当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Characterization of a novel bifunctional mannuronan C-5 epimerase and alginate lyase from Pseudomonas mendocina. sp. DICP-70.

Characterization of a novel bifunctional mannuronan C-5 epimerase and alginate lyase from Pseudomonas mendocina. sp. DICP-70.

Abstract：

:Alginate is a family of industrially important linear polymers consisting of β-D-mannuronic acid (M) and its C-5 epimer α-L-guluronic acid (G). The function of alginate is closely related to the ratio of M/G. Mannuronan C-5 epimerase, which converts M to G, is a key enzyme involved in the biosynthesis of alginate. A new mannuronan C-5 epimerase isolated from Pseudomonas mendocina. sp. DICP-70 named PmC5A was characterized in this study. From the 1H NMR analysis of the products, we have found that PmC5A possesses alginate lyase function in addition to mannuronan C-5-epimerase. The optimal pH and temperature of lyase and epimerase were found to be 8.0, 9.0 and 40 °C, 30 °C, respectively. PmC5A also shows lyase activity toward PolyMG and G-blocks.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Sun M,Sun C,Li T,Li K,Yan S,Yin H

doi

10.1016/j.ijbiomac.2020.02.126

subject

Has Abstract

pub_date

2020-05-01 00:00:00

pages

662-670

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(19)38952-4

journal_volume

150

pub_type

杂志文章

相关文献

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES文献大全
  • Exploration of DNA binding mode, chemical nuclease, cytotoxic and apoptotic potentials of diketone based oxovanadium(IV) complexes.

    abstract：:Two diketone based oxovanadium complexes, viz., bis(4,4,4-trifluoro-1-phenylbutane-1,3-dionato)oxovanadium(IV) (1) and bis(1,1,1-trifluoropentane-2,4-dionato)oxovanadium(IV) (2), have been synthesized and characterized by spectroscopic and analytical techniques. The DNA binding and the cleaving ability of the complexe...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.02.027

    authors： Inamdar PR,Sheela A

    更新日期：2015-05-01 00:00:00

  • Cellulose extraction from methyltrioctylammonium chloride pretreated sugarcane bagasse and its application.

    abstract：:Cellulose, the most abundant feedstock of chemicals and energy is extracted from various agro-industrial wastes, such as sugarcane bagasse (SB). Pretreatment of SB with ionic liquids improves extraction of cellulose, yet the use of ionic liquid is hindered by its high cost. In this study, cellulose was extracted from ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.09.151

    authors： Ejaz U,Muhammad S,Ali FI,Hashmi IA,Sohail M

    更新日期：2020-12-15 00:00:00

  • Characterization of trimethyl chitosan/polyethylene glycol derivatized chitosan blend as an injectable and degradable antimicrobial delivery system.

    abstract：:Advanced local delivery systems are needed as adjunctive treatments for severe injuries with high infection rates, such as open fractures. Chitosan systems have been investigated as antimicrobial local delivery systems for orthopaedic infection but possess mismatches between elution and degradation properties. Derivat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.04.075

    authors： Boles LR,Bumgardner JD,Fujiwara T,Haggard WO,Guerra FD,Jennings JA

    更新日期：2019-07-15 00:00:00

  • Molecular crowding prevents the aggregation of protein-dextran conjugate by inducing structural changes, improves its functional properties, and stabilizes it in nanoemulsions.

    abstract：:In most of the recent research on emulsions related to food products containing protein-polysaccharide interactions established via the Maillard reaction have been used as emulsifiers. Key challenges in such studies include long reaction times, uncontrollable extent of reaction, and protein denaturation and aggregatio...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.09.007

    authors： Hu M,Liu G,Du X,Zhang X,Qi B,Li Y

    更新日期：2020-12-01 00:00:00

  • Statistical optimization and operational stability of Rhizomucor miehei lipase supported on magnetic chitosan/chitin nanoparticles for synthesis of pentyl valerate.

    abstract：:The chemical-catalyzed transesterification process to produce biofuels i.e. pentyl valerate (PeVa) is environmentally unfriendly, energy-intensive with tedious downstream treatment. The present work reports the use of Rhizomucor miehei lipase (RML) crosslinked onto magnetic chitosan/chitin nanoparticles (RML-CS/CH/MNP...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.04.111

    authors： Rahman INA,Attan N,Mahat NA,Jamalis J,Abdul Keyon AS,Kurniawan C,Wahab RA

    更新日期：2018-08-01 00:00:00

  • Preparation of a novel Grifola frondosa polysaccharide-chromium (III) complex and its hypoglycemic and hypolipidemic activities in high fat diet and streptozotocin-induced diabetic mice.

    abstract：:Polysaccharide from Grifola frondosa is one of the best metal-ion chelating agents because of its structural characteristics and excellent functional activities. In this study, we synthesized and characterized a novel Grifola frondosa polysaccharide-chromium (III) [GFP-Cr(III)] complex. Response surface methodology (R...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.03.042

    authors： Guo WL,Shi FF,Li L,Xu JX,Chen M,Wu L,Hong JL,Qian M,Bai WD,Liu B,Zhang YY,Ni L,Rao PF,Lv XC

    更新日期：2019-06-15 00:00:00

  • A polysaccharide from Hedyotis diffusa interrupts metastatic potential of lung adenocarcinoma A549 cells by inhibiting EMT via EGFR/Akt/ERK signaling pathways.

    abstract：:In this study we investigated the potential effects of a polysaccharide (HDP) from Hedyotis diffusa on the metastasis in human lung adenocarcinoma A549 cells. HDP (25, 50 and 100μg/ml) significantly suppressed the cell adhesion, invasion and migration of A549 cells in a dose dependent manner by downregulation of matri...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.02.040

    authors： Lin L,Cheng K,He Z,Lin Q,Huang Y,Chen C,Xie Z,Chen L,Liang Z

    更新日期：2019-05-15 00:00:00

  • Chitosan/Sterculia striata polysaccharides nanocomplex as a potential chloroquine drug release device.

    abstract：:Nanoparticles are produced by means of polyelectrolyte complexation (PEC) of oppositely charged polycationic chitosan (CH) with polyanionic polysaccharide extracted from Sterculia striata exudates (rhamnogalacturonoglycan (RG)-type polysaccharide). The nanoparticles formed with low-molar-mass CH are larger than those ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2016.03.070

    authors： Magalhães GA Jr,Moura Neto E,Sombra VG,Richter AR,Abreu CM,Feitosa JP,Paula HC,Goycoolea FM,de Paula RC

    更新日期：2016-07-01 00:00:00

  • Extractions and rheological properties of polysaccharide from okra pulp under mild conditions.

    abstract：:This study investigated the extraction of polysaccharide from okra pulp (POP), its chemical components, and rheological properties. The results showed that the optimal extraction temperature, time, and the ration of water to material were 20-30 °C, 30 mins, and 150, respectively, giving a maximum yield of 29.4%. The P...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章,评审

    doi：10.1016/j.ijbiomac.2020.01.163

    authors： Li Y,Wang X,Lv X,Wang X,Wang X,Cui J,Yan M

    更新日期：2020-04-01 00:00:00

  • Synthesis of Fe3O4@chitosan@ZIF-8 towards removal of malachite green from aqueous solution: Theoretical and experimental studies.

    abstract：:Discharges of dyes-containing wastewater from industries have created global concern. Removal of these substances from aqueous solution is very important and essential. Magnetic chitosan coated with ZIF-8 was prepared successfully and used to eliminate cationic dye malachite green in various conditions. To find the ef...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.12.067

    authors： Zadvarzi SB,Khavarpour M,Vahdat SM,Baghbanian SM,Rad AS

    更新日期：2021-01-31 00:00:00

  • Modelling of the 3-D structure of IFN-alpha-k and characterization of its surface molecular properties.

    abstract：:The 3-D structure of IFN-alpha-k (one of the alpha-interferon family) was constructed and optimized by molecular modelling starting from the X-ray structure of IFN-beta. The molecular surface of the optimized 3-D structure of IFN-alpha-k was then evaluated and characterized for its hydrophobicity/hydrophilicity. The s...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(96)01143-9

    authors： Miertus S,Tomasi J,Mazzanti G,Chiellini EE,Solaro R,Chiellini E

    更新日期：1997-04-01 00:00:00

  • Selective regulation of RANKL/RANK/OPG pathway by heparan sulfate through the binding with estrogen receptor β in MC3T3-E1 cells.

    abstract：:Heparan sulfate (HS) is a linear anionic polysaccharide with repeating sulfated disaccharide units, which has been proven with various regulatory osteogenesis effects through multi-pathway signaling, but its impacts on receptor-activator of nuclear factor kappa beta ligand/receptor-activator of nuclear factor kappa be...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.07.308

    authors： Liu Y,Xu Z,Wang Q,Jiang Y,Wang R,Chen S,Zhu J,Zhang Y,Chen J

    更新日期：2020-10-15 00:00:00

  • Chitosan hydrogel in combination with marine peptides from tilapia for burns healing.

    abstract：:The purpose of this study was to develop a promising burns dressing. Chiosan (CS) has been widely used as biomaterials, in combination with marine peptides (MPs) extracted from seawater cultured Tilapia, the newly developed material Chitosan-Marine Peptides hydrogels (CSMP) in this study showed antibacterial activity,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.01.217

    authors： Ouyang QQ,Hu Z,Lin ZP,Quan WY,Deng YF,Li SD,Li PW,Chen Y

    更新日期：2018-06-01 00:00:00

  • Fabrication of letrozole formulation using chitosan nanoparticles through ionic gelation method.

    abstract：:In this study, the anticancer drug letrozole (LTZ) was formulated using chitosan nanoparticles (CS-NPs) with the crosslinking agent sodium tripolyphosphate (TPP). The nano-formulation was optimized by varying the concentration of drug. The prepared particles were characterized using FTIR, TGA, XRD, SEM, TEM and DLS. F...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.01.147

    authors： Gomathi T,Sudha PN,Florence JAK,Venkatesan J,Anil S

    更新日期：2017-11-01 00:00:00

  • Ganoderma atrum polysaccharide ameliorates anoxia/reoxygenation-mediated oxidative stress and apoptosis in human umbilical vein endothelial cells.

    abstract：:Ganoderma atrum polysaccharide (PSG-1), a main polysaccharide from Ganoderma atrum, possesses potent antioxidant capacity and cardiovascular benefits. The aim of this study was to investigate the role of PSG-1 in oxidative stress and apoptosis in human umbilical vein endothelial cells (HUVECs) under anoxia/reoxygenati...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.01.071

    authors： Zhang YS,Li WJ,Zhang XY,Yan YX,Nie SP,Gong DM,Tang XF,He M,Xie MY

    更新日期：2017-05-01 00:00:00

  • Molecular cloning of a small heat shock protein (sHSPII) from the cattle tick Rhipicephalus (Boophilus) annulatus salivary gland.

    abstract：:Immunoscreening of a cDNA expression library of the Rhipicephalus (Boophilus) annulatus tick with purified rabbit anti-R annulatus salivary glands antigens polyclonal antibodies led to the identification of a 661bp sequence. The sequence includes an open reading frame of 543bp encoding a protein of 180 amino acids wit...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2010.08.006

    authors： Shahein YE,El-Rahim MT,Hussein NA,Hamed RR,El-Hakim AE,Barakat MM

    更新日期：2010-12-01 00:00:00

  • Stress response and silencing verification of heat shock proteins in Dermatophagoides farinae under temperature stress.

    abstract：:Dermatophagoides farinae is a major exogenous allergen. Its ability to tolerate adverse external temperatures makes it responsible for widespread occurrence of allergies. Heat shock protein (HSP), a recognized temperature stress response gene, but its role in D. farinae remained unclear. Here, we performed a comprehen...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.12.014

    authors： Niu D,Zhao Y,Gong X,Yang R,Hu L,Zhang W

    更新日期：2020-02-01 00:00:00

  • Probing the stability of native and activated forms of alpha2-macroglobulin.

    abstract：:alpha2-Macroglobulin (alpha2M) is a 718 kDa homotetrameric proteinase inhibitor which undergoes a large conformational change upon activation. This conformational change can occur either by proteolytic attack on an approximately 40 amino acid stretch, the bait region, which results in the rupture of the four thioester...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2007.09.019

    authors： Kaczowka SJ,Madding LS,Epting KL,Kelly RM,Cianciolo GJ,Pizzo SV

    更新日期：2008-01-01 00:00:00

  • Naja kaouthia venom protein, Nk-CRISP, upregulates inflammatory gene expression in human macrophages.

    abstract：:Cysteine-Rich Secretory Proteins (CRISP) are widespread in snake venoms and known to target ion channels. More recently, CRISPs have been shown to mediate inflammatory responses. Involvement of potential receptor in CRISP-induced inflammatory reactions, however, remains unknown. A CRISP protein named as Nk-CRISP, was ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.05.169

    authors： Deka A,Sharma M,Mukhopadhyay R,Devi A,Doley R

    更新日期：2020-10-01 00:00:00

  • Extracellular degradation of medium chain length poly(beta-hydroxyalkanoates) by Comamonas sp.

    abstract：:The PHA-degrading isolate, strain P37C, was enriched from residential compost for its ability to hydrolyze the medium chain length PHA, poly(beta-hydroxyoctanoate) (PHO). It was subsequently found to grow on a wide range of PHAs, including both short chain length and medium chain length PHAs. The isolate was identifie...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/s0141-8130(99)00027-6

    authors： Quinteros R,Goodwin S,Lenz RW,Park WH

    更新日期：1999-06-01 00:00:00

  • Studies on structure and antioxidant properties of a heteroglycan isolated from wild edible mushroom Lentinus sajor-caju.

    abstract：:A water-soluble heteroglycan (PS-I) isolated from the aqueous extract of a wild edible mushroom Lentinus sajor-caju showed average molecular weight ∼1.79×105Da. The structure of the polysaccharide was determined using chemical and 1D/2D NMR experiments. Acid hydrolysis indicated the presence of d-glucose, d-galactose,...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.08.163

    authors： Pattanayak M,Maity P,Samanta S,Sen IK,Manna DK,Nandi AK,Ghosh S,Acharya K,Islam SS

    更新日期：2018-02-01 00:00:00

  • Lactic acid oligomer (OLLA) grafted gum arabic based green adhesive for structural applications.

    abstract：:L-Lactic acid (LA)-grafted-gum arabic (GA) graft copolymer [OLLA-g-GA] was prepared by using microwave heating in which the hydrophilic GA was grafted with insitu grown lactic acid oligomer. The grafting at OH and NH groups in GA backbone with oligo-(l-lactic acid) (OLLA) chains proved with the help of FTIR and NMR an...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2018.07.199

    authors： Tripathi N,Katiyar V

    更新日期：2018-12-01 00:00:00

  • Biodistribution profiling of the chemical modified hyaluronic acid derivatives used for oral delivery system.

    abstract：:A series of adipic acid dihydrazide (ADH)-modified hyaluronic acid (HA-ADH) compounds were synthesized and conjugated with QDots (QDots-HA conjugates) to assess the effects of the molecular weight (MW) and extent of chemical modification of HA on its biodistribution. Their physicochemical structures were confirmed by ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2013.11.027

    authors： Hsieh CM,Huang YW,Sheu MT,Ho HO

    更新日期：2014-03-01 00:00:00

  • Synthesis and conformational analysis of Aib-containing peptide modelling for N-glycosylation site in N-glycoprotein.

    abstract：:A tetrapetide containing an Aib residue, Boc-Asn-Aib-Thr-Aib-OMe, was synthesized as a peptide model for the N-glycosylation site in N-glycoproteins. Backbone conformation of the peptide and possible intramolecular interaction between the Asn and Thr side chains were elucidated by means of n.m.r. spectroscopy. Tempera...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/0141-8130(89)90003-2

    authors： Ishii H,Nadaoka O,Mimura Y,Inoue Y,Chûjô R

    更新日期：1989-12-01 00:00:00

  • Surface decoration of neohesperidin-loaded nanoliposome using chitosan and pectin for improving stability and controlled release.

    abstract：:The aim of this study was to improve the physicochemical stability of neohesperidin (NH) using nanoliposomal encapsulation in association with surface decoration strategy employing chitosan (CH) and pectin (P). Different nanoliposomal systems, i.e. NH-loaded nanoliposome (NH-NL), CH-coated NH-NL (CH-NH-NL), and P-coat...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2020.08.174

    authors： Karim N,Shishir MRI,Chen W

    更新日期：2020-12-01 00:00:00

  • Sophora subprosrate polysaccharide inhibited cytokine/chemokine secretion via suppression of histone acetylation modification and NF-κb activation in PCV2 infected swine alveolar macrophage.

    abstract：:In the present study, effect of Sophora subprosrate polysaccharide on PCV2 infection-induced inflammation and histone acetylation modification in swine alveolar macrophage 3D4/2 cells was described for the first time. The relationship between histone acetylation modifications and inflammation response was investigated...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2017.06.102

    authors： Yang J,Tan HL,Gu LY,Song ML,Wu YY,Peng JB,Lan ZB,Wei YY,Hu TJ

    更新日期：2017-11-01 00:00:00

  • Property enhancement of soy protein isolate-based films by introducing POSS.

    abstract：:To enhance the mechanical and water-resistant properties of soy protein isolate (SPI) based films, hydrophobic TriSilanolPhenyl polyhedral oligomeric silsesquioxanes (POSS) was incorporated to modify the SPI films. POSS has three SiOH groups in a molecular, which is employed to cross-link SPI with the help of 3-glycid...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2015.11.024

    authors： Xia C,Zhang S,Shi SQ,Cai L,Garcia AC,Rizvi HR,D'Souza NA

    更新日期：2016-01-01 00:00:00

  • Effects of repeated and continuous dry heat treatments on properties of sweet potato starch.

    abstract：:The influence of repeated and continuous dry heat treatments on structural, physicochemical, and digestive properties of sweet potato starch was investigated. The results showed that the original A-type crystallinity of sweet potato starch remained unchanged after dry heat treatments. The light transmittance, gelatini...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.01.225

    authors： Gou M,Wu H,Saleh ASM,Jing L,Liu Y,Zhao K,Su C,Zhang B,Jiang H,Li W

    更新日期：2019-05-15 00:00:00

  • Antitumor and antiangiogenic activity of Schisandra chinensis polysaccharide in a renal cell carcinoma model.

    abstract：:The aim of this study was to determine the antitumor and antiangiogenic effects of the Schisandra chinensis polysaccharides (SCP) in selected renal cell carcinoma (RCC) cells and evaluate its potential mechanism of action. In vitro, endothelial growth factor (VEGF) secretion by Caki-1 was blockaded in response to SCP ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2014.02.025

    authors： Qu HM,Liu SJ,Zhang CY

    更新日期：2014-05-01 00:00:00

  • Synthesis of chitosan based molecularly imprinted polymer for pipette-tip solid phase extraction of Rhodamine B from chili powder samples.

    abstract：:The purpose work is devoted to design of a simple, one-pot and green approach for the synthesis of molecularly imprinted polymer to construct a selective sorbent for pipette-tip solid phase extraction of Rhodamine B from chili powder samples and its subsequence separation and quantification by high performance liquid ...

    journal_title：International journal of biological macromolecules

    pub_type： 杂志文章

    doi：10.1016/j.ijbiomac.2019.07.196

    authors： Bagheri AR,Ghaedi M

    更新日期：2019-10-15 00:00:00