Abstract:
:GM-CSF is known primarily as a hematopoietic growth factor, but it has also been shown to inhibit mast cell differentiation in vitro. In order elucidate the mechanisms involved, we investigated the effects of GM-CSF in vitro on the differentiation of human leukemic mast cells (HMC-1 cells) and normal cord blood-derived mast cells (CBMC) under the influence of SCF, NGF, and fibroblast supernatant (FS). Under all culture conditions, GM-CSF induced a dose- and time-dependent reduction in intracellular histamine levels, tryptase activity, and numbers of cells immunoreactive for c-Kit and FcepsilonRIalpha. This effect leveled off between 10-100 ng/ml and after 4 days of culture. There was an associated decrease in mRNA expression for c-kit, FcepsilonRIalpha and tryptase. In contrast, no significant changes in the expression of the NGF receptor TrkA were noted under the same conditions. The GM-CSF receptor was found in HMC-1 cells and CBMC at both the mRNA and protein levels, but its expression decreased during culture with FS, and even more markedly during culture with GM-CSF. GM-CSF thus selectively inhibits in vitro induction and/or upregulation of all major mast cell characteristics in HMC-1 cells and CBMC irrespective of the growth factors present, and a concomitant downregulation of GM-CSF receptors can counteract these effects. GM-CSF may therefore function as a regulatory factor in mast cell growth and differentiation under normal and pathological conditions.
journal_name
Arch Dermatol Resjournal_title
Archives of dermatological researchauthors
Welker P,Grabbe J,Zuberbier T,Grützkau A,Henz BMdoi
10.1007/s004030100225keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
249-58issue
5eissn
0340-3696issn
1432-069Xjournal_volume
293pub_type
杂志文章abstract::The penetration and permeation of the recombinant protein plasminogen activator inhibitor type 2 (PAI-2) in two formulations, one containing a penetration enhancer, into the psoriatic and uninvolved skin of eight patients with plaque-type psoriasis were investigated. Penetration and permeation of PAI-2 were measured b...
journal_title:Archives of dermatological research
pub_type: 临床试验,杂志文章
doi:10.1007/s00403-003-0425-4
更新日期:2003-11-01 00:00:00
abstract::Immigration of neutrophils into the epidermis is a hallmark of several so-called neutrophilic dermatoses. In this study we used a frozen section adhesion assay to study the impact of different activation procedures as well as of CD11 integrins on cell-cell adhesion between neutrophils and epidermal cells. Serial secti...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050263
更新日期:1997-11-01 00:00:00
abstract::Exposure of hairless mice to the light of a germicidal lamp (254 nm) under conditions which are known to induce epidermal DNA synthesis, cell proliferation, and polyamine metabolism produced a marked increase of polyamine excretion in the urine which lasted for many days. The increase was about the same for free and a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417146
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journal_title:Archives of dermatological research
pub_type: 临床试验,杂志文章
doi:10.1007/BF00403843
更新日期:1980-01-01 00:00:00
abstract::The history of 188 caucasian patients with atopic dermatitis (AD) and of 2,151 family members has been analyzed. Of the AD patients 48% suffered from respiratory atopy (36% rhinitis, 28% asthma, and 15% both). AD showed by far the earliest onset of all atopic diseases: 50% of our patients had skin lesions before the a...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00493466
更新日期:1990-01-01 00:00:00
abstract::Botulinum toxin type A (BoNT-A) is an easy and fast treatment for gingival smile (GS). The objective of the study was to compare the reduction of gingival exposure using three different doses of abobotulinumtoxinA (ABO) in patients with different severities of anterior GS, and to assess treatment safety and patients' ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-020-02096-9
更新日期:2020-07-28 00:00:00
abstract::We previously showed that the homeodomain protein HOXC13 is involved in the expression control of the early human hair keratin genes hHa5 and hHa2, which contain specific HOXC13 binding sites in their proximal promoters. Hox specificity is generally thought to be enhanced by the interaction with members of the TALE su...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-005-0623-3
更新日期:2006-02-01 00:00:00
abstract::Previous studies in mice have shown that dehydroepiandrosterone (DHEA) increases the production of Th1-associated lymphokines, and of interleukin-2 (IL-2) and interferon-gamma (IFN-gamma), by lymphocytes. However, there are no reports concerning the effect of DHEA on the production of Th2-associated lymphokines, IL-4 ...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050213
更新日期:1997-06-01 00:00:00
abstract::To have as further insight into the localizations of immune deposits in the skin the reversed passive and the passive Arthus reactions were carried out in the foot-pads skin of guinea-pigs using horseradish peroxidase as antigen. The transient localizations of immune deposits in the papillary dermis and in the epiderm...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00404457
更新日期:1980-01-01 00:00:00
abstract::Total osmotic pressure does not differ significantly in suction blister fluid or in serum of healthy persons from blister fluid, suction blister fluid or serum of patients with dermatitis herpetiformis, bullous contact dermatitis and pemphigus vulgaris. The average values reach about 293 mOsmol/kg. This results from d...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::T Cells from 14 patients in the active stage of bullous pemphigoid and 10 patients in the inactive stage of the disease were studied with Leu-1, Leu-2, and Leu-3 monoclonal antibodies. The proportions of total T cells and T cells expressing either helper or suppressor phenotype in the peripheral blood of patients were...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00509086
更新日期:1985-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050146
更新日期:1996-12-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-009-0974-2
更新日期:2009-09-01 00:00:00
abstract::Human eccrine sweat glands were embedded in Lowicryl K4M. Cytokeratin proteins and blood group H antigen were localized by applying a postembedding immunogold method using a monoclonal antikeratin antibody and the lectin Ulex europaeus I. The antikeratin antibody labeled intermediate filaments in the secretory coil an...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00505639
更新日期:1990-01-01 00:00:00
abstract::1. Suction blisters on the skin of rats, obtained with a negative pressure of 150 mm Hg, show a close similarity to human suction blisters, based on histological, electrolyte and protein findings. 2. Pharmacokinetic experiments with inulin and antipyrine have proved that the movement of these low protein binding drugs...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00446949
更新日期:1978-12-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-014-1460-z
更新日期:2014-09-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00371647
更新日期:1990-01-01 00:00:00
abstract::The impact of search strategies on systematic reviews (SR) of atopic dermatitis (AD) is unknown. The purpose of this review was to evaluate search strategies used in SR of AD and their impact on the frequency of manuscripts identified. MEDLINE and EMBASE were searched for SR related to AD. Simulations were performed b...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-020-02165-z
更新日期:2020-11-22 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00372058
更新日期:1991-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-010-1071-2
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050304
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00406686
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s00403-015-1582-y
更新日期:2015-10-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00417384
更新日期:1981-01-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030050168
更新日期:1997-02-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030100216
更新日期:2001-04-01 00:00:00
abstract::Exposure to a dry environment leads to depletion of water from the peripheral stratum corneum layers in a process dependent on the relative humidity (RH) and the intrinsic properties of the tissue. We hypothesized that by modulating the water content of the stratum corneum in the surface layers, RH effects the rate of...
journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/s004030100257
更新日期:2001-09-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
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更新日期:2004-09-01 00:00:00
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journal_title:Archives of dermatological research
pub_type: 杂志文章
doi:10.1007/BF00418573
更新日期:1979-11-01 00:00:00
abstract::Propylene glycol (propane-1,2-diol) is the only diol widely used in dermatology. Pentane-1,5-diol is mainly used as a plasticizer in cellulose products and adhesives, in dental composites and in brake fluid compositions and as a preservative for grain. However, pentane-1,5-diol is also an effective solvent, water-bind...
journal_title:Archives of dermatological research
pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00