当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Purification, physico-chemical characterization and thermodynamics of chitooligosaccharide binding to cucumber (Cucumis sativus) phloem lectin.

Purification, physico-chemical characterization and thermodynamics of chitooligosaccharide binding to cucumber (Cucumis sativus) phloem lectin.

Abstract：

:A chitooligosaccharide-specific lectin has been purified from the phloem exudate of cucumber (Cucumis sativus) by affinity chromatography on chitin. The molecular weight of the cucumber phloem lectin (CPL) was determined as 51912.8Da by mass spectrometry whereas SDS-PAGE yielded a single band with a subunit mass of 26kDa, indicating that the protein is a homodimer. Peptide mass fingerprinting studies strongly suggest that CPL is identical to the 26kDa phloem protein 2 (PP2) from cucumber. CD spectroscopy indicated that CPL is a predominantly β-sheets protein. Hemagglutination activity of CPL was mostly unaffected between 4 and 90°C and between pH 4.0 and 10.0, indicating functional stability of the protein. Isothermal titration calorimetric studies indicate that the CPL dimer binds to two chitooligosaccharide ((GlcNAc)2-6) molecules with association constants ranging from 1.0×103 to 17.5×105M-1. The binding reaction was strongly enthalpy driven (ΔHb=-ve) with negative contribution from binding entropy (ΔSb=-ve). The enthalpy-driven nature of binding reactions suggests that hydrogen bonding and van der Waals interactions stabilize the CPL-chitooligosaccharide association. Enthalpy-entropy compensation was observed for the CPL-chitooligosaccharide interaction, indicating that water molecules play an important role in the binding process.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Nareddy PK,Bobbili KB,Swamy MJ

doi

10.1016/j.ijbiomac.2016.10.078

subject

Has Abstract

pub_date

2017-02-01 00:00:00

pages

910-919

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(16)31625-7

journal_volume

95

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Transcriptomic analysis of differentially expressed genes and related pathways in Harmonia axyridis after sulfoxaflor exposure.

    abstract：:Harmonia axyridis (Pallas) is a major biological control agent that is indirectly threatened by sulfoxaflor insecticide applications targeting pests in agro-ecosystems. However, the genome-wide response of H. axyridis to sulfoxaflor remains largely unknown. Here, we used high-throughput RNA-seq technology to character...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.032

    authors： Nawaz M,Hafeez M,Mabubu JI,Dawar FU,Li X,Khan MM,Hua H,Cai W

    更新日期：2018-11-01 00:00:00

  • Antimicrobial cerium ion-chitosan crosslinked alginate biopolymer films: A novel and potential wound dressing.

    abstract：:Wound dressings require good antiseptic properties, mechanical strength and, more trustably, natural material ingredients. Antimicrobial properties of cerium ions and chitosan are known and alginate based wound dressings are commercially available. In this study, the advantages of these materials were combined and alg...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.07.144

    authors： Kaygusuz H,Torlak E,Akın-Evingür G,Özen İ,von Klitzing R,Erim FB

    更新日期：2017-12-01 00:00:00

  • Vitamin B12 offers neuronal cell protection by inhibiting Aβ-42 amyloid fibrillation.

    abstract：:Protein misfolding and aggregation has been implicated as the cause of more than 20 diseases in humans such as Alzheimer's and Parkinson's and systemic amyloidosis. Retardation of Aβ- 42 aggregation is considered as a promising and challenging strategy for developing effective therapeutics against Alzheimer's disease....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.03.001

    authors： Alam P,Siddiqi MK,Chaturvedi SK,Zaman M,Khan RH

    更新日期：2017-06-01 00:00:00

  • Chitin from Antarctic krill shell: Eco-preparation, detection, and characterization.

    abstract：:Antarctic krill is a nutrient-rich crustacean that is one of the main species in the Antarctic ecosystem. Antarctic krill shell (AKS) can be used as raw materials to prepare chitin. In this study, lactic acid and dispase were used to prepare Antarctic krill chitin (AKC-1). Amino-monosaccharide contents of chitin sampl...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.08.244

    authors： Yu Y,Liu X,Miao J,Leng K

    更新日期：2020-12-01 00:00:00

  • Elucidation of cellular uptake and intracellular trafficking of heparosan polysaccharide-based micelles in various cancer cells.

    abstract：:Heparosan polysaccharide, known as a heparin precursor, can be used in drug delivery systems due to its good biocompatibility and anti-cancer effect. But few studies on the cellular uptake mechanism of heparosan polysaccharide-based nanocarrier have been investigated. Therefore, the intracellular trafficking and the u...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.133

    authors： Qiu L,Shan X,Long M,Ahmed KS,Zhao L,Mao J,Zhang H,Sun C,You C,Lv G,Chen J

    更新日期：2019-06-01 00:00:00

  • Can green solvents be alternatives for thermal stabilization of collagen?

    abstract：:"Go Green" campaign is gaining light for various industrial applications where water consumption needs to be reduced. To resolve this, industries have adopted usage of green, organic solvents, as an alternative to water. For leather making, tanning industry consumes gallons of water. Therefore, for adopting green solv...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.05.021

    authors： Mehta A,Rao JR,Fathima NN

    更新日期：2014-08-01 00:00:00

  • Self-assembly behavior of the keratose proteins extracted from oxidized Ovis aries wool fibers.

    abstract：:Water soluble keratose proteins were obtained from an Ovis Aries wool using peracetic acid oxidation. The wool samples and the extracted keratose proteins were characterized by using FTIR, XRD, SEM and TGA techniques. Fractions of α-keratose (MW = 43-53 kDa) along with protein species with molecular weights between 23...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.129

    authors： Pakkaner E,Yalçın D,Uysal B,Top A

    更新日期：2019-03-15 00:00:00

  • Preparation and characterization of pH-responsive guar gum microspheres.

    abstract：:Guar gum, being the natural polymer is renewable, nontoxic, biocompatible and biodegradable. Therefore, it is the perfect material to formulate particulates or microspheres for potential applications in pharmaceutical. The formulation of material in nano/microsphere scale offers new rich in application potential. In v...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.09.045

    authors： Sharma S,Kaur J,Sharma G,Thakur KK,Chauhan GS,Chauhan K

    更新日期：2013-11-01 00:00:00

  • Characterization of a d-tagatose 3-epimerase from Caballeronia fortuita and its application in rare sugar production.

    abstract：:Recently, rare sugars have caused extensively attention due to their beneficial physiological functions and potential applications in food systems and medical fields. Ketose 3-epimerase (KEase) can catalyze reversibly the epimerization between ketoses which is the pivotal enzyme in Izumoring strategy and an effective ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.112

    authors： Li S,Chen Z,Zhang W,Guang C,Mu W

    更新日期：2019-10-01 00:00:00

  • Immobilization and stabilization of cholesterol oxidase on modified sepharose particles.

    abstract：:For the cholesterol oxidase from Brevibacterium sp. M201008 was not stable as free enzyme form, it had been covalently immobilized onto functionalized sepharose particles that were activated with N-ethyl-N'-3-dimethylaminopropyl carbodiimide (EDC).The optimal conditions of enzyme immobilization were determined, and th...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.01.026

    authors： Chen Y,Xin Y,Yang H,Zhang L,Zhang Y,Xia X,Tong Y,Wang W

    更新日期：2013-05-01 00:00:00

  • Alkaline proteases from a newly isolated Micromonospora chaiyaphumensis S103: Characterization and application as a detergent additive and for chitin extraction from shrimp shell waste.

    abstract：:The present study was undertaken to characterize the extracellular thermostable serine alkaline proteases from newly actinomycete strain Micromonospora chaiyaphumensis S103 and to describe their evaluation in commercial detergents and shrimp waste deproteinization. This proteolytic crude extract was active and stable ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.10.036

    authors： Mhamdi S,Ktari N,Hajji S,Nasri M,Sellami Kamoun A

    更新日期：2017-01-01 00:00:00

  • Production of novel chia-mucilage nanocomposite films with starch nanocrystals; An inclusive biological and physicochemical perspective.

    abstract：:In the current study, chia mucilage composite films with starch nanocrystals (3% and 6%) were produced. The films were analyzed physicochemically (FT-IR, AFM, TGA, DSC), mechanically (Tensile strength and contact angle) and biologically (antimicrobial, antioxidant and cytotoxicity) properties. The incorporation of sta...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.146

    authors： Mujtaba M,Koc B,Salaberria AM,Ilk S,Cansaran-Duman D,Akyuz L,Cakmak YS,Kaya M,Khawar KM,Labidi J,Boufi S

    更新日期：2019-07-15 00:00:00

  • Analysis of hydrodynamic data for denatured globular proteins in terms of the wormlike cylinder model.

    abstract：:Hydrodynamic data, i.e. intrinsic viscosity and sedimentation coefficient, for denatured globular proteins in 6 M guanidinium chloride have been re-analysed in terms of the Yamakawa-Fujii theory of the wormlike cylinder model. Molecular parameters thus obtained ((mean value of R2 0/M) infinity, the Kuhn statistical se...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(91)90078-9

    authors： Bohdanecký M,Petrus V

    更新日期：1991-08-01 00:00:00

  • Chitosan production from Paecilomyces saturatus using three monosaccharides via mixture design.

    abstract：:In this study, we demonstrate that chitosan is produced from Paecilomyces saturatus fungi using ternary monosaccharide carbon sources liquid cultivation via mixture design strategy. Sixteen experiments were carried out to obtain regression equations of fungal dry mycelial biomass (W), chitosan ratio (R), and deacetyla...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.08.256

    authors： Lin CH,Wang PH,Wen TC

    更新日期：2019-12-01 00:00:00

  • Crystal structure of prephenate dehydrogenase from Streptococcus mutans.

    abstract：:Prephenate dehydrogenase (PDH) is a bacterial enzyme that catalyzes conversion of prephenate to 4-hydroxyphenylpyruvate through the oxidative decarboxylation pathway for tyrosine biosynthesis. This enzymatic pathway exists in prokaryotes but is absent in mammals, indicating that it is a potential target for the develo...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.07.009

    authors： Ku HK,Do NH,Song JS,Choi S,Yeon SH,Shin MH,Kim KJ,Park SR,Park IY,Kim SK,Lee SJ

    更新日期：2011-11-01 00:00:00

  • Development, physicochemical characterization and cytotoxicity of selenium nanoparticles stabilized by beta-lactoglobulin.

    abstract：:Novel Selenium nanoparticles (SeNPs) were developed using beta-lactoglobulin (Blg) as a stabilizer in redox systems of selenite and ascorbic acid in this study. Particle size, morphology, stability, and in vitro biological activity of synthesized Blg stabilized selenium nanoparticles (Blg-SeNPs) were characterized by ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.09.117

    authors： Zhang J,Teng Z,Yuan Y,Zeng QZ,Lou Z,Lee SH,Wang Q

    更新日期：2018-02-01 00:00:00

  • pH-sensitive sodium alginate/poly(vinyl alcohol) hydrogel beads prepared by combined Ca2+ crosslinking and freeze-thawing cycles for controlled release of diclofenac sodium.

    abstract：:In order to enhance the drug entrapment efficiency and improve the swelling behaviors of drug delivery system, Ca2+ crosslinking and freeze-thawing (FT) cycle techniques were used to prepare sodium alginate/poly(vinyl alcohol) (SA/PVA) hydrogel beads. The mixture solution of SA and PVA was firstly crosslinked with Ca2...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.03.004

    authors： Hua S,Ma H,Li X,Yang H,Wang A

    更新日期：2010-06-01 00:00:00

  • Biochemical and biophysical characterization of a mycoredoxin protein glutaredoxin A1 from Corynebacterium pseudotuberculosis.

    abstract：:Glutaredoxin A1 from Corynebacterium pseudotuberculosis was shown to be a mycoredoxin protein. In this study, we established a process to overexpress and purify glutaredoxin A1. The aim of this study was the investigation of the Glutaredoxin A1 from C. pseudotuberculosis behavior under different redox environments and...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.10.063

    authors： Eberle RJ,Kawai LA,de Moraes FR,Tasic L,Arni RK,Coronado MA

    更新日期：2018-02-01 00:00:00

  • Interpenetrating polymer network (IPN) hydrogel microspheres for oral controlled release application.

    abstract：:Interpenetrating polymer network (IPN) hydrogel microspheres of sodium carboxymethyl cellulose (NaCMC) and poly(vinyl alcohol) (PVA) were prepared by water-in-oil (w/o) emulsion crosslinking method for oral controlled release delivery of a non-steroidal anti-inflammatory drug, diclofenac sodium (DS). The microspheres ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2011.10.020

    authors： Banerjee S,Siddiqui L,Bhattacharya SS,Kaity S,Ghosh A,Chattopadhyay P,Pandey A,Singh L

    更新日期：2012-01-01 00:00:00

  • Dendrobium officinale polysaccharides attenuate learning and memory disabilities via anti-oxidant and anti-inflammatory actions.

    abstract：:The aim of this study was to explore the therapeutic effect and underling mechanism of Dendrobium officinale polysaccharides (DOPS) on two well-established animal models of learning and memory disabilities. Model of estrogen deficiency caused learning and memory disability can be induced by ovariectomy in mice, and mi...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.12.230

    authors： Liang J,Wu Y,Yuan H,Yang Y,Xiong Q,Liang C,Li Z,Li C,Zhang G,Lai X,Hu Y,Hou S

    更新日期：2019-04-01 00:00:00

  • Role of 13-(di)phenylalkyl berberine derivatives in the modulation of the activity of human topoisomerase IB.

    abstract：:Topoisomerases IB are anticancer and antimicrobial targets whose inhibition by several natural and non-natural compounds has been documented. The inhibition effect by berberine and some 13-(di)phenylalkyl berberine derivatives has been tested towards human topoisomerase IB. Derivatives belonging to the 13-diphenylalky...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.02.051

    authors： Vieira S,Castelli S,Falconi M,Takarada J,Fiorillo G,Buzzetti F,Lombardi P,Desideri A

    更新日期：2015-01-01 00:00:00

  • Structural analysis of homologous repeated domains in alpha-actinin and spectrin.

    abstract：:The amino acid sequences of chick and slime mould alpha-actinin each contain four repeats of approximately 122 residues. These repeats are homologous to the 18-22 repeats, each of approximately 106 residues, found in the alpha and beta subunits of spectrin and fodrin, and to the multiple repeats of approximately 110 r...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(89)90047-0

    authors： Davison MD,Baron MD,Critchley DR,Wootton JC

    更新日期：1989-04-01 00:00:00

  • Study of the complex coacervation mechanism between the lysing enzyme from T. harzianum and polyallylamine hydrochloride.

    abstract：:Complex coacervation was achieved by mixing the lysing enzyme from T. harzianum (LYS) with polyallylamine hydrochloride (PAH). We show in this work that the study electrostatic complexes conformation can lead to the formation of dense complexes. We systematically investigated the effects of pH and the mass ratio on th...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.11.266

    authors： Bey H,Gtari W,Aschi A

    更新日期：2019-03-01 00:00:00

  • Development of a human epidermal growth factor derivative with EGFR-blocking and depleted biological activities: A comparative in vitro study using EGFR-positive breast cancer cells.

    abstract：:Epidermal growth factor (EGF) is a local growth factor that stimulates cell growth, proliferation, and differentiation by binding to its receptor EGFR. EGF and EGFR are involved in many aspects of the development of carcinomas. Because EGFR has been found to be over-expressed in many tumors of epithelial origin, it is...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.05.035

    authors： Mehrabi M,Mansouri K,Soleymani B,Hoseinkhani Z,Shahlaie M,Khodarahmi R

    更新日期：2017-10-01 00:00:00

  • Molecular structures, chemical properties and biological activities of polysaccharide from Smilax glabra rhizome.

    abstract：:The water-soluble crude polysaccharides, extracted from the rhizome of Smilax glabra, were fractionated using an anion exchange chromatography, yielding two fractions, F1 and F2. The crude and fractions (F1 and F2) mainly consisted of carbohydrates (66.7%-91.1%), proteins (7.30%-23.9%) and minor amount of sulfates (1....

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.138

    authors： Lee J,Li C,Surayot U,Yelithao K,Lee S,Park W,Tabarsa M,You S

    更新日期：2018-12-01 00:00:00

  • Inhibition of tau aggregation and associated cytotoxicity on neuron-like cells by calycosin.

    abstract：:In this study, the in vitro assembly of tau and anti-amyloidogenic properties of one naturally occurring phytoestrogen, calycosin, was investigated by spectroscopic techniques including ThT and ANS fluorescence, CD, Congo red absorbance as well as TEM analysis. Afterwards the cytotoxicity of different amyloid species ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.12.030

    authors： Zhenxia Z,Min L,Peikui Y,Zikai C,Yaqun L,Junli W,Fenlian Y,Yuzhong Z

    更新日期：2020-12-07 00:00:00

  • Molecular mechanism on cadmium-induced activity changes of catalase and superoxide dismutase.

    abstract：:Cadmium contributes to adverse effects of organisms probably because of its ability to induce oxidative stress via alterations in activities of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD), but their molecular mechanisms remain unclear. We investigated the molecular mechanism of CAT and SOD respon...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.02.037

    authors： Wang J,Zhang H,Zhang T,Zhang R,Liu R,Chen Y

    更新日期：2015-01-01 00:00:00

  • Chitosan‑zinc sulfide nanoparticles, characterization and their photocatalytic degradation efficiency for azo dyes.

    abstract：:Herein, chitosan‑zinc sulfide nanoparticles (CS-ZnS-NPs) were developed as an efficient photocatalyst for the degradation of toxic dyes. The as-synthesized CS-ZnS-NPs were analyzed using XRD, FTIR, SEM, and EDS. The functional groups of CS-ZnS-NPs were validated with FTIR spectroscopy. The SEM envisaged the average pa...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.02.310

    authors： Aziz A,Ali N,Khan A,Bilal M,Malik S,Ali N,Khan H

    更新日期：2020-06-15 00:00:00

  • A biotechnological approach to immunotherapy: Antivenom against Crotalus durissus cascavella snake venom produced from biodegradable nanoparticles.

    abstract：:Snakebite envenoming is a tropical disease neglected worldwide. In Brazil, the Crotalus durissus cascavella (CDC) snake belongs to a genus with venom of highest lethality. A search for new immunoadjuvants aimed to expand the therapeutic alternatives to improve vaccines and antivenom. This approach proposed to produce ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.09.203

    authors： Gláucia-Silva F,Torres-Rêgo M,Rocha Soares KS,Damasceno IZ,Tambourgi DV,Silva-Júnior AAD,Fernandes-Pedrosa MF

    更新日期：2018-12-01 00:00:00

  • Tantalizing role of p53 molecular pathways and its coherent medications in neurodegenerative diseases.

    abstract：:Neurodegenerative diseases are incongruous, commonly age-related disorders characterized by progressive neuronal loss, comprising the most prevalent being Alzheimer's disease, Parkinson's disease, and Huntington's disease. Perilous health states are anticipated following the neurodegeneration. Their etiology remains l...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2021.01.042

    authors： Talebi M,Talebi M,Kakouri E,Farkhondeh T,Pourbagher-Shahri AM,Tarantilis PA,Samarghandian S

    更新日期：2021-01-10 00:00:00