Abstract：
:This study investigated the expression of the neonatal Fc receptor (FcRn) in maternal blood, cord blood and placental cells and determined IgG levels in maternal blood and cord blood from diabetic mothers. Peripheral blood, cord blood and placenta samples were collected from 26 mothers with normoglycaemia (non-diabetic, ND group) and 52 with hyperglycaemia (26 with mild gestational hyperglycaemia, MGH group, and 26 with type 2 diabetes mellitus, DM-2 group). Cells expressing CD19(+) and FcRn were identified by flow cytometry. Total IgG and its subclasses were quantified by ELISA. Maternal blood from DM-2 and cord blood from MGH exhibited a higher proportion of CD19(+) expression by B cells. DM-2 showed a lower proportion of CD19(+) cells in placenta. FcRn expression increased in cells from cord blood and placenta from MGH. Maternal blood, cord blood and placenta cells from DM-2 showed lower FcRn expression. Blood IgG levels were lower in DM-2, and cord blood IgG levels were higher in MGH. The highest levels of IgG4 were detected in the blood of hyperglycaemic mothers. The highest IgG3 and IgG4 levels in cord blood were detected in MGH, and the lowest IgG2 and IgG3 levels in DM-2. Maternal hyperglycaemia compromised placental transfer of IgG1, IgG3 and IgG4. The results suggest that regardless of hyperglycaemia degree, it decreases FcRn expression in placenta and blood cells and compromises the production and transfer of antibodies from maternal blood to newborns.
journal_name
Scand J Immunoljournal_title
Scandinavian journal of immunologyauthors
de Souza EG,Hara CC,Fagundes DL,de Queiroz AA,Morceli G,Calderon IM,França EL,Honorio-França ACdoi
10.1111/sji.12466subject
Has Abstractpub_date
2016-10-01 00:00:00pages
237-44issue
4eissn
0300-9475issn
1365-3083journal_volume
84pub_type
杂志文章abstract：:Assessment of cytokine expression has become crucial to understand host responses to infections as well as autoimmunity. Several approaches including Northern blot, RNase protection assay and enzyme-linked immunosorbent assay have been used for this purpose, but they are time consuming, labour intense, and relatively ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2003.01250.x
更新日期：2003-05-01 00:00:00
abstract：:After 3-5 days of in vitro culture, peritoneal cells from untreated C3H mice produce autoantibodies specific for autoantigens in the membranes of mouse erythrocytes. The autoantigens are exposed in vitro by treating mouse erythrocytes with the proteolytic enzyme bromelain. Limiting-dilution cell culture techniques wer...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1984.tb01035.x
更新日期：1984-12-01 00:00:00
abstract：:Sixteen plasma proteins were studied in the supernatants from 20 successive washings of human leucocytes by a new sensitive immunoelectrophoretic method. In the first few supernatants a fast and parallel decrease in concentration of all proteins took place, but for 11 proteins this was followed by a phase of slow decr...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1982.tb00664.x
更新日期：1982-04-01 00:00:00
abstract：:The autologous mixed lymphocyte reaction (AMLR) and T-cell subsets defined with monoclonal antibodies were examined in the peripheral blood of six patients with chronic mucocutaneous candidiasis (CMC). The AMLR was deficient in four of six patients when compared with simultaneously studied healthy controls. These pati...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：
更新日期：1985-06-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1990.tb03216.x
更新日期：1990-12-01 00:00:00
abstract：:B-Lymphocyte-activating factor (BAFF/BLyS) is a survival factor for B cells, belonging to the tumor necrosis ligand super family. Serum BAFF levels have been found to be elevated in patients with systemic lupus erythematosus (SLE). Neutralization of BAFF activity was suggested as an additional therapeutic approach in ...
journal_title：Scandinavian journal of immunology
pub_type： 临床试验,杂志文章
doi：10.1111/j.1365-3083.2006.01737.x
更新日期：2006-04-01 00:00:00
abstract：:The advent of advanced molecular biological techniques in the last two decades has allowed the study of genetic factors in inflammatory bowel disease (IBD). A variety of techniques have been employed to elucidate the effects of genes, starting with the clinical observations that IBD is more common in the relatives of ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1046/j.1365-3083.2000.00656.x
更新日期：2000-01-01 00:00:00
abstract：:We evaluated the B-cell response in cerebrospinal fluid (CSF) and blood by enumerating cells secreting antibodies to myelin-associated glycoprotein (MAG) and, for reference, to myelin basic protein (MBP), two myelin components which may constitute targets for autoimmune attack in multiple sclerosis (MS). Among 25 untr...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1991.tb02493.x
更新日期：1991-01-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2011.02535.x
更新日期：2011-07-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb02677.x
更新日期：1975-01-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1977.tb02141.x
更新日期：1977-01-01 00:00:00
abstract：:Antigen-binding peripheral blood leukocytes (PBL) from normal and bursectomized agammaglobulinemic chickens were labeled by incubation in vitro with radioiodinated antigen at 4 degrees C in the presence of sodium azide. [125I]TGAL-binding cells could be detected by autoradiography of PBL from normal, unimmunized chick...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1975.tb02612.x
更新日期：1975-01-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2001.00961.x
更新日期：2001-07-01 00:00:00
abstract：:Patients with juvenile idiopathic arthritis (JIA) have been shown to have elevated levels of circulating immune complexes (CICs) which correlated with disease activity. Our aim was to assess B cell activity by measuring the amount of and the kappa:lambda chain immunoglobulin light (L) chain ratio in CICs from JIA pati...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2006.01859.x
更新日期：2007-01-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2010.02378.x
更新日期：2010-04-01 00:00:00
abstract：:We have recently established chronic relapsing experimental autoimmune encephalomyelitis (CR-EAE) in SJL mice with a modified protocol. In this model, splenectomy aborts the relapsing-remitting course of the disease, and adoptive transfer of lymphocytes of the local draining lymph nodes (LNs) to naive recipients exace...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1046/j.1365-3083.2002.01132.x
更新日期：2002-09-01 00:00:00
abstract：:Spleens from newborn mice less than 6-7 days of age contain 'naturally occurring' suppressor cell populations that are able to inhibit nonspecifically immune responses of third-party adult spleen cells and alloreactivity in the newborn spleen per se. Isolation of the effectors of this suppressor activity reveals that ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1983.tb00811.x
更新日期：1983-05-01 00:00:00
abstract：:The relationship of velocity of blood flow to density and microanatomical distribution of inflammatory cells in the dermis was studied in 20 human tuberculin tests. Most positive reactions showed maximal blood flow velocities (measured as red blood cell (RBC) flux) at the centre of the reaction, but the two most inten...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb01096.x
更新日期：1989-01-01 00:00:00
abstract：:Lymphatic filariasis, a mosquito-borne parasitic disease, affects more than 120 million people worldwide. Vaccination for filariasis by targeting different stages of the parasite will be a boon to the existing MDA efforts of WHO which required repeated administration of the drug to reduce the infection level and susta...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/sji.12340
更新日期：2015-10-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1995.tb03630.x
更新日期：1995-07-01 00:00:00
abstract：:The purpose of this study was to investigate interleukin 2 (IL-2) and gamma interferon (IFN-gamma) production by purified CD4+ and CD8+ cells isolated from peripheral blood (PB) and synovial fluid (SF) of patients with rheumatoid arthritis (RA) and other inflammatory arthritides (non-RA). CD4+ and CD8+ cells were sele...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb02467.x
更新日期：1989-11-01 00:00:00
abstract：:Natural killer (NK) cells form part of the vertebrate defence against viruses and tumours, but show only limited specificity. The molecule(s) recognized by NK cells on target cells are at present unknown. Major histocompatibility complex (MHC) class I antigen concentration on target cells is inversely correlated with ...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1989.tb01184.x
更新日期：1989-07-01 00:00:00
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journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1981.tb00121.x
更新日期：1981-01-01 00:00:00
abstract：:Mouse macrophages were cultured on chemically modified plastic dishes. On dishes covered with immobilized glycans, the macrophages were stimulated as judged by increased 14C-glucosamine incorporation, increased cytostatic and cytolytic capacities and by morphology as seen by scanning electron microscopy. The correspon...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1984.tb01013.x
更新日期：1984-10-01 00:00:00
abstract：:The occurrence of D-D recombination during the embryonic differentiation of chicken B cells was studied. Ig heavy (H) chains were amplified by polymerase chain reaction from day-12 bursal cDNA, and 30 random V-D-J regions were analysed by DNA sequencing. No gene conversion events were observed in any of the V regions,...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1991.tb02524.x
更新日期：1991-05-01 00:00:00
abstract：:L-selectin, a cell surface glycoprotein expressed on lymphocytes, granulocytes, and monocytes, has been implicated in lymphocyte homing and extravasation of phagocytic leukocytes into areas of inflammation. Considerable differences of L-selectin expression among various individuals has been reported, with clinical cor...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：
更新日期：1994-01-01 00:00:00
abstract：:Human monocytes activated with lymphokines after 3-7 days of in vitro maturation released stable cytostatic factor(s) (CF), as determined by reduction of [methyl-3H]thymidine incorporation and cell counts in target cell cultures. Lipopolysaccharide from Escherichia coli slightly enhanced the release of CF. The CF rele...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1982.tb00654.x
更新日期：1982-03-01 00:00:00
abstract：:Medial histocompatibility (H) antigens are weak H antigens, recognized by unrestricted T cells; they differ thus from both major and minor H antigens. An example, Qed-1, is described in detail, and other known medial H antigens of the mouse are reviewed. The structural and genetic relationships of major and medial H a...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章,评审
doi：10.1111/j.1365-3083.1981.tb00607.x
更新日期：1981-12-01 00:00:00
abstract：:TIM-3 is a member of the TIM (T-cell immunoglobulin domain and mucin domain) family, which plays an important role in TH1 responses and autoimmune diseases. In this study, we cloned and characterized the porcine TIM-3 gene. Real-time PCR showed little expression of porcine TIM-3 in muscle and stomach, low expression i...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.2010.02476.x
更新日期：2011-01-01 00:00:00
abstract：:Administration of cyclophosphamide (Cy: 150 mg/kg i.p.) to rats 48 h before immunization with a T-dependent antigen (ovalbumin) resulted in a striking absolute eosinophilia in blood, bone marrow, and secondary lymphoid organs after 10 to 14 days. This eosinophilia was preceded by a significant increase in the W3/25+/O...
journal_title：Scandinavian journal of immunology
pub_type： 杂志文章
doi：10.1111/j.1365-3083.1987.tb02274.x
更新日期：1987-10-01 00:00:00
