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Stimulatory effect of immobilized glycans on macrophages in vitro.

Abstract：

:Mouse macrophages were cultured on chemically modified plastic dishes. On dishes covered with immobilized glycans, the macrophages were stimulated as judged by increased 14C-glucosamine incorporation, increased cytostatic and cytolytic capacities and by morphology as seen by scanning electron microscopy. The corresponding soluble glycans did not have the capacity to stimulate macrophages as measured by these criteria. Plastic surfaces covered with polyethylenimine showed stimulation of the macrophages with regard to some of the parameters measured. These results may indicate that the stimulation is a multistep process and that, contrary to earlier findings, it is not a prerequisite for stimulation that the glycan be intracellular. The results support the idea that a fixed steric arrangement of glycans is necessary for the stimulation of macrophages in vitro.

journal_name

Scand J Immunol

journal_title

Scandinavian journal of immunology

authors

Bøgwald J,Gouda I,Hoffman J,Larm O,Larsson R,Seljelid R

doi

10.1111/j.1365-3083.1984.tb01013.x

subject

Has Abstract

pub_date

1984-10-01 00:00:00

pages

355-60

issue

4

eissn

0300-9475

issn

1365-3083

journal_volume

20

pub_type

杂志文章

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