Abstract:
:MicroRNAs are essential for spermatogenesis. However, the stage-specific requirements for particular miRNAs in the male mammalian germ line remain largely uncharacterized. The miR-34 family is, to date, the only miRNA proven to be necessary for the production of sperm in mammals, though its germline roles are poorly understood. Here, we generate and analyze paired small RNA and mRNA profiles across different stages of germline development in male mice, focusing on time points shortly before and during meiotic prophase I. We show that in addition to miR-34, miR-29 also mediates widespread repression of mRNA targets during meiotic prophase I in the male mouse germline. Furthermore, we demonstrate that predicted miR-29 target mRNAs in meiotic cells are largely distinct from those of miR-34, indicating that miR-29 performs a regulatory function independent of miR-34. Prior to this work, no germline role has been attributed to miR-29. To begin to understand roles for miR-29 in the germ line, we identify targets of miR-29 undergoing post transcriptional downregulation during meiotic prophase I, which likely correspond to the direct targets of miR-29. Interestingly, candidate direct targets of miR-29 are enriched in transcripts encoding extracellular matrix components. Our results implicate the miR-29 family as an important regulatory factor during male meiosis.
journal_name
RNA Bioljournal_title
RNA biologyauthors
Hilz S,Fogarty EA,Modzelewski AJ,Cohen PE,Grimson Adoi
10.1080/15476286.2016.1270002subject
Has Abstractpub_date
2017-02-01 00:00:00pages
219-235issue
2eissn
1547-6286issn
1555-8584journal_volume
14pub_type
杂志文章相关文献
RNA Biology文献大全abstract::Non-coding RNA (ncRNA) molecules have fundamental roles in cells and many are also stable in body fluids as extracellular RNAs. In this study, we used RNA sequencing (RNA-seq) to investigate the profile of small non-coding RNA (sncRNA) in human serum. We analyzed 10 billion Illumina reads from 477 serum samples, inclu...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2017.1403003
更新日期:2018-02-01 00:00:00
abstract::RNA interference (RNAi) is a powerful tool for gene functional analysis of plant-parasitic nematodes (PPNs). RNAi involving soaking in a dsRNA solution and in planta methods is commonly applied in the study of gene function in PPNs. However, certain problems restrict the application of these methods. Therefore, more c...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1852779
更新日期:2020-12-14 00:00:00
abstract::Trans-activating CRISPR (tracr) RNA is a distinct RNA species that interacts with the CRISPR (cr) RNA to form the dual guide (g) RNA in type II and subtype V-B CRISPR-Cas systems. The tracrRNA-crRNA interaction is essential for pre-crRNA processing as well as target recognition and cleavage. The tracrRNA consists of a...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2018.1493331
更新日期:2019-04-01 00:00:00
abstract::Interactions between glioma cells and their local environment are critical determinants of brain tumor growth, infiltration and neovascularisation. Communication with host cells and stroma via microvesicles represents one pathway by which tumors can modify their surroundings to achieve a tumor-permissive environment. ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.25281
更新日期:2013-08-01 00:00:00
abstract::The non-coding roX1 and roX2 RNAs are components of the MSL dosage compensation complex in Drosophila. We found that multiple species of roX2 RNA are produced by alternative splicing, with one major and at least 20 different minor forms associated with MSL proteins. The alternative forms are generated by variable usag...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.2.4.2473
更新日期:2005-10-01 00:00:00
abstract::The acquisition of antibiotic resistance by human pathogens poses a significant threat to public health. The mechanisms that control the proliferation and expression of antibiotic resistance genes are not yet completely understood. The aminoglycosides are a historically important class of antibiotics that were introdu...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.25757
更新日期:2013-08-01 00:00:00
abstract::The innate immune system is the first line of defense against microbial pathogens, but tight regulation of gene expression is necessary to prevent the detrimental effects of unrestrained activation. Although the functions of most long noncoding RNAs (lncRNAs; >200 nucleotides) are unknown, many have been shown to regu...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.29937
更新日期:2014-01-01 00:00:00
abstract::In metazoan organisms, alternative splicing is a central mechanism for the regulation of gene expression. However, many questions remain about the underlying molecular mechanisms. Our recent work suggests that U1 snRNP-independent premRNA splicing occurs in humans, which contributes to the regulation of alternative sp...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.6.4.9318
更新日期:2009-09-01 00:00:00
abstract::Differential mRNA stability is an important mechanism for regulation of virulence factors in Streptococcus pyogenes (group A streptococcus, GAS), a serious and prevalent human pathogen. We have described 2 Classes of mRNA in GAS that are distinguishable by 1) stability in the stationary phase of growth, 2) kinetics of...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.7.5.13097
更新日期:2010-09-01 00:00:00
abstract::Proper base-pairing of a miRNA with its target mRNA is a key step in miRNA-mediated mRNA repression. RNA remodelling by RNA-binding proteins (RBPs) can improve access of miRNAs to their target mRNAs. The largest isoform p45 of the RBP AUF1 has previously been shown to remodel viral or AU-rich RNA elements. Here, we sh...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1822637
更新日期:2020-09-29 00:00:00
abstract::In a series of conceptual articles published around the millennium, Carl Woese emphasized that evolution of cells is the central problem of evolutionary biology, that the three-domain ribosomal tree of life is an essential framework for reconstructing cellular evolution, and that the evolutionary dynamics of functiona...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.27673
更新日期:2014-01-01 00:00:00
abstract::RNase J is a prokaryotic 5'-3' exo/endoribonuclease that functions in mRNA decay and rRNA maturation. Here, we report a novel duplex unwinding activity of mpy-RNase J, an archaeal RNase J from Methanolobus psychrophilus, which enables it to degrade duplex RNAs with hairpins up to 40 bp when linking a 5' single-strande...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2020.1777379
更新日期:2020-10-01 00:00:00
abstract::The two major steps of gene expression are transcription and translation. While hundreds of studies regarding the effect of sequence features on the translation elongation process have been published, very few connect sequence features to the transcription elongation rate. We suggest, for the first time, that short tr...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2017.1384118
更新日期:2018-01-02 00:00:00
abstract::Viroids are the smallest autonomous infectious nucleic acids known today. They are non-coding, unencapsidated, circular RNAs with sizes ranging from 250 to 400 nucleotides and infect certain plants. These RNAs are transcribed by rolling-circle mechanisms in the plant host's nuclei (Pospiviroidae) or chloroplasts (Avsu...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.19810
更新日期:2012-06-01 00:00:00
abstract::The RNA component of the RNase P complex is found throughout most branches of the tree of life and is principally responsible for removing the 5' leader sequence from pre-tRNA transcripts during tRNA maturation. RNase P RNA has a number of universal core features, however variations in sequence and structure found in ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.21502
更新日期:2012-09-01 00:00:00
abstract::tRNA molecules undergo extensive post-transcriptional processing to generate the mature functional tRNA species that are essential for translation in all organisms. These processing steps include the introduction of numerous specific chemical modifications to nucleotide bases and sugars; among these modifications, met...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.1080/15476286.2015.1008358
更新日期:2015-01-01 00:00:00
abstract::Processing bodies (P-bodies) are cytoplasmatic mRNP granules containing non-translating mRNAs and proteins from the mRNA decay and silencing machineries. The mechanism of P-body assembly has been typically addressed by depleting P-body components. Here we apply a complementary approach and establish an automated cell-...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.26851
更新日期:2013-11-01 00:00:00
abstract::RNA-binding proteins (RBPs) are key regulators of posttranscriptional processes such as RNA maturation, localization, turnover and translation. Despite their dysregulation in various diseases including cancer, the landscape of RBP expression in human cancer has not been well elucidated. Here, we built a comprehensive ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1673657
更新日期:2020-02-01 00:00:00
abstract::Genuine La and La-related proteins group 7 (LARP7) bind to the non-coding RNAs transcribed by RNA polymerase III (RNAPIII), which end in UUU-3'OH. The La motif and RRM1 of these proteins (the La module) cooperate to bind the UUU-3'OH, protecting the RNA from degradation, while other domains may be important for RNA fo...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.23608
更新日期:2013-03-01 00:00:00
abstract::One of the many important consequences that temperature down-shift has on cells is stabilization of secondary structures of RNAs. This stabilization has wide-spread effects, such as inhibition of expression of several genes due to termination of their transcription and inefficient RNA degradation that adversely affect...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.7.6.13482
更新日期:2010-11-01 00:00:00
abstract::Editing by ADAR enzymes is essential for mammalian life. Still, knowledge of the spatio-temporal editing patterns in mammals is limited. By use of 454 amplicon sequencing we examined the editing status of 12 regionally extracted mRNAs from porcine developing brain encompassing a total of 64 putative ADAR editing sites...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.21082
更新日期:2012-08-01 00:00:00
abstract::The synthesis of RNA is highly regulated at all stages by transcription factors. As an essential transcription elongation factor, NusA has been studied biochemically for more than 30 years. However, until now no structural information has been available on NusA-RNAP complexes and the NusA interaction site on RNAP was ...
journal_title:RNA biology
pub_type: 杂志文章,评审
doi:10.4161/rna.7.3.12021
更新日期:2010-05-01 00:00:00
abstract::Single-stranded oligonucleotides (ssOligos) are efficiently taken up by living cells without the use of transfection reagents. This phenomenon called 'gymnosis' enables the sequence-specific silencing of target genes in various types of cells. Several antisense ssOligos are used for the treatment of human diseases. Ho...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2017.1302641
更新日期:2017-11-02 00:00:00
abstract::Selenoproteins are a unique class of proteins that contain the 21st amino acid, selenocysteine (Sec). Addition of Sec into a protein is achieved by recoding of the UGA stop codon. All 25 mammalian selenoprotein mRNAs possess a 3' UTR stem-loop structure, the Selenocysteine Insertion Sequence (SECIS), which is required...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1653681
更新日期:2019-12-01 00:00:00
abstract::Ribosome biogenesis is an essential process initiated in the nucleolus. In eukaryotes, multiple ribosome biogenesis factors (RBFs) can be found in the nucleolus, the nucleus and in the cytoplasm. They act in processing, folding and modification of the pre-ribosomal (r)RNAs, incorporation of ribosomal proteins (RPs), e...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2016.1154252
更新日期:2016-01-01 00:00:00
abstract::Heterogeneous nuclear ribonucleoprotein L (hnRNP L) is a multifunctional RNA-binding protein that is involved in many different processes, such as regulation of transcription, translation, and RNA stability. We have previously characterized hnRNP L as a global regulator of alternative splicing, binding to CA-repeat, a...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.4161/rna.27991
更新日期:2014-01-01 00:00:00
abstract::RNA methylation, catalysed by a set of RNA methyltransferases (RNMTs), modulates RNA structures, properties, and biological functions. RNMTs are increasingly documented to be dysregulated in various human diseases, particularly developmental disorders and cancer. However, the genomic and transcriptomic alterations of ...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1708549
更新日期:2020-04-01 00:00:00
abstract::Oncogenic viruses are associated with approximately 15% of human cancers. In viral infections, microRNAs play an important role in host-pathogen interactions. miR-21 is a highly conserved non-coding RNA that not only regulates the development of oncogenic viral diseases, but also responds to the regulation of intracel...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2021.1880756
更新日期:2021-01-27 00:00:00
abstract::Deinococcus radiodurans is a highly stress resistant bacterium that encodes universal as well as lineage-specific factors involved in DNA transcription and repair. However, the effects of DNA lesions on RNA synthesis by D. radiodurans RNA polymerase (RNAP) have never been studied. We investigated the ability of this R...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2019.1656027
更新日期:2019-12-01 00:00:00
abstract::Altered expression levels of protein-coding genes and microRNAs have been implicated in the pathogenesis of Huntington's disease (HD). The involvement of other ncRNAs, especially long ncRNAs (lncRNA), is being realized recently and the related knowledge is still rudimentary. Using small RNA sequencing and PCR arrays w...
journal_title:RNA biology
pub_type: 杂志文章
doi:10.1080/15476286.2018.1534524
更新日期:2018-01-01 00:00:00