Abstract:
:Two different isolates of the myxomycete Didymium iridis harbour homing endonuclease genes that are expressed from group I introns inserted into identical sites within the small subunit ribosomal DNA. The homing endonuclease proteins are related in sequence, and their gene structures share similar features such as the presence of small spliceosomal introns and functional polyadenylation sites. However, they are transcribed from opposite strands of the ribosomal DNA and presumable by different RNA polymerases. We have previously described the in vivo expression of the I-DirI homing endonuclease from within the ribosomal RNA precursor. In this paper, we demonstrate the in vivo expression of the I-DirII homing endonuclease from the opposite strand of the Didymium rRNA gene. A comparison of the expression strategies of the two genes demonstrates the feasibility of antisense expression and provides insight into nucleolar gene expression.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Johansen SD,Vader A,Sjøttem E,Nielsen Hdoi
10.4161/rna.3.4.3958subject
Has Abstractpub_date
2006-10-01 00:00:00pages
157-62issue
4eissn
1547-6286issn
1555-8584pii
3958journal_volume
3pub_type
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