Antisense-mediated modulation of splicing: therapeutic implications for Duchenne muscular dystrophy.

abstract：:The human genome contains some thousands of long non coding RNAs (lncRNAs). Many of these transcripts are presently considered crucial regulators of gene expression and functionally implicated in developmental processes in Eukaryotes. Notably, despite a huge number of lncRNAs are expressed in the Central Nervous Syste...

journal_title：RNA biology

authors： Bevilacqua V,Gioia U,Di Carlo V,Tortorelli AF,Colombo T,Bozzoni I,Laneve P,Caffarelli E

更新日期：2015-01-01 00:00:00

abstract：:Population differences observed for complex traits may be attributed to the combined effect of socioeconomic, environmental, genetic and epigenetic factors. To better understand population differences in complex traits, genome-wide genetic and gene expression differences among ethnic populations have been studied. Her...

journal_title：RNA biology

authors： Huang RS,Gamazon ER,Ziliak D,Wen Y,Im HK,Zhang W,Wing C,Duan S,Bleibel WK,Cox NJ,Dolan ME

更新日期：2011-07-01 00:00:00

abstract：:Fragile X-associated tremor/ataxia syndrome (FXTAS) is a recently identified neurodegenerative disorder affecting older adult males with premutation alleles of the fragile X mental retardation 1 (FMR1) gene. The principal clinical features of FXTAS include progressive intention tremor, gait ataxia, parkinsonism, and a...

journal_title：RNA biology

authors： Tassone F,Iwahashi C,Hagerman PJ

更新日期：2004-07-01 00:00:00

abstract：:Oncogenic viruses are associated with approximately 15% of human cancers. In viral infections, microRNAs play an important role in host-pathogen interactions. miR-21 is a highly conserved non-coding RNA that not only regulates the development of oncogenic viral diseases, but also responds to the regulation of intracel...

journal_title：RNA biology

authors： He G,Ding J,Zhang Y,Cai M,Yang J,Cho WC,Zheng Y

更新日期：2021-01-27 00:00:00

abstract：:Significant structural and mechanistic similarities between the spliceosomal snRNAs and catalytically critical domains of self-splicing group II introns have led to the hypothesis that the spliceosomes and group II introns may be evolutionarily related. We have previously shown that in vitro-transcribed, protein-free ...

journal_title：RNA biology

authors： Jaladat Y,Zhang B,Mohammadi A,Valadkhan S

更新日期：2011-05-01 00:00:00

abstract：:With a minimal (250-400 nt), non-protein-coding, circular RNA genome, viroids rely on sequence/structural motifs for replication and colonization of their host plants. These motifs are embedded in a compact secondary structure whose elucidation is crucial to understand how they function. Viroid RNA structure has been ...

journal_title：RNA biology

authors： López-Carrasco A,Flores R

更新日期：2017-08-03 00:00:00

abstract：:Alternative splicing (AS) in response to changing external conditions often requires alterations in the ability of sequence-specific RNA-binding proteins to bind to cis-acting sequences in their target pre-mRNA. While daily oscillations in AS events have been described in several organisms, cis-acting sequences that c...

journal_title：RNA biology

authors： Goldammer G,Neumann A,Strauch M,Müller-McNicoll M,Heyd F,Preußner M

更新日期：2018-01-01 00:00:00

abstract：:Telomeres, protective nucleoprotein structures located at the ends of eukaryotic chromosomes and until recently regarded as transcriptionally silent, are now known to be transcribed into non-coding RNA molecules called TERRA. The function(s) of TERRA in telomere metabolism is now the subject of intense study given the...

journal_title：RNA biology

authors： Caslini C

更新日期：2010-01-01 00:00:00

abstract：:Ribonuclease P (RNase P) is a ribonucleoprotein comprised of a catalytic RNA subunit and one or several protein subunits. RNase P is best known for its role in 5'-processing of tRNA precursors. RNase P enzymes from almost all forms of life, including protein-synthesizing organelles, contain an RNase P with a conserved...

journal_title：RNA biology

authors： Ellis JC,Brown JW

更新日期：2009-09-01 00:00:00

abstract：:Traditionally the principles of protein folding in vivo have been obtained largely from molecular chaperone studies. Through extensive studies on molecular chaperones, it becomes clear that most proteins can fold without their assistance in vivo, suggesting the existence of other chaperone types and mechanisms. Since ...

journal_title：RNA biology

authors： Choi SI,Ryu K,Seong BL

更新日期：2009-01-01 00:00:00

abstract：:microRNAs (miRNAs) play significant regulatory roles in gene expression at the post-transcriptional level. This includes modulating processes such as development, immunity, cancer, and host-pathogen interactions. It was recently shown that the phylogenetically deeply conserved miRNA, miR-8, plays a role in maintaining...

journal_title：RNA biology

authors： Etebari K,Asgari S

更新日期：2013-08-01 00:00:00

abstract：:Translation begins at AUG, GUG, or UUG codons in bacteria. Start codon recognition occurs in the P site, which may help explain this first-position degeneracy. However, the molecular basis of start codon specificity remains unclear. In this study, we measured the codon dependence of 30S•mRNA•tRNAfMet and 30S•mRNA•tRNA...

journal_title：RNA biology

authors： Roy B,Liu Q,Shoji S,Fredrick K

更新日期：2018-01-01 00:00:00

abstract：:MicroRNAs (miRNAs) are key regulators of gene expression and play critical roles in modulating metabolism, development and physiology in animals and plants. miRNA levels are transcriptionally and post-transcriptionally controlled for their proper function. Recent studies have shown that RNA-binding proteins play impor...

journal_title：RNA biology

authors： Ren G,Yu B

更新日期：2012-12-01 00:00:00

abstract：:Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a multifunctional RNA-binding protein that is involved in many different processes, such as regulation of transcription, translation, and RNA stability. We have previously characterized hnRNP L as a global regulator of alternative splicing, binding to CA-repeat, a...

journal_title：RNA biology

authors： Rossbach O,Hung LH,Khrameeva E,Schreiner S,König J,Curk T,Zupan B,Ule J,Gelfand MS,Bindereif A

更新日期：2014-01-01 00:00:00

abstract：:This review emphasizes the function and regulation of the Csr regulatory system in the human enteropathogen Yersinia pseudotuberculosis and compares its features with the homologous Csr/Rsm systems of related pathogens. The Csr/Rsm systems of eubacteria form a complex regulatory network in which redundant non-translat...

journal_title：RNA biology

authors： Heroven AK,Böhme K,Dersch P

更新日期：2012-04-01 00:00:00

abstract：:The RIG-I-like receptors (RLRs)--RIG-I, MDA5, and LGP2--detect intracellular pathogenic RNA and elicit an antiviral immune response during viral infection. The protein architecture of the RLR family consists of multiple functional domains, including N-terminal Caspase Activation and Recruitment Domains (CARDs) for sig...

journal_title：RNA biology

authors： Luo D

更新日期：2014-01-01 00:00:00

abstract：:Type II CRISPR-Cas9 systems require a small RNA called the trans-activating CRISPR RNA (tracrRNA) in order to function. The prediction of these non-coding RNAs in prokaryotic genomes is challenging because they have dissimilar structures, having short stems (3-6 bp) and non-canonical base-pairs e.g. G-A. Much of the t...

journal_title：RNA biology

authors： Chyou TY,Brown CM

更新日期：2019-04-01 00:00:00

abstract：:Circular RNAs (circRNAs) have been identified in diverse eukaryotic species and are characterized by RNA backsplicing events. Current available methods for circRNA identification are able to determine the start and end locations of circRNAs in the genome but not their full-length sequences. In this study, we developed...

journal_title：RNA biology

authors： Ye CY,Zhang X,Chu Q,Liu C,Yu Y,Jiang W,Zhu QH,Fan L,Guo L

更新日期：2017-08-03 00:00:00

abstract：:Wobble uridines (U34) are generally modified in all species. U34 modifications can be essential in metazoans but are not required for viability in fungi. In this review, we provide an overview on the types of modifications and how they affect the physico-chemical properties of wobble uridines. We describe the molecula...

journal_title：RNA biology

authors： Schaffrath R,Leidel SA

更新日期：2017-09-02 00:00:00

abstract：:High concentrations (> 100 µM) of the ribonucleoside analog 4-thiouridine (4sU) is widely used in methods for RNA analysis like photoactivatable-ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) and nascent messenger (m)RNA labeling (4sU-tagging). Here, we show that 4sU-tagging at low concentrati...

journal_title：RNA biology

authors： Burger K,Mühl B,Kellner M,Rohrmoser M,Gruber-Eber A,Windhager L,Friedel CC,Dölken L,Eick D

更新日期：2013-10-01 00:00:00

abstract：:Human Enterovirus 71 (EV71) is an emerging pathogen of infectious disease and a serious threat to public health. Currently, there are no antivirals or vaccines to slow down or prevent EV71 infections, thus underscoring the urgency to better understand mechanisms of host-enterovirus interactions. EV71 uses a type I int...

journal_title：RNA biology

authors： Levengood JD,Tolbert M,Li ML,Tolbert BS

更新日期：2013-07-01 00:00:00

abstract：:microRNAs (miRNAs) are critical regulators of gene expression. For elucidating functional roles of miRNAs, it is critical to identify their direct targets. There are debates about whether pulldown of biotinylated miRNA mimics can be used to identify miRNA targets or not. Here we show that biotin-labelled miR-34a can b...

journal_title：RNA biology

authors： Awan HM,Shah A,Rashid F,Wei S,Chen L,Shan G

更新日期：2018-01-02 00:00:00

abstract：:"What does one do when the giants pass away?" remarked a very famous microbiologist to me, soon after the death of Carl Woese in late 2012. I teach microbiology and introductory cell and molecular biology at a small undergraduate institution; Woese's example and contributions have long been part of every class I teach...

journal_title：RNA biology

authors： Martin M

更新日期：2014-01-01 00:00:00

abstract：:The development of rigorous molecular taxonomy pioneered by Carl Woese has freed evolution science to explore numerous cellular activities that lead to genome change in evolution. These activities include symbiogenesis, inter- and intracellular horizontal DNA transfer, incorporation of DNA from infectious agents, and ...

journal_title：RNA biology

authors： Shapiro JA

更新日期：2014-01-01 00:00:00

abstract：:Long noncoding RNAs (lncRNAs) are pervasively transcribed and critical regulators of the epigenome[1, 2]. These long, polyadenylated RNAs do not code for proteins, but function directly as RNAs, recruiting chromatin modifiers to mediate transcriptional changes in processes ranging from X-inactivation (XIST) to imprint...

journal_title：RNA biology

authors： Hung T,Chang HY

更新日期：2010-09-01 00:00:00

abstract：:In an earlier report two P-transposon insertion alleles of the noncoding hsromega gene, hsromega(05241) and P292 were shown to enhance neurodegeneration caused by expression of ataxin-1 protein with expanded poly-Q in a Drosophila model. In present study, we examined the possible relation between hsromega gene express...

journal_title：RNA biology

authors： Sengupta S,Lakhotia SC

更新日期：2006-01-01 00:00:00

abstract：:Viroids are the smallest autonomous infectious nucleic acids known today. They are non-coding, unencapsidated, circular RNAs with sizes ranging from 250 to 400 nucleotides and infect certain plants. These RNAs are transcribed by rolling-circle mechanisms in the plant host's nuclei (Pospiviroidae) or chloroplasts (Avsu...

journal_title：RNA biology

authors： Hammann C,Steger G

更新日期：2012-06-01 00:00:00

abstract：:MicroRNAs are small RNAs that post-transcriptionally regulate eukaryotic gene expression. In addition to their involvement in a wide range of physiological and pathological processes, including viral infections, microRNAs are increasingly implicated in the eukaryotic response to bacterial pathogens. Recent studies hav...

journal_title：RNA biology

authors： Eulalio A,Schulte L,Vogel J

更新日期：2012-06-01 00:00:00

abstract：:We previously showed that miR-122 was frequently downregulated in hepatocellular carcinoma (HCC) and C/EBPα transactivated miR-122 expression. In this study, we found that Sp1 bound to the miR-122 promoter at two different sites. Interestingly, either inhibition or overexpression of Sp1 could decrease the miR-122 prom...

journal_title：RNA biology

authors： Zeng C,Sang Y,Wang FY,Zhuang SM

更新日期：2020-02-01 00:00:00

abstract：:In mammals, the genic disequilibrium between males (XY) and females (XX) is resolved through the inactivation of one of the X-chromosomes in females. X-chromosome inactivation (XCI) takes place in all mammalian species, but has mainly been studied in the mouse model where it was shown to be controlled by the interplay...

journal_title：RNA biology

authors： Vallot C,Rougeulle C

更新日期：2013-08-01 00:00:00