Abstract:
:Circular RNAs (circRNAs) have been identified in diverse eukaryotic species and are characterized by RNA backsplicing events. Current available methods for circRNA identification are able to determine the start and end locations of circRNAs in the genome but not their full-length sequences. In this study, we developed a method to assemble the full-length sequences of circRNAs using the backsplicing RNA-Seq reads and their corresponding paired-end reads. By applying the method to an rRNA-depleted/RNase R-treated RNA-Seq dataset, we for the first time identified full-length sequences of nearly 3,000 circRNAs in rice. We further showed that alternative circularization of circRNA is a common feature in rice and, surprisingly, found that the junction sites of a large number of rice circRNAs are flanked by diverse non-GT/AG splicing signals while most human exonic circRNAs are flanked by canonical GT/AG splicing signals. Our study provides a method for genome-wide identification of full-length circRNAs and expands our understanding of splicing signals of circRNAs.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Ye CY,Zhang X,Chu Q,Liu C,Yu Y,Jiang W,Zhu QH,Fan L,Guo Ldoi
10.1080/15476286.2016.1245268subject
Has Abstractpub_date
2017-08-03 00:00:00pages
1055-1063issue
8eissn
1547-6286issn
1555-8584journal_volume
14pub_type
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