Abstract:
:Significant structural and mechanistic similarities between the spliceosomal snRNAs and catalytically critical domains of self-splicing group II introns have led to the hypothesis that the spliceosomes and group II introns may be evolutionarily related. We have previously shown that in vitro-transcribed, protein-free U6 and U2 snRNAs can catalyze a two-step splicing reaction in trans on two short RNA oligonucleotides that is identical to the splicing reactions performed by many self-splicing group II introns. Here we show that the same two snRNAs can perform splicing in cis by removal of an intervening sequence from a model substrate. These results prove that the protein-free snRNAs are competent to perform splicing on pre-mRNAs and further strengthen the possibility of an evolutionary relationship to group II introns.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Jaladat Y,Zhang B,Mohammadi A,Valadkhan Sdoi
10.4161/rna.8.3.15386subject
Has Abstractpub_date
2011-05-01 00:00:00pages
372-7issue
3eissn
1547-6286issn
1555-8584pii
15386journal_volume
8pub_type
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