Abstract:
:Alginate is a family of industrially important linear polymers consisting of β-D-mannuronic acid (M) and its C-5 epimer α-L-guluronic acid (G). The function of alginate is closely related to the ratio of M/G. Mannuronan C-5 epimerase, which converts M to G, is a key enzyme involved in the biosynthesis of alginate. A new mannuronan C-5 epimerase isolated from Pseudomonas mendocina. sp. DICP-70 named PmC5A was characterized in this study. From the 1H NMR analysis of the products, we have found that PmC5A possesses alginate lyase function in addition to mannuronan C-5-epimerase. The optimal pH and temperature of lyase and epimerase were found to be 8.0, 9.0 and 40 °C, 30 °C, respectively. PmC5A also shows lyase activity toward PolyMG and G-blocks.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Sun M,Sun C,Li T,Li K,Yan S,Yin Hdoi
10.1016/j.ijbiomac.2020.02.126subject
Has Abstractpub_date
2020-05-01 00:00:00pages
662-670eissn
0141-8130issn
1879-0003pii
S0141-8130(19)38952-4journal_volume
150pub_type
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