Rapid self-association of highly amyloidogenic H-fragments of insulin: Experiment and molecular dynamics simulations.

Abstract:

:The so-called 'H-fragment' of insulin is an extremely amyloidogenic double chain peptide consisting of the N-terminal parts of A-chain and B-chain linked by a disulfide bond between Cys-7A and Cys-7B. Here, we conduct a detailed investigation of the self-association behavior of H-fragment monomers into amyloid-like fibrils using kinetic assays, infrared spectroscopy, circular dichroism (CD), atomic force microscopy (AFM) and molecular dynamics (MD) simulations. Unlike the intact predominantly α-helical insulin, H-fragment remains in a disordered state in aqueous solutions. Its aggregation accelerates with acidification of the environment leading, at pH 1.9, to the formation of thin and structurally homogenous fibrils with the infrared features typical for parallel β-sheet conformation. According to time-lapse AFM morphological analysis both secondary nucleation and fragmentation are involved in later stages of H-fibrils' self-assembly. Based on the low nucleation order (two) obtained from the global fitting of kinetic data, realistic all-atom MD simulations of pairs of interacting H-fragment monomers were subsequently carried out. The molecular self-association scenario emerging from these simulations implicates the intrinsic conformational instability of H-monomer in its tendency to aggregate and form intermolecular β-sheet structure. Our findings provide the new mechanistic context for studies of insulin misfolding and aggregation.

journal_name

Int J Biol Macromol

authors

Dec R,Koliński M,Kouza M,Dzwolak W

doi

10.1016/j.ijbiomac.2020.02.153

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

894-903

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(20)30852-7

journal_volume

150

pub_type

杂志文章
  • Some physico-chemical properties of Prunus armeniaca L. gum exudates.

    abstract::The objectives of this paper were to investigate some physicochemical properties of Prunus armeniaca L. gum exudates (PAGE). PAGE had, on average, 66.89% carbohydrate, 10.47% uronic acids, 6.9% moisture (w.b.), 2.91% protein, 4% ash and 1.59% fat. PAGE was composed of monosaccharides including l-arabinose, d-galactose...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.09.068

    authors: Fathi M,Mohebbi M,Koocheki A

    更新日期:2016-01-01 00:00:00

  • Morphological and structural features of amylose spherocrystals of A-type.

    abstract::Amylose spherocrystals of A-type were grown by mixing ethanol with hot aqueous solutions of short chain amylose followed by slow cooling to 4 degrees C. The spherocrystals which had a diameter of the order of 10 microns were observed by scanning electron microscopy and analysed by X-ray diffraction. They were also cro...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/0141-8130(93)90021-d

    authors: Helbert W,Chanzy H,Planchot V,Buléon A,Colonna P

    更新日期:1993-06-01 00:00:00

  • Inner membrane complex 1l protein of Plasmodium falciparum links membrane lipids with cytoskeletal element 'actin' and its associated motor 'myosin'.

    abstract::The inner membrane complex (IMC) is a defining feature of apicomplexans comprising of lipid and protein components involved in gliding motility and host cell invasion. Motility of Plasmodium parasites is accomplished by an actin and myosin based glideosome machinery situated between the parasite plasma membrane (PPM) ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.12.239

    authors: Kumar V,Behl A,Kapoor P,Nayak B,Singh G,Singh AP,Mishra S,Kang TS,Mishra PC,Hora R

    更新日期:2019-04-01 00:00:00

  • Protective effects of lentinan on lipopolysaccharide induced inflammatory response in intestine of juvenile taimen (Hucho taimen, Pallas).

    abstract::Antioxidant effects of lentinan on LPS induced inflammatory response in intestine of juvenile taimen were evaluated, and its prebiotic-like efficacy on intestinal microbiota was also investigated. The results showed that LPS decreased the activities of antioxidant enzymes and increased the expression levels of inflamm...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.09.121

    authors: Ren G,Xu L,Lu T,Zhang Y,Wang Y,Yin J

    更新日期:2019-01-01 00:00:00

  • Study of the migration and molecular structure of starch and protein in rice kernel during heating.

    abstract::Leaching and re-absorption of leached solids and changes in kernel structure are of prime importance for the quality of cooked rice. This paper describes the contents and structural changes of both starch and protein in leached solids and rice kernels. Under actual cooking conditions, the leached solids increased with...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.10.080

    authors: Zhu L,Bi S,Wu G,Gong B,Zhang H,Wang L,Qian H,Qi X

    更新日期:2020-03-15 00:00:00

  • Optimized production and characterization of a detergent-stable protease from Lysinibacillus fusiformis C250R.

    abstract::In this study, we aimed to optimize the cultural and nutritional conditions for protease production by Lysinibacillus fusiformis strain C250R in submerged fermentation process using statistical methodology. The most significant factors (gruel, wheat bran, yeast extract, and FeSO4) were identified by Plackett-Burman de...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.03.051

    authors: Mechri S,Kriaa M,Ben Elhoul Berrouina M,Omrane Benmrad M,Zaraî Jaouadi N,Rekik H,Bouacem K,Bouanane-Darenfed A,Chebbi A,Sayadi S,Chamkha M,Bejar S,Jaouadi B

    更新日期:2017-08-01 00:00:00

  • The C-terminal α-helices of mammalian Hsc70 play a critical role in the stabilization of α-synuclein binding and inhibition of aggregation.

    abstract::Protein misfolding, followed by aggregation and amyloid formation is an underlying pathological hallmark in a number of prevalent diseases, including Parkinson's (PD), Alzheimer's (AD) and Type 2 diabetes (T2D). In the case of PD, the aggregation of α-synuclein protein (α-syn) has been shown to be highly cytotoxic and...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.10.089

    authors: Chaari A,Eliezer D,Ladjimi M

    更新日期:2016-02-01 00:00:00

  • Binding of bivalent cations by xanthan in aqueous solution.

    abstract::The interaction between xanthan and selected bivalent cations (Ca2+, Mg2+, Mn2+, Fe2+, Cu2+, Zn2+, Cd2+ and Pb2+) was studied by means of conductometry, viscometry, and nuclear magnetic resonance spectroscopy. Xanthan from Xanthomonas campestris was studied in comparison with dextran from Leuconostoc mesenteroides. Wh...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2008.06.001

    authors: Bergmann D,Furth G,Mayer C

    更新日期:2008-10-01 00:00:00

  • Levan promotes antiproliferative and pro-apoptotic effects in MCF-7 breast cancer cells mediated by oxidative stress.

    abstract::Exopolysaccharides are high-valued bio-products produced by various microbial species and have been described to possess biological response modifying activities. These bio-products have been effective as therapeutic agents in various human disease conditions. The objective of this study was to examine the effects of ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.04.035

    authors: Queiroz EAIF,Fortes ZB,da Cunha MAA,Sarilmiser HK,Barbosa Dekker AM,Öner ET,Dekker RFH,Khaper N

    更新日期:2017-09-01 00:00:00

  • Ginger polysaccharides induced cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells.

    abstract::In this study, ginger polysaccharide (GP) was obtained from ginger by enzymatic method, its chemical properties and antitumor activity were investigated. The results indicated that the composition and proportion of GP were l‑rhamnose, d‑arabinose, d‑mannose, d‑glucose and d‑galactose in a molar ratio of 3.64:5.37:3.04...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.10.169

    authors: Wang Y,Wang S,Song R,Cai J,Xu J,Tang X,Li N

    更新日期:2019-02-15 00:00:00

  • Exopolysaccharides production by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12: Optimization of fermentation variables and characterization of structure and bioactivities.

    abstract::In this study, the most important variables (incubation temperature, fermentation time and yeast extract concentration) responsible for the exopolysaccharides (EPSs) production by Lactobacillus acidophilus LA5 and Bifidobacterium animalis subsp. lactis BB12 were screened. The EPSs synthesize by LA5, BB12, and their co...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.11.084

    authors: Amiri S,Rezaei Mokarram R,Sowti Khiabani M,Rezazadeh Bari M,Alizadeh Khaledabad M

    更新日期:2019-02-15 00:00:00

  • Chitin from Antarctic krill shell: Eco-preparation, detection, and characterization.

    abstract::Antarctic krill is a nutrient-rich crustacean that is one of the main species in the Antarctic ecosystem. Antarctic krill shell (AKS) can be used as raw materials to prepare chitin. In this study, lactic acid and dispase were used to prepare Antarctic krill chitin (AKC-1). Amino-monosaccharide contents of chitin sampl...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.08.244

    authors: Yu Y,Liu X,Miao J,Leng K

    更新日期:2020-12-01 00:00:00

  • A novel cold-adapted and glucose-tolerant GH1 β-glucosidase from Exiguobacterium antarcticum B7.

    abstract::A novel GH1 β-glucosidase (EaBgl1A) from a bacterium isolated from Antarctica soil samples was recombinantly overexpressed in Escherichia coli cells and characterized. The enzyme showed unusual pH dependence with maximum activity at neutral pH and retention of high catalytic activity in the pH range 6 to 9, indicating...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.09.018

    authors: Crespim E,Zanphorlin LM,de Souza FH,Diogo JA,Gazolla AC,Machado CB,Figueiredo F,Sousa AS,Nóbrega F,Pellizari VH,Murakami MT,Ruller R

    更新日期:2016-01-01 00:00:00

  • Evaluations of physicochemical and biological properties of pullulan-based films incorporated with cinnamon essential oil and Tween 80.

    abstract::The effects of cinnamon essential oil (CEO) and Tween 80 addition on the structural, physical, antioxidant and antimicrobial properties of the pullulan-based edible films were investigated in this study. The results demonstrated that the incorporation of CEO in pullulan-based composite films decreased their tensile st...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.10.194

    authors: Chu Y,Xu T,Gao C,Liu X,Zhang N,Feng X,Liu X,Shen X,Tang X

    更新日期:2019-02-01 00:00:00

  • Preparation of fish gelatin and fish gelatin/poly(L-lactide) nanofibers by electrospinning.

    abstract::Ultrafine fibers were successfully fabricated from Channel catfish skin-extracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofiber...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2010.06.002

    authors: An K,Liu H,Guo S,Kumar DN,Wang Q

    更新日期:2010-10-01 00:00:00

  • Optimized antioxidant peptides fractions preparation and secondary structure analysis by MIR.

    abstract::The soybean protein was hydrolyzed by Alcalase Food grade (FG) 2.4 L and the optimal hydrolysis parameters of strongest antioxidant capacity of peptides were obtained using response surface methodology (RSM). The effects of reaction temperature, pH value and ratio of enzyme/soybean protein powder ([E/S]) on the 2,2-di...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2013.04.008

    authors: Lin S,Wang J,Zhao P,Pang Y,Ye H,Yuan Y,Liu J,Jones G

    更新日期:2013-08-01 00:00:00

  • Enhancing chimeric hydrophobin II-vascular endothelial growth factor A165 expression in Pichia pastoris and its efficient purification using hydrophobin counterpart.

    abstract::We report cloning and expressing of recombinant human VEGF-A165, fused at the N-terminal with Hydrophobin II (HFBII) from Trichoderma reseei, in yeast Pichia pastoris. We validated the construct using SDS-PAGE and ELISA against VEGF-A165 and efficiently performed protein purification and enrichment based on HFBII coun...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.08.080

    authors: Arjmand S,Tavasoli Z,Ranaei Siadat SO,Saeidi B,Tavana H

    更新日期:2019-10-15 00:00:00

  • Stimuli-responsive self-assembly of cellulose nanocrystals (CNCs): Structures, functions, and biomedical applications.

    abstract::Cellulose nanocrystals (CNCs) have received a significant amount of attention from the researchers. It is used as a nanomaterial for various applications due to its excellent physiochemical properties for the last few decades. Self-assembly is a phenomenon where autonomous reorganization of randomly oriented species o...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2020.03.171

    authors: Ganguly K,Patel DK,Dutta SD,Shin WC,Lim KT

    更新日期:2020-07-15 00:00:00

  • Evaluation of the mucoadhesive properties of chitosan nanoparticles prepared using different chitosan to tripolyphosphate (CS:TPP) ratios.

    abstract::Mucoadhesive molecules such as chitosan, can allow targeting of a particular tissue to prolong residence time and subsequently improve bioavailability. The purpose of this study was to investigate chitosan-tripolyphosphate (CS:TPP) nanoparticles and to evaluate the interaction between nanoparticles of different CS:TPP...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.09.185

    authors: Hejjaji EMA,Smith AM,Morris GA

    更新日期:2018-12-01 00:00:00

  • Probing the binding effects of zinc and cadmium with garlic phytocystatin: Implication of the abiotic stress on garlic phytocystatin.

    abstract::Abiotic stress induced by heavy metals retards the growth and development of plants. Therefore, it is essential to have an insight into the potential toxic effects of heavy metals. The present article investigates the effect of zinc and cadmium on the structure and function of garlic phytocystatin (GPhyCys). The cyste...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.04.036

    authors: Siddiqui MF,Bano B

    更新日期:2019-07-15 00:00:00

  • Influences of drying methods on the structural, physicochemical and antioxidant properties of exopolysaccharide from Lactobacillus helveticus MB2-1.

    abstract::In this study, in order to evaluate influences of different drying methods on the structural characteristics, physicochemical properties and antioxidant activities of exopolysaccharides (EPS) from Lactobacillus helveticus MB2-1, three drying methods, including spray-drying (SD), freeze-drying (FD) and spray freeze-dry...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.04.196

    authors: Xiao L,Li Y,Tian J,Zhou J,Xu Q,Feng L,Rui X,Fan X,Zhang Q,Chen X,Dong M,Li W

    更新日期:2020-08-15 00:00:00

  • A thermophilic and thermostable xylanase from Caldicoprobacter algeriensis: Recombinant expression, characterization and application in paper biobleaching.

    abstract::A novel xylanase gene xynBCA, encoding a polypeptide of 439 residues (XynBCA), was cloned from Caldicoprobacter algeriensis genome and recombinantly expressed in Escherichia coli BL21(DE3). The amino acid sequence analysis showed that XynBCA belongs to the glycoside hydrolase family 10. The purified recombinant enzyme...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.162

    authors: Mhiri S,Bouanane-Darenfed A,Jemli S,Neifar S,Ameri R,Mezghani M,Bouacem K,Jaouadi B,Bejar S

    更新日期:2020-12-01 00:00:00

  • Isolation and further structural characterization of lignins from the valonea of Quercus variabilis.

    abstract::The isolation process of alkali lignin (AL) from the valonea of Quercus variabilis Blume was optimized (liquid/solid ratio, 12.21; isolation time, 4.21h; isolation temperature, 42.21°C; and alkali concentration, 0.85mol/L) using the response surface method (RSM), with the highest isolation rate obtained being 22.67%. ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.01.008

    authors: Yang L,Wang D,Zhou D,Zhang Y,Yang T

    更新日期:2017-04-01 00:00:00

  • Characterization and antioxidant activities of polysaccharides from the leaves of Lilium lancifolium Thunb.

    abstract::In this study, LLP-1, LLP-2, and LLP-3 three novel polysaccharide fractions were purified from the leaves of Lilium lancifolium, and their physicochemical characterizations and antioxidant properties were investigated by chemical methods, high performance gel permeation chromatography (HPGPC), high performance liquid ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.07.028

    authors: Xu Z,Wang H,Wang B,Fu L,Yuan M,Liu J,Zhou L,Ding C

    更新日期:2016-11-01 00:00:00

  • Silk fibroin as a non-thrombogenic biomaterial.

    abstract::Silk fibroin (SF), is a very attractive protein-polymer, being processed into a variety of formats to match structural and morphological features for specific biomedical applications. The aim of the present work is to investigate blood compatibility of two forms, films and scaffolds, of silk fibroin-N,N' methylene bis...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2016.01.088

    authors: Adalı T,Uncu M

    更新日期:2016-09-01 00:00:00

  • Hydrophobic-modified montmorillonite coating onto crosslinked chitosan as the core-shell micro-sorbent for iodide adsorptive removal via Pickering emulsion polymerization.

    abstract::The radioactive iodine from nuclear wastes caused severe environmental contamination and public health concern. Chitosan and montmorillonite as green materials of the favorable adsorptivity were proposed for iodine treatment. Rather than intercalation, the crosslinked chitosan proposedly via Pickering emulsion polymer...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.09.065

    authors: Li Q,Mao Q,Yang C,Zhang S,He G,Zhang X,Zhang W

    更新日期:2019-12-01 00:00:00

  • A polysaccharide from Hedyotis diffusa interrupts metastatic potential of lung adenocarcinoma A549 cells by inhibiting EMT via EGFR/Akt/ERK signaling pathways.

    abstract::In this study we investigated the potential effects of a polysaccharide (HDP) from Hedyotis diffusa on the metastasis in human lung adenocarcinoma A549 cells. HDP (25, 50 and 100μg/ml) significantly suppressed the cell adhesion, invasion and migration of A549 cells in a dose dependent manner by downregulation of matri...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.02.040

    authors: Lin L,Cheng K,He Z,Lin Q,Huang Y,Chen C,Xie Z,Chen L,Liang Z

    更新日期:2019-05-15 00:00:00

  • Purification, characterization, antioxidant and anticancer activities of novel polysaccharides extracted from Bachu mushroom.

    abstract::Two novel polysaccharide fractions (HLP1-1 and HLP2-1) were purified from crude polysaccharides of Helvella leucopus by using DEAE-52 column (2.6cm×20cm) and Sephadex G-150 column (1.6×60cm). The characterization, antioxidant and anticancer activities of HLP1-1 and HLP2-1 were investigated. The GPC results showed that...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.09.088

    authors: Zeng D,Zhu S

    更新日期:2018-02-01 00:00:00

  • Functional properties of chitosan films modified by snail mucus extract.

    abstract::Snail mucus is an attractive natural substance, which is increasingly used in cosmetic creams and syrups thanks to its emollient, moisturizing, protective and reparative properties. The aim of the present study was to explore the physicochemical properties of chitosan-based films added with snail mucus extracted from ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.11.230

    authors: Di Filippo MF,Panzavolta S,Albertini B,Bonvicini F,Gentilomi GA,Orlacchio R,Passerini N,Bigi A,Dolci LS

    更新日期:2020-01-15 00:00:00

  • Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus ventricosus.

    abstract::Orb-web spiders produce more than seven different protein-based silks/glues by specialized abdominal glands for different uses. Prey-wrapping silk is secreted by aciniform glands for wrapping prey and forming the inner layer of egg case, and is almost twice as tough as other silks because of high strength and extensib...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.12.090

    authors: Wen R,Wang K,Liu X,Li X,Mi J,Meng Q

    更新日期:2018-10-01 00:00:00