A bioassay for parathyroid hormone and other substances affecting bone metabolism.

Abstract:

:A bioassay method for measuring parathyroid hormone (PTH) and other substances that influence bone metabolism is described. Monolayers of bone cells are culturedin vitro, then treated with the test material. Biological activity is determined by measuring the resulting rise in bone-cell adenosine 3',5'-monophosphate (cAMP).Treatment with PTH (1-500 ng/ml) produced a dose-dependent increase in bone-cell cyclic AMP levels. Treatment with prostaglandin E2 also caused an elevation in bone-cell cyclic AMP level, whereas oestradiol inhibited the effect of PTH. High calcium concentration inhibited the stimulatory effect of PTH on bone-cell cyclic AMP; variations in phosphate and magnesium concentrations were without effect. Biological activity was demonstrated in various unextracted normal and hypercalcaemic sera.

journal_name

Ir J Med Sci

authors

McPartlin J,Powell D

doi

10.1007/BF02938966

subject

Has Abstract

pub_date

1976-12-01 00:00:00

pages

319

issue

1

eissn

0021-1265

issn

1863-4362

pii

10.1007/BF02938966

journal_volume

145

pub_type

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