Abstract：
:The injured skin produces a number of mediators that directly or indirectly modulate cell chemotaxis, migration, proliferation, and angiogenesis. Components of the kinin pathway including the kinin B1 receptor (B1R) have been found to occur in the human skin, but information about its role on keratinocyte biology is still scarce. Our aim was to determine whether stimulation of B1R causes the secretion of IL-4 and/or VEGF from human keratinocytes and to evaluate the role of the B1R agonist Lys-des[Arg(9)]bradykinin and IL-4 on various stages of angiogenesis, such as cell migration, proliferation, and release of metalloproteases. By using ELISA and Western blotting, we showed that HaCaT keratinocytes stimulated with the B1R agonist release IL-4 and VEGF. Stimulation of B1R also caused transient c-JunN-terminal kinase phosphorylation and JunB nuclear translocation, transcription factor that regulates IL-4 expression. The 3D-angiogenesis assay, performed on spheroids of EA.hy923 endothelial cells embedded in a collagen matrix, showed that their cumulative sprout area increased significantly following stimulation with either IL-4 or B1R agonist. Furthermore, these ligands produced significant endothelial cell migration and release of metalloproteases 2 and 9, but did not increase endothelial cell proliferation as measured by 5-bromo-2'-deoxyuridine incorporation. Our results provide experimental evidence that establishes IL-4 and B1R agonist as important angiogenic factors of relevance for skin repair.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Mejia AJ,Matus CE,Pavicic F,Concha M,Ehrenfeld P,Figueroa CDdoi
10.1007/s00403-015-1595-6subject
Has Abstractpub_date
2015-11-01 00:00:00pages
803-17issue
9eissn
0340-3696issn
1432-069Xpii
10.1007/s00403-015-1595-6journal_volume
307pub_type
杂志文章abstract：:The hair cycling pattern differs between humans and mice: for the former it is mosaic, while the latter show a wave pattern. We focused on the presence or absence of synchronization of the hair cycle based on the distribution of Merkel cells. The three-dimensional distribution and number of Merkel cells in the skin at...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050474
更新日期：2000-04-01 00:00:00
abstract：:Skin fibrosis has been reported in Borrelia burgdorferi infection in Europe, but has been questioned by several authors. The objective of the present study was to examine the interaction of skin fibroblasts with B. burgdorferi sensu stricto B31 (BB) and B. afzelii (BA) in vitro by electron microscopy. We also determin...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1797-1
更新日期：2018-03-01 00:00:00
abstract：:Antihistamines are the first-line treatment for chronic urticaria (CU). However, some CU patients are relatively refractory to antihistamines. The mechanism underlying the interindividual variation is still unknown. The α-chain of the high-affinity IgE receptor is crucial to the IgE-mediated allergic response. The pre...
journal_title：Archives of dermatological research
pub_type： 临床试验,杂志文章
doi：10.1007/s00403-014-1525-z
更新日期：2015-03-01 00:00:00
abstract：:The function of microphages has been studied in two patients with chronic pyoderma vegetans by in vitro determination of phagocytosis as well as chemotaxis. The results showed a striking decrease in the chemotactic activity of the neutrophil granulocytes, a reduced phagocytosis of Candida albicans and Staphylococcus a...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：
更新日期：1978-01-01 00:00:00
abstract：:Human epidermal keratinocytes were grown in culture and the uptake of hematoporphyrin derivatives (HPDs) used in photodynamic therapy was estimated. Keratinocytes loaded with HPDs were irradiated with laser light of 632 nm generated by a helium-neon laser and cell toxicity was determined by the trypan blue exclusion t...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00510086
更新日期：1989-01-01 00:00:00
abstract：:The mechanistic target of rapamycin (mTOR) is involved in the regulation of cellular growth, proliferation, lipid synthesis, and protein translation. The mTOR pathway involves two complexes: the mechanistic target of rapamycin complex 1 (mTORC1) and the mechanistic target of rapamycin complex 2 (mTORC2). Both mTOR com...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/s00403-014-1480-8
更新日期：2014-12-01 00:00:00
abstract：:Studies aimed at understanding the pathology, genetics, and therapeutic response of vitiligo rely on asking a single question about 'physician-diagnosed' vitiligo on surveys to identify subjects for research. However, this type of self-reporting is not sufficient. Our objective was to determine if the patient-administ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-014-1515-1
更新日期：2015-01-01 00:00:00
abstract：:Langerhans cells appear to be critical for IgE-mediated allergen capture and presentation in human atopic dermatitis. The present study sought to determine whether epidermal (i.e Langerhans cells) and dermal dendritic cells in the skin of dogs with atopic dermatitis are hyperplastic and expressed surface IgE. Frozen s...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505260
更新日期：1996-09-01 00:00:00
abstract：:The presence, distribution and cellular localization of tumour necrosis factor-alpha (TNF alpha) were investigated in normal human and murine epidermis using immunohistological and immunoelectron microscopic methods with monoclonal and polyclonal antibodies. The immunostaining revealed an intercellular plasma membrane...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00372709
更新日期：1992-01-01 00:00:00
abstract：:Tropospheric ozone (O3) is a source of oxidative stress. This study examined the ability of a topical antioxidant (WEL-DS) to inhibit O3-mediated damage in a human epidermal skin model. Four groups of tissues (N = 24) were compared: Group 1 (control) were untreated and unexposed; Group 2 were untreated and exposed to ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-020-02083-0
更新日期：2020-05-08 00:00:00
abstract：:This study uses light and electron microscopes to gain a better knowledge of the interactions of calcium hydroxylapatite filler with the connective tissue of the skin and the modifications of the human deep dermis, after 2 months of treatment. Some morphological evidences of this observational study of filler treated ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1734-3
更新日期：2017-07-01 00:00:00
abstract：:Psoriasis is now recognized as an immune-mediated inflammatory dermatosis with increased risk for metabolic syndrome, its individual components, and cardiovascular disease. We quantitatively estimated malondialdehyde (MDA), lipoprotein-a (LP-a), lipoprotein ratios, comprehensive lipid tetrad index (CLTI), and atheroge...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-019-01896-y
更新日期：2019-05-01 00:00:00
abstract：:Two male patients with longstanding contact sensitivity to chromium were treated with PUVA. One patient, suffering from concomitant photosensitivity, reacted very favorably; his skin lesions cleared and light tolerance increased. This was paralleled by a decrease in the photopatch test reactivity and by the extinction...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00403929
更新日期：1981-01-01 00:00:00
abstract：:Interferon-alpha combined with retinoid or PUVA is used for the treatment of cutaneous T-cell lymphoma. Anti-IFN-alpha antibodies (IFN ab) occur regularly during IFN-alpha treatment. We investigated the incidence of neutralizing and binding IFN ab and analysed their relationship with clinical and immunological paramet...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505252
更新日期：1996-08-01 00:00:00
abstract：:Heparin-binding EGF-like growth factor (HB-EGF) and amphiregulin (AREG) are the members of EGF family that bind to common EGF receptor (EGFR) in the epidermis. However, the role of these two growth factors in epidermal hyperplasia of psoriasis has not been established. On the other hand, CD4+ T cells are responsible f...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-007-0809-y
更新日期：2008-01-01 00:00:00
abstract：:The incidence of cutaneous malignant melanoma continues to rise. Strategies to reduce mortality in this situation include improving therapy, encouraging earlier diagnosis, and promoting prevention. New developments such as epiluminescence, sentinel node biopsy, arterial limb perfusion and the use of vaccines are discu...
journal_title：Archives of dermatological research
pub_type： 杂志文章,评审
doi：10.1007/BF00370712
更新日期：1994-01-01 00:00:00
abstract：:In human skin, epidermal differentiation occurs in two ways: normal differentiation, characterized by keratin 10 expression, and alternative differentiation. Alternative differentiation can be hyperproliferation-associated differentiation (expression of keratin 16) or the reinduction of an embryonic type of differenti...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/pl00007455
更新日期：2000-01-01 00:00:00
abstract：:Paraneoplastic pemphigus patients (PNP) develop a group of autoantibodies, among which those against envoplakin and periplakin are almost always found. Epitope mapping has indicated that the linker subdomains of the proteins harbor the major antigenic sites recognized by PNP sera. In order to detect specific autoantib...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-008-0901-y
更新日期：2009-10-01 00:00:00
abstract：:The topical application of a mixture of sugar and povidone--iodine (PI) has been reported to accelerate the healing of cutaneous wounds and ulcers by promoting re-epithelialization and granulation tissue formation as well as having an anti-microbial effect. To clarify the mechanisms accounting for the efficacy of a 70...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-006-0683-z
更新日期：2006-09-01 00:00:00
abstract：:Flushing in rosacea has been investigated by means of (a) pharmacological inhibition of some possible chemical mediators and (b) titration of bradykinin as a possible effector directly in the blood. Clonidine-inhibited flushing was seen in all patients (mean 45%), other drugs had poorer results. Bradykinin increased i...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00509061
更新日期：1982-01-01 00:00:00
abstract：:Atopic dermatitis (AD) is a chronic inflammatory skin disease that affects the patients' quality of life greatly often from a very young age. Its worldwide incidence in children and adults varies, but it is usually among the first ten causes of dermatological consultation worldwide. There is a wide variety of treatmen...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-017-1791-7
更新日期：2018-01-01 00:00:00
abstract：:A low molecular mass cysteine proteinase inhibitor was found and isolated from bovine hoof. Purification of the inhibitor to homogeneity was performed by carboxymethyl-papain-sepharose affinity and DE-52 ion exchange chromatographies. From 15 g of bovine hoof, 0.25 mg of cysteine proteinase inhibitor was isolated. It ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF02505240
更新日期：1996-07-01 00:00:00
abstract：:Recent evidence indicates that the biologically active metabolite of vitamin D3, 1 alpha,25-dihydroxyvitamin D3 [1 alpha,25(OH)2D3], has an effect on the regulation of the immune response. We investigated whether topical treatment of mice with 1 alpha,25(OH)2D3 influences the contact hypersensitivity (CHS) response to...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00412982
更新日期：1989-01-01 00:00:00
abstract：:This study concerns 57 patients who fulfilled histological criteria for the diagnosis allergic vasculitis. For 37 of these patients, biopsy specimen were available from lesional and adjacent non-lesional skin. Histological signs of vasculitis were found at both sites, but in clinically normal skin the perivascular inf...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00406538
更新日期：1980-01-01 00:00:00
abstract：:Angiosarcoma, a malignant tumor of vascular endothelial cell origin, is a lethal disease for which complete cure is rarely seen. The objective of this study was to determine the efficiency of photodynamic therapy (PDT) as a new treatment for angiosarcoma. PDT (630 nm, 25 J/cm2) using Photofrin for a mouse angiosarcoma...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-007-0820-3
更新日期：2008-04-01 00:00:00
abstract：:Human alpha 1(I) and alpha 1(III) procollagen mRNA expression in skin tissue from 15 systemic sclerosis (SSc) patients and from 7 normal control subjects was quantitatively analyzed using in situ hybridization. The grains accumulating in each area, representing procollagen mRNA expression per cell, were counted. To no...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050458
更新日期：1999-11-01 00:00:00
abstract：:The phospholipids of the skin are difficult to quantify because they represent only a small fraction of the skin tissue. In this study, 31P nuclear magnetic resonance, which permits precise profiling of these phospholipids, was used to compare the phospholipids of upper eyelid epidermal and dermal lipid extracts (n = ...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s004030050308
更新日期：1998-06-01 00:00:00
abstract：:No previous studies have convincingly linked sweating disturbance with the subsequent development of lichen planus (LP). Therefore, we investigated whether sweating disturbance could be specifically detected in LP lesions and how it could trigger lesion development. We utilized the impression mold technique (IMT), whi...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-018-1882-0
更新日期：2019-01-01 00:00:00
abstract：:Solar radiation in the UVB range is absorbed primarily by the epidermal DNA where characteristic photodamage results in altered immune responses and mutagenic lesions. UVB exposure of the skin results in a profound upregulation of the anti-inflammatory cytokine, IL-10 and suppression of contact hypersensitivity (CHS)....
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/s00403-005-0634-0
更新日期：2006-03-01 00:00:00
abstract：:Fingernail growth was studied in 28 patients who were receiving systemic treatment with the aromatic retinoid, Ro 10-9359 known as Tigason (etretinate; AR). A group of 15 patients with psoriasis was compared with a group of 13 patients with other dermatoses which are known to have a good response to AR therapy. In the...
journal_title：Archives of dermatological research
pub_type： 杂志文章
doi：10.1007/BF00414112
更新日期：1985-01-01 00:00:00
