P,Zheng N,Xu L

    更新日期：2013-11-01 00:00:00

  • Date palm (Phoenix dactylifera L.) fruit's polyphenols as potential inhibitors for human amylin fibril formation and toxicity in type 2 diabetes.

    abstract：BACKGROUND:β-Cell death is the key feature of type 2 diabetes mellitus (T2DM). The misfolding of human Islet Amyloid Polypeptide (hIAPP) is regarded as one of the causative factors of T2DM. Recent studies suggested that a diet based on date fruits presents various health benefits, as these fruits are naturally enriched...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.08.080

    authors： Chaari A,Abdellatif B,Nabi F,Khan RH

    更新日期：2020-12-01 00:00:00

  • Use of glyoxyl-agarose immobilized ficin extract in milk coagulation: Unexpected importance of the ficin loading on the biocatalysts.

    abstract：:A protein extract obtained from fig tree (Ficus carica) latex containing ficin activity was immobilized on glyoxyl agarose. Different biocatalyst loadings were used (3, 10, 30 and 85 mg/g). When casein was used as substrate, the expressed activities were 60%, 58%, 41% and 14%, respectively, very likely due to casein d...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.12.140

    authors： Siar EH,Morellon-Sterling R,Zidoune MN,Fernandez-Lafuente R

    更新日期：2020-02-01 00:00:00

  • The first complete mitochondrial genome from the family Hygrophoraceae (Hygrophorus russula) by next-generation sequencing and phylogenetic implications.

    abstract：:Hygrophorus russula (Schaeff.) Kauffman is an edible ectomycorrhizal fungus that is widely distributed in the world. In this study, the mitogenome of H. russula was sequenced and assembled. The mitogenome of H. russula is composed of circular DNA molecules, with a total size of 55,769 bp. Further analysis indicated th...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.091

    authors： Li Q,Wang Q,Jin X,Chen Z,Xiong C,Li P,Zhao J,Huang W

    更新日期：2019-02-01 00:00:00

  • Improved pullulan production by a mutant of Aureobasidium melanogenum TN3-1 from a natural honey and capsule shell preparation.

    abstract：:Aureobasidium melanogenum TN3-1 isolated from a natural honey was a highly genome-duplicated yeast-like fungal strain and a very high pullulan producer. In this study, simultaneous removal of both duplicated AMY1 genes encoding α-amylase and duplicated PKS1 genes responsible for melanin biosynthesis in A. melanogenum ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.08.264

    authors： Chen L,Chi Z,Liu GL,Xue SJ,Wang ZP,Hu Z,Chi ZM

    更新日期：2019-12-01 00:00:00

  • Chitosan coated synergistically engineered nanoemulsion of Ropinirole and nigella oil in the management of Parkinson's disease: Formulation perspective and In vitro and In vivo assessment.

    abstract：:The research presented aims at developing Ropinirole hydrochloride (RHCl) nanoemulsion (NE) with nigella oil for Parkinson's disease (PD). In silico study was done to explore interactions of ropinirole and thymoquinone at receptor site (TNF-α and NFK-β). Ropinirole and Thymoquinone forms a hydrogen bond with residue A...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.207

    authors： Nehal N,Nabi B,Rehman S,Pathak A,Iqubal A,Khan SA,Yar MS,Parvez S,Baboota S,Ali J

    更新日期：2021-01-15 00:00:00

  • Structural characteristics and prebiotic effects of Semen coicis resistant starches (type 3) prepared by different methods.

    abstract：:In order to investigate the effects of different preparation methods on Semen coicis resistant starch, the structural properties and prebiotic effects of autoclaving treatment-purified S. coicis resistant starch (GP-SRS3), ultrasonic-autoclaving treatment-purified S. coicis resistant starch (UP-SRS3), enzyme-autoclavi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.07.091

    authors： Bao C,Zeng H,Zhang Y,Zhang L,Lu X,Guo Z,Miao S,Zheng B

    更新日期：2017-12-01 00:00:00

  • Effect of triglyceride on complexation between starch and fatty acid.

    abstract：:This study aimed to reveal the effect of corn oil on formation of complex between rice starch and fatty acids, including lauric acid (C12:0), myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0). The RVA, XRD, DSC and CLSM analysis confirmed formation of starch-fatty acid complex but the starch-trigly...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.11.072

    authors： Li X,Luo S,Hou Y,Liu Y,Hu X,Liu C

    更新日期：2020-07-15 00:00:00

  • Thermal unfolding of apo- and holo-enolase from Saccharomyces cerevisiae: different mechanisms, similar activation enthalpies.

    abstract：:Yeast enolase is stabilized by its natural cofactor Mg(2+). This stabilization is ascribed to the reduced subunit dissociation of the holoprotein. Nevertheless, how Mg(2+) alters the unfolding mechanism has yet to be fully characterized. Here, we investigate the role of Mg(2+) in the denaturation mechanism and unfoldi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.07.021

    authors： Moreno-Vargas LM,Carrillo-Ibarra N,Arzeta-Pino L,Benítez-Cardoza CG

    更新日期：2011-12-01 00:00:00

  • NMR studies of succinoglycan repeating-unit octasaccharides from Rhizobium meliloti and Agrobacterium radiobacter.

    abstract：:Complete 1H and 13C-nuclear magnetic resonance assignments have been obtained for the octasaccharide repeating units of the bacterial polysaccharide succinoglycan from Rhizobium meliloti Rm1021 and Agrobacterium radiobacter NCIB 11883. The assignments were used to determine the locations of the O-succinyl and O-acetyl...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(96)81846-0

    authors： Chouly C,Colquhoun IJ,Jodelet A,York G,Walker GC

    更新日期：1995-12-01 00:00:00

  • Optimization, characterization and evaluation of chitosan-tailored cubic nanoparticles of clotrimazole.

    abstract：:The present study deals with improvement of the mucoadhesive properties of monoolein based cubic nanoparticles by incorporating chitosan. Chitosan-tailored cubic nanoparticles were prepared by thin film hydration followed by ultrasonication employing clotrimazole as model drug. The effect of Pluronic F127 fraction and...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.10.065

    authors： Verma P,Ahuja M

    更新日期：2015-02-01 00:00:00

  • Biochemical and molecular characterization of a novel dye-decolourizing peroxidase from Raoultella ornithinolytica OKOH-1.

    abstract：:The increase in industrial demand for peroxidases has necessitated the search for novel peroxidase with excellent industrial versatility. Raoultella ornithinolytica OKOH-1 is a new ligninolytic bacteria with peroxidase production potential. However, there is paucity of information on characterization of peroxidase fro...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.10.045

    authors： Falade AO,Mabinya LV,Okoh AI,Nwodo UU

    更新日期：2019-01-01 00:00:00

  • Isolation and identification of chitin in the black coral Parantipathes larix (Anthozoa: Cnidaria).

    abstract：:Until now, there is a lack of knowledge about the presence of chitin in numerous representatives of corals (Cnidaria). However, investigations concerning the chitin-based skeletal organization in different coral taxa are significant from biochemical, structural, developmental, ecological and evolutionary points of vie...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2012.04.016

    authors： Bo M,Bavestrello G,Kurek D,Paasch S,Brunner E,Born R,Galli R,Stelling AL,Sivkov VN,Petrova OV,Vyalikh D,Kummer K,Molodtsov SL,Nowak D,Nowak J,Ehrlich H

    更新日期：2012-07-01 00:00:00

  • Comparison of laccase-catalyzed cross-linking of organosolv lignin and lignosulfonates.

    abstract：:Lignin, an underutilized by-product from chemical pulping of wood, can be modified enzymatically through oxidation by laccase. However, little is known about the molecular details surrounding the cross-linking which is a result of the oxidation. To reduce this lack of knowledge, we used oxygen consumption rate data, p...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.07.061

    authors： Gillgren T,Hedenström M,Jönsson LJ

    更新日期：2017-12-01 00:00:00

  • Chromium (VI) detoxification by oxidation and flocculation of exopolysaccharides from Arthrobacter sp. B4.

    abstract：:The exopolysaccharides from Arthrobacter sp. B4 (B4-EPS) exhibited an excellent chromium (VI) (Cr(VI)) removal capability without any pH adjustment, whereby 50mgL(-1) of Cr(VI) could be completely removed by 4gL(-1) of B4-EPS. The kinetics tests revealed that the first-order rate constant was 8.3×10(-5)s(-1) and the o...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.07.013

    authors： Li Y,Li Q,Fengying Y,Bao J,Hu Z,Zhu W,Zhao Y,Lin Z,Dong Q

    更新日期：2015-11-01 00:00:00

  • Effect of cross-linking on the dimensional stability and biocompatibility of a tailored 3D-bioprinted gelatin scaffold.

    abstract：:Biocompatible and biodegradable gelatin is a good candidate bioink for use in 3D bioprinting technologies, but viscous gelatin solution has a low printability. In order to improve the poor printability of gelatin, we optimized the rheological properties of gelatin solution. 3D gelatin scaffolds were then cross-linked ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.05.207

    authors： Choi DJ,Kho Y,Park SJ,Kim YJ,Chung S,Kim CH

    更新日期：2019-08-15 00:00:00

  • Activation and stabilization of Candida antarctica lipase B in choline chloride-glycerol-water binary system via tailoring the hydrogen-bonding interaction.

    abstract：:As a new generation of green solvent, the utilization of natural deep eutectic solvents (NADESs) in enzymatic reactions has attracted widespread academic and industrial interests. Especially, choline chloride-glycerol (C-Gly) has been extensively used in many green chemistry processes. In this study, a series of C-Gly...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.06.150

    authors： Nian B,Cao C,Liu Y

    更新日期：2019-09-01 00:00:00

  • Preparation and characterization of chitosan/gelatin/nanocrystalline cellulose/calcium peroxide films for potential wound dressing applications.

    abstract：:In order to improve mechanical properties and biomedical behavior of chitosan-based polymeric films, the effect of nanocrystalline cellulose (NCC) and calcium peroxide (CP) particles on the properties of polymeric films based on chitosan (CS) and gelatin (GL) were investigated in this study. The films were characteriz...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.159

    authors： Akhavan-Kharazian N,Izadi-Vasafi H

    更新日期：2019-07-15 00:00:00

  • WITHDRAWN: Nanomaterials Chemistry: Recent Developments and New Directions.

    abstract：:The Publisher regrets that this article is an accidental duplication of an article that has already been published in Int. J. Biol. Macromol, doi:10.1016/j.ijbiomac.2008.07.012. The duplicate article has therefore been withdrawn. ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2008.08.002

    authors： Kennedy JF

    更新日期：2008-08-19 00:00:00

  • Natural polypeptides-based electrically conductive biomaterials for tissue engineering.

    abstract：:Fabrication of an appropriate scaffold is the key fundamental step required for a successful tissue engineering (TE). The artificial scaffold as extracellular matrix in TE has noticeable role in the fate of cells in terms of their attachment, proliferation, differentiation, orientation and movement. In addition, chemi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2019.12.249

    authors： Vandghanooni S,Eskandani M

    更新日期：2020-03-15 00:00:00

  • Chemical characteristics and immuno-modulating activities of exo-biopolymers produced by Grifola frondosa during submerged fermentation process.

    abstract：:The immuno-modulating activities and chemical characteristics of exo-biopolymer (EX-GF) produced by a submerged mycelial culture of Grifola frondosa were studied. The EX-GF was fractionated into EX-GF-Fr.I, II, and III by Sephadex G-100 gel chromatography. Anti-complementary activity of EX-GF-Fr.III was highest (71.1%...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2007.02.012

    authors： Yang BK,Gu YA,Jeong YT,Jeong H,Song CH

    更新日期：2007-08-01 00:00:00

  • The benzene metabolite p-benzoquinone inhibits the catalytic activity of bovine liver catalase: A biophysical study.

    abstract：:The current communication reports the inhibitory effect of para-benzoquinone (p-BQ) on the structure and function of bovine liver catalase (BLC), a vital antioxidant enzyme. Both BLC and p-BQ were dissolved in respective buffers and the biophysical interaction was studied at physiological concentrations. For the first...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.11.044

    authors： Jena AB,Samal RR,Kumari K,Pradhan J,Chainy GBN,Subudhi U,Pal S,Dandapat J

    更新日期：2021-01-15 00:00:00