Abstract:
:The conversion of α-synuclein from its natively unfolded and α-helical tetrameric forms to an amyloid conformation is central to the emergence of Parkinson's disease. Therefore, prevention of this conversion may offer an effective way of avoiding the onset of this disease or delaying its progress. At different concentrations, an aqueous extract from the edible winged kelp (Alaria esculenta), was shown to lower and to raise the melting point of α-synuclein. Size fractionation of the extract resulted in the separation of these distinct activities. The fraction below 5kDa decreased the melting point of α-synuclein, whereas the fraction above 10kDa raised the melting point. Both of these fractions were found to inhibit the formation of amyloid aggregates by α-synuclein, measured by thioflavin T dye-binding assays; this effect was further confirmed by transmission electron microscopy showing the inhibition of fibril formation. Circular dichroism analysis suggested that the incubation of α-synuclein under fibrillation conditions resulted in the loss of substantial native helical structure in the presence and absence of the fractions. It is therefore likely that the fractions inhibit fibrillation by interacting with the unfolded form of α-synuclein.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Giffin JC,Richards RC,Craft C,Jahan N,Leggiadro C,Chopin T,Szemerda M,MacKinnon SL,Ewart KVdoi
10.1016/j.neulet.2017.02.055subject
Has Abstractpub_date
2017-03-22 00:00:00pages
87-93eissn
0304-3940issn
1872-7972pii
S0304-3940(17)30171-4journal_volume
644pub_type
杂志文章abstract::This study evaluated the immediate and persistent behavioral and molecular consequences (i.e., emotional signs emerging during withdrawal and signs of neurotoxicity) following adolescent morphine exposure with a sex perspective. Basally, and prior to drug treatment, adolescent female rats showed smaller body weight an...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2019.134625
更新日期:2020-01-10 00:00:00
abstract::A particular alcohol dehydrogenase (ADH) polymorphism (allele A1) in the promoter region of the gene has been recently demonstrated to be associated with increased risk of Parkinson's disease (PD). In a case control study, we examine frequencies of ADH A1 allele in 100 PD patients (i.e. 200 alleles), 100 diseased cont...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)01770-0
更新日期:2001-06-01 00:00:00
abstract::In the present study we aimed at evaluating the prevalence and the associated clinical, neuropsychological and behavioral features of extrapyramidal symptoms in Frontotemporal Dementia (FTD) patients. Seventy-five patients fulfilling clinical criteria of FTD were consecutively enrolled. Each patient underwent clinical...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2007.05.049
更新日期:2007-07-05 00:00:00
abstract::In the brain, the stress system plays an important role in motivating continued alcohol use and relapse. The neuropeptide substance P and the neurokinin-1 receptor (NK1R) are involved in the stress response and drug reward systems. Recent findings have shown that the binding of ligands to NK1Rs decreases the self-admi...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.03.051
更新日期:2010-05-21 00:00:00
abstract::Localized moderate cooling (+10 degrees C) of the anterior and ventral part of the raphe dorsalis induced cortical synchronization and ponto-geniculo-occipital (PGO) waves followed sometimes by paradoxical sleep. Cooling the region of the raphe magnus produced cortical arousal. After depletion of brain 5-hydroxytrypta...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(76)90077-x
更新日期:1976-11-01 00:00:00
abstract::Amphiphilic monomers and dimers of acetylcholinesterase (AChE) and hydrophilic tetramers of butyrylcholinesterase (BuChE) were released by extracting human meningioma with Tris-saline and Tris-saline-Triton X-100 buffers. The amphiphilic or hydrophilic behavior of the AChE and BuChE forms was assessed by sedimentation...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)11791-g
更新日期:1995-08-04 00:00:00
abstract::The learned helplessness (LH) developed after repeat inescapable stress is a well validated animal model of human major depression and is not species specific. c-Fos, the protein product of the protooncogene c-fos, is expressed in neurons under a variety of stressors and could reflect the regional neuronal activation....
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.04.011
更新日期:2004-06-17 00:00:00
abstract::Systemic administration of nitroglycerin (NTG) consistently provokes spontaneous-like migraine attacks in migraine sufferers, and in rat it induces hyperalgesia and allodynia. In the present study we modeled migraine headache by intraperitoneal (i.p.) injection of a single dose of NTG, which produced widespread therma...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.07.026
更新日期:2016-09-06 00:00:00
abstract::The effect of dexmedetomidine, a selective alpha2-adrenoreceptor agonist and neuroprotective agent on the expression of immediate early genes and heat shock protein hsp70, was studied using quantitative in situ hybridization in a global ischemia model. At the dose previously shown to be neuroprotective dexmedetomidine...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00585-5
更新日期:1997-08-29 00:00:00
abstract::The activity of single units in the striatum of urethane-anesthetized rats was recorded before and after the systemic administration of 7-nitro-indazole (7-NI; 50 mg/kg intraperitoneally), a selective inhibitor of neuronal nitric oxide (NO) synthase. Two neuronal types were clearly distinguishable electrophysiological...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(02)00270-7
更新日期:2002-06-14 00:00:00
abstract::Metabotropic glutamate receptors (mGluR) are G-protein-coupled receptors that play a major role in modulatory pathways in the CNS and have been suggested to have pharmacological implications in pain, psychiatric disorders and other neurological states. 3-[(2-Methyl-1,3-thiazol-4-yl) ethynyl]-pyridine (MTEP) is a speci...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2005.08.032
更新日期:2006-01-02 00:00:00
abstract::Peroxisome proliferator-activated receptors (PPARs) are ligand-dependent transcription factors which belong to the nuclear receptor family. We examined whether PPARalpha agonists and resveratrol, a polyphenol contained in grapes, protect the brain against ischemia. To investigate whether resveratrol activates PPARs, w...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2003.09.001
更新日期:2003-12-11 00:00:00
abstract::Chronic stress is well known to exacerbate pain. We tested the hypothesis that neonatal handling, which induces resilience to the negative impact of stress by increasing the quality and quantity of maternal care, attenuates the mechanical hyperalgesia produced by water-avoidance stress in the adult rat. Neonatal male ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.01.066
更新日期:2015-03-30 00:00:00
abstract::Application of NMDA, or agents releasing nitric oxide (NO), onto the dendrites of hippocampal granule cells increased the levels of the mRNA encoding MAP2, a cytoskeletal component induced during periods of neurite outgrowth. Furthermore, local increases in the hybridisation signal in the molecular layer, representing...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90031-0
更新日期:1994-08-15 00:00:00
abstract::Local delivery of cytokines or other immunomodulatory components has been applied as a potential therapy for experimental autoimmune encephalomyelitis (EAE), which is used as a model of human multiple sclerosis. We have used herpes simplex virus-based vectors expressing Th2 cytokines IL-4 and IL-10 and have previously...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2004.04.059
更新日期:2004-07-08 00:00:00
abstract::Expression by rat motoneurons of mRNAs for the alpha subunit of the ciliary neurotrophic factor receptor (CNTFR alpha), receptor tyrosine kinase-C (trkC), calcitonin gene-related peptide (CGRP) and galanin was studied by in situ hybridisation, 6d after rat facial nerve transection on postnatal day 1. Similar numbers o...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(96)13124-4
更新日期:1996-11-08 00:00:00
abstract::The distribution of neurons containing the messenger RNA encoding preprotachykinin, the precursor of the neuropeptides substance P and K, was examined in the striatum of the rat. The large majority of cells densely labelled by in situ hybridization histochemistry (using a 35S-radiolabelled RNA probe complementary of t...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(89)90241-3
更新日期:1989-01-02 00:00:00
abstract::In this study, we aimed to investigate whether self-reported mild traumatic brain injury (mTBI) was associated with decreased AD-vulnerable cortical thickness, and to assess the relationship between AD-vulnerable cortical thickness and AD-related biomarker in the Alzheimer's Disease Neuroimaging Initiative subjects. W...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2017.06.055
更新日期:2017-08-10 00:00:00
abstract::The study investigates time-variant directed interactions between brain regions during the interburst-burst EEG pattern (tracé alternant) characteristic of quiet sleep in healthy neonates. The transition from interburst to burst is of particular interest as the generation of the EEG characteristics at burst onset refl...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2010.11.018
更新日期:2011-01-20 00:00:00
abstract::Evidence, based on voltage-clamp and current-clamp recording, is presented for endogenous receptors for vasoactive intestinal polypeptide (VIP) on the cell membrane of the Xenopus oocyte. At normal resting potential a hyperpolarisation is produced by VIP, associated with an increase in K+ conductance. The response is ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(87)90221-7
更新日期:1987-12-16 00:00:00
abstract::It was established that stimulation of the lateral hypothalamus (LH) can induce antinociception. Previous studies showed a role for the ventral tegmental area (VTA) and nucleus accumbens (NAc) in antinociception induced by LH stimulation through the orexinergic system. In this study, we tried to assess the involvement...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2015.05.047
更新日期:2015-07-10 00:00:00
abstract::The N-Methyl-D-Aspartate Receptors (NMDARs) are heteromeric cation channels involved in learning, memory, and synaptic plasticity, and their dysregulation leads to various neurodegenerative disorders. Recent evidence has shown that apart from the GluN1/GluN2A and GluN1/GluN2B diheteromeric ion channels, the NMDAR also...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2016.02.032
更新日期:2016-03-23 00:00:00
abstract::Day-night variations of gastrin releasing peptide (GRP) in the neuronal somal area of the SCN and the nearby region on the anterior hypothalamic area (AHA) where GRP fibers project, were examined by semiquantitative immunocytochemistry, and compared with those of vasoactive intestinal peptide (VIP). Both in the SCN an...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(94)90585-1
更新日期:1994-11-07 00:00:00
abstract::The cloned vanilloid receptor-1 (VR1) is recognized as a common molecular target for protons, noxious heat, and vanilloids. The presence of VR1 in the dorsal root, trigeminal, and nodose ganglia has been firmly established, but it is unclear in the gut, despite this VR1 may be important for gastric mucosal homeostasis...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(01)02021-3
更新日期:2001-08-17 00:00:00
abstract::This review attempts to explain the symptoms experienced by patients with inflammatory neuropathies by pathophysiological events. The emphasis is not on the primary events that may cause a particular illness but on downstream events taking place in peripheral nerves or muscles. Symptoms that will be discussed include ...
journal_title:Neuroscience letters
pub_type: 杂志文章,评审
doi:10.1016/j.neulet.2014.12.009
更新日期:2015-06-02 00:00:00
abstract::To investigate the relationship between apolipoprotein E (APOE) polymorphisms and the severity of traumatic brain injury (TBI) in acute stage in the cohort of mainland Chinese patients. We prospectively identified admissions to the two neurosurgical departments for head injury. A total of 110 subjects with TBI (80 mal...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.08.082
更新日期:2006-11-13 00:00:00
abstract::We examined the intracellular Ca2+ ([Ca2+]i) elevation evoked by GABA in an 8-day embryonic chick brainstem slice using a Ca imaging technique with Ca green-1 AM. When small quantities of GABA were pressure-ejected on the surface of the slice, the [Ca2+]i elevation was clearly detected. The GABA-induced [Ca2+]i elevat...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2006.10.019
更新日期:2007-01-03 00:00:00
abstract::Motor and sensory neuropathies with the clinical features of HMSN III (Dejerine-Sottas syndrome, DSS) are etiologically related to heterozygous mutations in either peripheral myelin protein-22 (PMP22) or myelin protein zero (MPZ). Heterozygous mutations in either of these two genes are also responsible for other hered...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/s0304-3940(97)00887-2
更新日期:1998-01-02 00:00:00
abstract::1-methyl-4-phenylpyridinium ion (MPP(+)) has been shown to selectively inhibit mitochondrial function and induce a parkinsonism-like syndrome. MPP(+) stimulates the production of reactive oxygen species (ROS) and induces cell death in vitro. In this study, we investigated the protective effects of okadaic acid on MPP(...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/j.neulet.2008.10.103
更新日期:2009-01-09 00:00:00
abstract::A new dipole tracing method, based on a realistic head model, was used to determine dipole locations and vector moments of interictal convexity sharp waves recorded (with conventional EEG technique) from the right fronto-temporal region in a patient with partial complex seizures. When the dipole locations in the head ...
journal_title:Neuroscience letters
pub_type: 杂志文章
doi:10.1016/0304-3940(90)90300-x
更新日期:1990-05-31 00:00:00