Abstract:
:Recent studies revealed the cooperation between peroxisome proliferator-activated receptor gamma (PPARγ) and α-MSH signaling, which results in enhanced melanogenesis in melanocytes and melanoma cells. However, the agonists of PPARα, such as fenofibrate, exert depigmenting effect. Therefore, we aimed to check how the PPARα expression level affects the antimelanogenic activity of fenofibrate and whether PPARα modulates melanogenesis independently of its agonist. To answer these questions, we used three B16 F10-derived cell lines, which varied in the PPARα expression level and were developed by stable transfection with plasmids driving shRNA-based PPARα silencing or overexpression of PPARα-emerald GFP fusion protein. Melanin contents were assessed with electron paramagnetic resonance spectroscopy along with color component image analysis-a novel approach to pigment content characteristics in melanoma cells. B16 F10 wt and Ctrl shRNA lines showed intermediate pigmentation, whereas the pigmentation of the B16 F10-derived cell lines was inversely correlated with the PPARα expression level. We observed that cells overexpressing PPARα were almost amelanotic and cells with reduced PPARα protein level were heavily melanized. Furthermore, fenofibrate down-regulated the melanogenic apparatus (MITF, tyrosinase, and tyrosinase-related proteins) in the cells with the regular PPARα expression level resulting in their visibly lower total melanin content in all the cell lines. From these observations, we conclude that fenofibrate works as a strong depigmenting agent, which acts independently of PPARα, but in an additive fashion. Our results also indicate that alterations in PGC-1a acetylation and expression level might contribute to the regulation of melanogenesis by PPARα and fenofibrate.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Grabacka M,Wieczorek J,Michalczyk-Wetula D,Malinowski M,Wolan N,Wojcik K,Plonka PMdoi
10.1007/s00403-016-1711-2subject
Has Abstractpub_date
2017-04-01 00:00:00pages
141-157issue
3eissn
0340-3696issn
1432-069Xpii
10.1007/s00403-016-1711-2journal_volume
309pub_type
杂志文章abstract::Scleroderma is a heterogeneous disease with a complex etiology. As more information is gained about the underlying mechanisms and the improved classifications of scleroderma subtypes, treatments can be better personalized. Improving scleroderma patients' early diagnosis before end organ manifestations occur should imp...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-016-1647-6
更新日期:2016-09-01 00:00:00
abstract::We investigated the effect of interferon-gamma (IFN-gamma) on skin equivalents. Keratinocytes from involved and uninvolved skin from psoriatic subjects and from healthy subjects were grown on preproduced dermal equivalents (DE) containing fibroblasts from healthy skin or psoriatic lesions. Healthy keratinocytes were a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050146
更新日期:1996-12-01 00:00:00
abstract::Circulating and in situ mononuclear cell subsets were phenotypically characterized during both the acute and convalescent phase of herpes zoster infections in 14 patients. In peripheral blood a significant reduction in the absolute number of Leu 4+ T cells, Leu 2a+ suppressor/cytotoxic T cells, Leu 3a+ helper/inducer ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412622
更新日期:1987-01-01 00:00:00
abstract::The beta-adrenergic receptors, previously shown to be present on the membranes of cultured human epidermal keratinocytes, were found to be functionally coupled to membrane-bound adenylate cyclase. Using membrane preparations, the enzyme could be activated by guanosine triphosphate (GTP), the stable GTP analog GPP(HN)p...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00407740
更新日期:1986-01-01 00:00:00
abstract::Oxidative stress may play a pivotal role in the pathogenesis of psoriasis, an inflammatory/hyperproliferative skin disease characterized by the cutaneous accumulation of neutrophils releasing reactive oxygen species, as revealed in a number of studies. This study was performed to demonstrate the presence of oxidative ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-016-1624-0
更新日期:2016-04-01 00:00:00
abstract::The study aimed at detecting differentially expressed proteins in the stratum corneum of dandruff versus non-dandruff scalps to better understand dandruff aetiology. iTRAQ-based quantitative proteomic analysis revealed a total of 68 differentially expressed biomarkers. A detailed analysis of their known physiological ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-016-1681-4
更新日期:2016-11-01 00:00:00
abstract::In the case of viral acanthomas, the stratum spinosum and granulosum presents ballooned cells which contain all transitional stages from multivesicular bodies (MVB) to keratinosomes. A particularity in condylomata acuminata are the "wagon-wheel" bodies. These structures are typical for the non keratinazed squamous epi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::The use of microalgae in the skin care market is already established although the scientific rationale for their benefit was not clearly defined. In this work, the biological activities of dermatologic interest of the water extract from the microalga Botryococcus braunii (BBWE) were evaluated by a battery of in vitro ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-012-1250-4
更新日期:2012-11-01 00:00:00
abstract::The dermal papilla plays pivotal roles in hair follicle cycle and dermal papilla cells (DPCs) with aggregative behavior have more obviously inductive ability. In the present study, the suppression subtractive hybridization method was employed to identify the differentially expressed genes in dermal papillae cells with...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0583-7
更新日期:2005-09-01 00:00:00
abstract::Histamine is released from mast cells in the skin, causing urticaria and itching. However, little is known about the roles of histamine in development of eczematous lesions in contact dermatitis. Effects of histamine on development of eczematous lesions in contact dermatitis were assessed using histamine-deficient mic...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0569-5
更新日期:2005-08-01 00:00:00
abstract::Exposure of hairless mice to the light of a germicidal lamp (254 nm) under conditions which are known to induce epidermal DNA synthesis, cell proliferation, and polyamine metabolism produced a marked increase of polyamine excretion in the urine which lasted for many days. The increase was about the same for free and a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417146
更新日期:1981-01-01 00:00:00
abstract::The rhino mouse comedolytic model and the hairless mouse photoaging model are established animal models for screening the in vivo activity of retinoids. However, the expression of the retinoic acid receptors (RARs) and cellular retinoic acid-binding proteins (CRABPs), known to regulate retinoid activity, is not comple...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00373433
更新日期:1995-01-01 00:00:00
abstract::To evaluate the proliferative activity of benign, borderline and malignant cutaneous melanocytic neoplasms, 30 cases of malignant melanoma (MM) and 41 cases of naevi were studied by immunostaining using a monoclonal antibody against proliferating cell nuclear antigen (PCNA). PCNA is a nuclear antigen expressed in the ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00372033
更新日期:1992-01-01 00:00:00
abstract::We investigated the effects of 1-4% ointments of metronidazole and tinidazole (derivatives of nitroimidazole) on models of inflammatory dermatitis evoked by antigen, hapten and monoclonal anti-dinitrophenol (DNP) IgE antibody in mice. Metronidazole and tinidazole ointments (1) suppressed the late-phase reaction (LPR) ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-002-0381-4
更新日期:2003-03-01 00:00:00
abstract::Transcutaneous oxygen pressure (TcPO2) and skin blood flow (as evaluated by laser doppler) (LD) were investigated in 24 systemic sclerosis (SSc) patients in sclerotic skin (dorsal aspect of the hand) and non-sclerotic skin (interscapular region) and in 24 controls matched for sex and age for the same sites. The two pa...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00376614
更新日期:1991-01-01 00:00:00
abstract::GM-CSF is known primarily as a hematopoietic growth factor, but it has also been shown to inhibit mast cell differentiation in vitro. In order elucidate the mechanisms involved, we investigated the effects of GM-CSF in vitro on the differentiation of human leukemic mast cells (HMC-1 cells) and normal cord blood-derive...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030100225
更新日期:2001-05-01 00:00:00
abstract::Dopamine causes reflex erythema, central blanching, and piloerection depending on the dose and the type of application wheal reaction. Intracutaneous application shows from 1.5 gamma/0.1 ml wheal formation, erythema, piloerection, and blanching combined with increased heat radiation from the skin surface (AGA thermovi...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00412863
更新日期:1979-08-01 00:00:00
abstract::The injured skin produces a number of mediators that directly or indirectly modulate cell chemotaxis, migration, proliferation, and angiogenesis. Components of the kinin pathway including the kinin B1 receptor (B1R) have been found to occur in the human skin, but information about its role on keratinocyte biology is s...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-015-1595-6
更新日期:2015-11-01 00:00:00
abstract::The expression and location of the Ki-67 antigen was investigated in 62 basal cell carcinomas (BCC) using immunostaining techniques (PAP and APAAP) on cryostat sections. The tumor samples were classified into three groups according to microarchitecture (nodular, superficial or fibrosing). The Ki-67 growth fraction dis...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371599
更新日期:1993-01-01 00:00:00
abstract::Antimicrobial peptides and proteins (AMPs) play important roles in skin immune defense due to their capacity to inhibit growth of microbes. During intrauterine life, the skin immune system has to acquire the prerequisites to protect the newborn from infection in the hostile environment after birth, which includes the ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-013-1340-y
更新日期:2013-08-01 00:00:00
abstract::Glycerol, widely used as humectant, is known to protect against irritants and to accelerate recovery of irritated skin. However, most studies were done with topical formulations (i.e. emulsions) containing glycerol in relatively high amounts, preventing drawing conclusions from direct effects. In this study, acute che...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-1021-z
更新日期:2010-08-01 00:00:00
abstract::Localized scleroderma (LoSc) is rare connective tissue disease that manifests with inflammation and fibrosis of the skin. Depending on the LoSc subtype, adjacent structures such as subcutaneous tissue, fascia, muscles, bones may be affected. The hallmark of fibrosis is tissue remodelling with excess deposition of extr...
journal_title:Archives of dermatological research
pub_type: 杂志文章,评审
doi:10.1007/s00403-019-01991-0
更新日期:2020-07-01 00:00:00
abstract::Besides its role in small-cell carcinoma of the lung, elevated serum levels of neuron-specific enolase (NSE) have recently been reported to be associated with autoimmune rheumatic disorders such as systemic sclerosis. Serum NSE seems to correlate with disease activity as well as Rodnan skin score. The aim of the study...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-004-0495-y
更新日期:2004-09-01 00:00:00
abstract::Mite antigens play important roles in the pathogenesis of atopic dermatitis (AD). We recently developed a novel air cleaner (KC-850U) using charged plasma cluster ions to eliminate a variety of allergens from house environments. The present work demonstrates the ability of KC-850U to decrease the symptoms of AD induce...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-010-1095-7
更新日期:2011-07-01 00:00:00
abstract::Enhanced susceptibility to viral infections has been reported repeatedly in atopic dermatitis (AD). A difference in the capacity to produce interferons (IFN) in response to viral antigens may be the cause. In the present study we investigated the in vitro IFN production of leukocytes from AD patients in response to di...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00585921
更新日期:1987-01-01 00:00:00
abstract::Psoriasis was initially considered to represent a disease of abnormal epidermal keratinocyte proliferation. Proliferation of keratinocytes is restricted by apoptotic cell death to maintain a constant thickness of epidermis. Nucleosomes are mainly released by apoptotic cells. Tumor necrosis factor-α (TNF-α) is an impor...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-013-1347-4
更新日期:2013-08-01 00:00:00
abstract::In skin inflammation, vascular endothelial growth factor (VEGF) and CXCL-8/IL-8 play an important role and are produced by activated keratinocytes. Extracts from Ginkgo biloba leaves (GBE), widely used in phytotherapy, have been reported to exert antioxidant and anti-inflammatory properties in the skin. We therefore e...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-0979-x
更新日期:2010-04-01 00:00:00
abstract::In the present study, attempts were made to clarify the role of an elastase-like enzyme in Arthus reaction by using rabbit-peritoneal polymorphonuclear leukocytes (PMNs). It was first confirmed that the injection of large amounts of PMNs with antibody can increase tissue damage in reversed Arthus reaction. The five va...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00413357
更新日期:1984-01-01 00:00:00
abstract::The goal of this study is to investigate commercially available cosmetics (foundations, skin care creams) which also claim to include a sun protection factor (SPF). Are these products, which are not considered sunscreen products, helpful or could they be harmful? Using an in vitro method, we tested the effectiveness o...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-0974-2
更新日期:2009-09-01 00:00:00
abstract::The treatment of drug-induced toxic epidermal necrolysis (TEN) remains unsatisfactory. Intravenous immunoglobulins (IVIg) and intravenous cyclosporin A (CsA) have shown some efficacy in short series of patients. We assessed the effects of IVIg and CsA on TEN lesional and apparently uninvolved skin using standard histo...
journal_title:Archives of dermatological research
pub_type: 临床试验,杂志文章
doi:10.1007/s00403-005-0607-3
更新日期:2005-12-01 00:00:00