A biotechnological approach to immunotherapy: Antivenom against Crotalus durissus cascavella snake venom produced from biodegradable nanoparticles.

Abstract:

:Snakebite envenoming is a tropical disease neglected worldwide. In Brazil, the Crotalus durissus cascavella (CDC) snake belongs to a genus with venom of highest lethality. A search for new immunoadjuvants aimed to expand the therapeutic alternatives to improve vaccines and antivenom. This approach proposed to produce small and narrow-sized cationic CDC venom-loaded chitosan nanoparticles (CHNP) able to induce antibody response against the CDC venom. The ionic gelation method induced the formation of stable and slightly smooth spherical nanoparticles (<160 nm) with protein loading efficiency superior to 90%. The interactions between venom proteins and CHNP assessed using FT-IR spectroscopy corroborated with the in vitro release behavior of proteins from nanoparticles. Finally, the immunization animal model using BALB/c mice demonstrated the higher effectiveness of CDC venom-loaded CHNP compared to aluminum hydroxide, a conventional immunoadjuvant. Thus, CHNPs loaded with CDC venom exhibited a promising biotechnological approach to immunotherapy.

journal_name

Int J Biol Macromol

authors

Gláucia-Silva F,Torres-Rêgo M,Rocha Soares KS,Damasceno IZ,Tambourgi DV,Silva-Júnior AAD,Fernandes-Pedrosa MF

doi

10.1016/j.ijbiomac.2018.09.203

subject

Has Abstract

pub_date

2018-12-01 00:00:00

pages

1917-1924

issue

Pt B

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(18)33607-9

journal_volume

120

pub_type

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