Abstract:
:Water soluble keratose proteins were obtained from an Ovis Aries wool using peracetic acid oxidation. The wool samples and the extracted keratose proteins were characterized by using FTIR, XRD, SEM and TGA techniques. Fractions of α-keratose (MW = 43-53 kDa) along with protein species with molecular weights between 23 kDa and 33 kDa were identified in the SDS-PAGE analysis result of the extracted protein mixture. DLS and AFM experiments indicated that self-assembled globular nanoparticles with diameters between 15 nm and 100 nm formed at 5 mg/ml keratose concentration. On the other hand, upon incubation of 10 w % keratose solutions at 37 °C and 50 °C, interconnected keratose hydrogels with respective storage modulus (G') values of 0.17 ± 0.03 kPa and 3.7 ± 0.5 kPa were obtained. It was shown that the keratose hydrogel prepared at 37 °C supported L929 mouse fibroblast cell proliferation which suggested that these keratose hydrogels could be promising candidates in soft tissue engineering applications.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Pakkaner E,Yalçın D,Uysal B,Top Adoi
10.1016/j.ijbiomac.2018.12.129subject
Has Abstractpub_date
2019-03-15 00:00:00pages
1008-1015eissn
0141-8130issn
1879-0003pii
S0141-8130(18)35094-3journal_volume
125pub_type
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